Fiber-optic probe for in vivo measurement of oxygen partial pressure

Peterson, John I.; Fitzgerald, R. V.; Buckhold, Delwin K.

doi:10.1021/ac00265a017

Cited by 303 publications

(109 citation statements)

References 18 publications

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“…Oxidases, which catalyze the oxidation of compounds using oxygen, are usually co-immobilized with oxygen-sensitive luminescent materials, such as polycyclic aromatic hydrocarbons [12][13][14], porphyrins [15], and transition metal complexes [16][17][18]. Among them, the ruthenium diimine complexes, such as Ru(phen) 3 Cl 2 , and Ru(dpp) 3 Cl 2 offer definite advantages including high photostability, long excited-state lifetimes, high quantum yields, and high quenching rate constants [19][20][21].…”

Section: Enzymesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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(ABSTRACT)Fiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures.To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity

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Section: Enzymesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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“…Furthermore optrode measurements are not disturbed by electromagnetic fields. Oxygen optrodes have been developed mainly as minisensors for use in blood gas analysis and as macrosensors for environmental monitoring and in biotechnological applications (Peterson et al 1984;Weigl et al 1994). We describe here a new fiberoptic oxygen microsensor, based on dynamic fluorescence quenching, for measuring oxygen gradients at high spatial resolution.…”

Section: Fiber-optic Oxygen Microsensors a New Tool In Aquatic Biologymentioning

confidence: 99%

Fiber‐optic oxygen microsensors, a new tool in aquatic biology

Klimant

Meyer

Kühl

1995

Limnology & Oceanography

350

249

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A new fiber‐optic oxygen microsensor (microoptrode) based on dynamic fluorescence quenching has been developed to measure oxygen gradients in marine sediments and microbial mats. The microoptrodes are fabricated by immobilizing an oxygen‐quenchable fluorophore at the tapered tip of an optical fiber. A special optoelectronic system has been designed to measure oxygen with these microoptrodes. It is based on small and cheap optical components and can easily be miniaturized for field applications. In contrast to oxygen microelectrodes, the new oxygen microoptrodes are easy to make, do not consume oxygen, and show no stirring dependence of the signal. In addition, they show excellent long‐term stability and storage stability. Hydrogen sulfide, carbon dioxide, and other relevant chemical parameters do not interfere with the measurement. Oxygen profiles in marine sediments obtained from measurements with microoptrodes show good correlation to profiles measured with oxygen microelectrodes.

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“…For example, Basu et al [21] synthesized a pyrene derivative: 1-decyl-4-(1-pyrenyl) butanoate 12 which has a high oxygen quenching sensitivity and lower diffusion coefficient in silicone polymer. Perylene dibutyrate 13 has been incorporated in an organic polymer adsorbent (amberlite) and deposited on an optical fiber for in vivo oxygen concentration measurements [183]. Among the different PAHs, 1-pyrenebutyric acid 10 possesses longer fluorescence lifetime (0.2 μs) and therefore it is suitable for optical oxygen sensing devices; it has been demonstrated that isolated rat liver cells easily take up 1-pyrenebutyric acid in concentrations up to 0.8 mM and the fluorescence probe can be used to measure intracellular oxygen concentration [108].…”

Section: Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Indicators for optical oxygen sensors

Borisov¹,

Quaranta²,

Klimant³

2012

Advances in Chemical Bioanalysis

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Continuous monitoring of oxygen concentration is of great importance in many different areas of research which range from medical applications to food packaging. In the last three decades, significant progress has been made in the field of optical sensing technology and this review will highlight the one inherent to the development of oxygen indicators. The first section outlines the bioanalytical fields in which optical oxygen sensors have been applied. The second section gives the reader a comprehensive summary of the existing oxygen indicators with a critical highlight on their photophysical and sensing properties. Altogether, this review is meant to give the potential user a guide to select the most suitable oxygen indicator for the particular application of interest.

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Fiber-optic probe for in vivo measurement of oxygen partial pressure

Cited by 303 publications

References 18 publications

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Fiber‐optic oxygen microsensors, a new tool in aquatic biology

Indicators for optical oxygen sensors

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