Fiber based enzyme-linked immunosorbent assay for C-reactive protein

Sadir, Sahba; Prabhakaran, Molamma P.; Wicaksono, Dedy H. B.; Ramakrishna, Seeram

doi:10.1016/j.snb.2014.08.051

Cited by 31 publications

(19 citation statements)

References 41 publications

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“…The three‐dimensional structure of the electrospun e.NFMs provides promising surface area for the immobilization of higher amounts of protein with the ability to enhance the signal intensity of designed immunoassay. Such an excellent feature stems from the high surface to volume ratio of the e.NFM, which provide shorter free path for protein molecules to bind with the fiber surfaces …”

Section: Resultsmentioning

confidence: 99%

“…Therefore, antigen molecules were able to bind to EDC/NHS modified e.NFM surface mainly via two different mechanisms: first, through condensation reaction between the amino groups of the antigen and COOH groups of e.NFMs and the other through physical adsorption. PLGA and PLLA membranes allow the detection of SEB antigen at higher dilutions compared with other membranes.…”

Section: Resultsmentioning

confidence: 99%

“…Such an excellent feature stems from the high surface to volume ratio of the e.NFM, which provide shorter free path for protein molecules to bind with the fiber surfaces. 38 The contact angle degree of PLLA, PLGA, PCL, and PES e.NFMs is shown in Figure 3. All contact angles measured on the polymeric nanofiber membranes are over 100°, which means that all surfaces are hydrophobic.…”

Section: Immunoassay Running Through Microfluidic Channelmentioning

confidence: 99%

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Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

Mahmoudifard

Vossoughi

Soleimani

2019

Polymers for Advanced Techs

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Protein capturing on polymeric substrate of microfluidic devices is a key factor for the fabrication of immunoassay with high sensitivity. In this work, simple and versatile technique of electrospinning was used to produce electrospun nanofibrous membranes (e.NFMs) with high surface area as a substrate for microfluidic‐based immunoassay to increase sensitivity. It was found that the simultaneous use of e.NFM and 1‐Ethylethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐Hydroxysuccinimide hydroxysuccinimide as coupling agent has synergic effect on antigen immobilization onto the microchannels. It was found that the oxygen plasma technique for the creation of oxygen containing functional group like carboxyl and hydroxyl causes extreme leakage of solution through the microchannels. Thus, due to capillary effect, it is impossible to use hydrophilic substrate to modify microchannels. In order to compensate this problem, it is propose to utilize other type of polymer for the fabrication of nanofiber to answer this important question that if it is possible to enhance the sensitivity of immunoassay just by changing the polymer type? For this purpose, four different polymers, namely, polycaprolactone, poly lactic‐co‐glycolic acid, poly L‐lactic acid, and polyethersolfone were used as the based material for e.NFM fabrication. Results showed that compared with plain poly (dimethylsiloxane) surface of microchannels, poly lactic‐co‐glycolic acidand poly L‐lactic acid, which inherently contain end‐group of carboxyl in their chemical structure, can improve the protein immobilization, which leads to immunoassay signal enhancement through 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐hydroxysuccinimide coupling chemistry, significantly.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Immunoassay Running Through Microfluidic Channelmentioning

confidence: 99%

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Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

Mahmoudifard

Vossoughi

Soleimani

2019

Polymers for Advanced Techs

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“…Three-dimensional structure of the electrospun nanofibrous membrane provide promising surface properties for immobilization of higher amounts of primary antibody with ability to enhance the electron transfer due to the high surface to volume ratio of the nanofibers as it was also reported in other study. 24 As BET data revealed with reducing the diameter of nanofibrous membranes, specific surface area of membrane tend to increase which directly influence the amount of protein adsorption hence the immunoassay performance. Figure 5c depicts the effect of nanofiber diameter on immunoassay signal obtained from 5 mg/mL antibody adsorption through two different mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

et al. 2017

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In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency. Therefore, microfluidic immunoassays can be efficiently used as a point-of-care device in medical diagnosis. Surprisingly, we found the increase of specific surface areas of the microfluidic channels improve density of immobilized proteins and leads to higher signal strength. Anti-staphylococcus enterotoxin B (anti-SEB) was used as an analyte model to demonstrate the utility of our proposed platform. Fluorescent microscopy, Fourier transform infrared spectroscopic (FTIR), gas adsorption, contact angle, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Uv-Vis spectrophotometer and atomic force microscopy (AFM) techniques were used to assess the efficacy of antibody immobilization on the surface. To understand dominant mechanism of protein immobilization, zeta potential measurement was also carried out and it was found electrostatic attraction play significant role in antibody immobilization running into micro- channels containing through EDC/NHS. Moreover, incorporation of nanofibrous membrane causes significant improvement in the signal detection of microfluidic based immunoassay. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1108-1120, 2018.

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“…Recently, it has been reported that the incorporation of CNTs into nanofibrous structures is an innovative method to stabilising the CNTs on the electrode surface and it is suitable for sensing applications due to large surface area, good interconnectivity and high porosity [11,57]. Based on that, a sandwich-type nanostructured immunosensor based on carboxylated MWCNT (CMWCNT)-embedded whiskered nanofibres (WNFs) for the detection of cardiac cTnI has been developed [11].…”

Section: Cardiac Troponin T (Ctnt) and I (Ctni)mentioning

confidence: 99%

Nanomaterials‐based Electrochemical Sensing of Cardiac Biomarkers for Acute Myocardial Infarction: Recent Progress

Nsabimana

Yuan

et al. 2018

Electroanalysis

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The development of the methods for early and accurate diagnosis of acute myocardial infarction are needed to facilitate immediate treatment of patients. One of the ways to achieve that is the detection of cardiac biomarkers for myocardial infarction, such as thrombin, cardiac troponins (I and T), myoglobin, etc. Nanotechnology has played an important role in the development of sensitive and efficient electrochemical sensors for cardiac biomarkers. In this review, we discuss recent progress on nanomaterial‐based electrochemical sensing of various cardiac biomarkers for acute myocardial infarction.

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Fiber based enzyme-linked immunosorbent assay for C-reactive protein

Cited by 31 publications

References 41 publications

Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

Nanomaterials‐based Electrochemical Sensing of Cardiac Biomarkers for Acute Myocardial Infarction: Recent Progress

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