Fiber optic SPR sensor for human Immunoglobulin G measurement based on the MMF-NCF-MMF structure

Zheng, Yingfang; Lang, Tingting; Cao, Bobo; Jin, Jiajun; Dong, Ruiqi; Feng, Haoren

doi:10.1016/j.yofte.2018.10.015

Cited by 25 publications

(5 citation statements)

References 20 publications

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“…Huang et al also reported an H‐shaped optical fiber‐based SPR sensor for the specific detection of H‐IgG on Goat aH‐IgG with a detection limits of 3.4 μg/mL. Zheng et al demonstrated the detection of H‐IgG on Goat aH‐IgG by means of a fiber optic SPR sensor (multimode fiber–noncore fiber‐multimode fiber (MMF‐NCF‐MMF) achieving a limit of detection of 1.75 μg/mL [87–89].…”

Section: Resultsmentioning

confidence: 99%

Multiple protein‐patterned surface plasmon resonance biochip for the detection of human immunoglobulin‐G

Kashyap

Boro

Yasmin

et al. 2023

Journal of Biophotonics

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A portable surface plasmon resonance (SPR) measurement prototype integrated with a multiple protein‐patterned SPR biochip is introduced for label‐free and selective detection of human immunoglobulin‐G (H‐IgG). The polyclonal anti‐H‐IgG antibodies derived from goat, rabbit, and mouse were immobilized through polydimethylsiloxane (PDMS) microchannels to fabricate the patterned SPR biochip. The PDMS surface was functionalized using 3‐aminopropyltrimethoxysilane and bonded to carbodiimide‐activated gold substrates to construct irreversibly bonded hydrophilic microfluidic chip at room temperature. For SPR measurement, a custom‐made system is developed with a high angular scanning accuracy of 0.005° and a wide scanning range of 30°–80° that avoids the conventional requirement of expensive goniometric stages and detector arrays. The SPR biochip immobilized with 750 μg/mL goat anti‐H‐IgG demonstrated detection of H‐IgG with a detection limits of 15 μg/mL, and linear response through a wide concentration range (15–225 μg/mL) of high coefficient of determination (R2 = 0.99661). The selectivity of the sensor was investigated by exposing them to two different non‐specific targets (bovine serum albumin and polyvalent antivenom). The results indicate negligible sensor response towards nonspecific targets (0.25° for 30 μg/mL bovine serum albumin (BSA) and 0.25° for 30 μg/mL polyvalent antivenom) in comparison to H‐IgG (1.5° for 30 μg/mL).

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Section: Resultsmentioning

confidence: 99%

Multiple protein‐patterned surface plasmon resonance biochip for the detection of human immunoglobulin‐G

Kashyap

Boro

Yasmin

et al. 2023

Journal of Biophotonics

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“…The refractive index of the sensing medium was 1.343 before adding the PBS solution. In this work, we have considered that due to the addition of IgG in the PBS solution the refractive index of PBS solution changes from 1.343 to 1.373 near the GO surface (Zheng et al, 2018). We have taken five different concentrations 0, 0.025, 0.05, 0.1 and 0.25 mg/ml, respectively of IgG in PBS solution [42].…”

Section: Fig 2 Resonance Angle (Deg) Verses Reflectance For Optimizat...mentioning

confidence: 99%

Enhanced sensitivity of surface plasmon resonance biosensor for the selective detection of immunoglobin (IgG)

Kumar

Dubey²,

Kumar³

et al. 2022

Opt Quant Electron

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In this work, we propose a surface plasmon resonance (SPR) biosensor based on a hybrid structure of silicon nitride (Si3N4), molybdenum trioxide (MoO3), and graphene oxide (GO) in the Kretschmann configuration. In order to investigate the performance parameter of the SPR biosensor, we calculate, theoretically, the reflectance spectra of the proposed model and analysed it. The investigation of various performance parameters i.e., sensitivity, detection accuracy, and quality factor, of sensor configuration are theoretically done with the optimized thickness of silver (55 nm), silicon nitride (5 nm), molybdenum trioxide (10 nm), and graphene oxide (2.55 nm) respectively. The sensitivity, detection accuracy, and figure of merit for the proposed SPR structure is found at 301 deg./RIU, 4.01, and 133 RIU -1 respectively for the detection of IgG. It is clearly observed that the sensitivity of the proposed biosensor is enhanced by 43.2% with respect to other conventional SPR biosensors. The refractive index of the analyte changes from 1.33 to 1.37 due to adsorption of the analyte at the GO surface. The proposed sensor design is able to detect the small change in the refractive index of the sensing media doped with the analyte. Therefore, the proposed structure of the SPR sensor may have potential applications in the field of biomedical and chemical sensing applications.

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“…This sensor was further immobilized with specific antibodies and the resulted system could deliver the detection of human IgG from 0.5 to 40 μg/mL. A report described the use of an MMF–NCF–MMF structure for developing the sensor for human IgG 28 . The sensor was constructed with a noncore fiber (NCF) sandwiched between two multimode fibers (MMF).…”

Section: Introductionmentioning

confidence: 99%

L-cysteine/MoS2 modified robust surface plasmon resonance optical fiber sensor for sensing of Ferritin and IgG

Thawany

Khanna

Tiwari

et al. 2023

Sci Rep

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L-cysteine conjugated molybdenum disulphide (MoS2) nanosheets have been covalently attached to a gold coated surface plasmon resonance (SPR) optical fiber to prepare a robust and stable sensor. Owing to the multifunctionality of the deposited nanosheet conjugate, the antibodies are also covalently conjugated in the subsequent step to realize the design of a SPR optical fiber biosensor for the two important bioanalytes namely, Ferritin and Immunoglobin G (IgG). The different stages of the biosensor preparation have been characterized and verified with microscopic and spectroscopic techniques. A uniform and stable deposition of the L-cysteine/MoS2 nanosheets has allowed the biosensor to be reused for multiple times. Unlike the peeling-off of the MoS2 coatings from the gold layer reported previously in the case of physically adsorbed nanomaterial, the herein adopted strategy addresses this critical concern. It has also been possible to use the single SPR fiber for both Ferritin and IgG bioassay experiments by regenerating the sensor and immobilizing two different antibodies in separate steps. For ferritin, the biosensor has delivered a linear sensor response (SPR wavelength shifts) in the concentration range of 50–400 ng/mL, while IgG has been successfully sensed from 50 to 250 µg/mL. The limit of detection for Ferritin and IgG analysis have been estimated to be 12 ng/mL and 7.2 µg/mL, respectively. The biosensors have also been verified for their specificity for the targeted molecule only. A uniform and stable deposition of the nanomaterial conjugate, reproducibility, regeneration capacity, a good sensitivity, and the specificity can be highlighted as some of key features of the L-cysteine/MoS2 optical fiber biosensor. The system can be advocated as a useful biosensor setup for the sensitive biosensing of Ferritin and IgG.

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Fiber optic SPR sensor for human Immunoglobulin G measurement based on the MMF-NCF-MMF structure

Cited by 25 publications

References 20 publications

Multiple protein‐patterned surface plasmon resonance biochip for the detection of human immunoglobulin‐G

Multiple protein‐patterned surface plasmon resonance biochip for the detection of human immunoglobulin‐G

Enhanced sensitivity of surface plasmon resonance biosensor for the selective detection of immunoglobin (IgG)

L-cysteine/MoS2 modified robust surface plasmon resonance optical fiber sensor for sensing of Ferritin and IgG

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