Fiber-reinforced scaffolds in soft tissue engineering

Pei, Baoqing; Wang, Wei; Fan, Yubo; Wang, Xiumei; Watari, Fumio; Li, Xiaoming

doi:10.1093/rb/rbx021

Cited by 89 publications

(56 citation statements)

References 96 publications

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“…This study highlights the current types of scaffolds, their uses, advantages, and limitations in stem cell tissue engineering applications and in regenerative medicine. It also highlights 3 D scaffolds, which are the most suitable scaffolds for delivering cells in vivo; moreover, for developing engineered tissues, different mechanical property/ porosity relationships and emerging applications in soft tissue engineering have been proposed [58,59] Assessment of various variants of culture media enriched with osteoinductive agents is a valuable comparative analysis potentially applicable for further optimization of pre-clinical strategies based on growth factors and biomaterials in skeletal engineering. BMP2 and FGF2 significantly increase ASC cell mineralization and induce osteogenesis.…”

Section: Evaluation Of the Osteogenic Potential Of Cervical Fat From mentioning

confidence: 99%

Comparison of adipose stem cells sources from various locations of rat body for their application for seeding on polymer scaffolds

Kurzyk

Dębski

Święszkowski

et al. 2019

Journal of Biomaterials Science, Polymer Edition

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Adipose tissue yields adult adipose stem cells (ASCs) in large quantities via less-invasive methods. These cells are of interest owing to their modulating properties and paracrine activities, which can be harnessed in regenerative medicine. Many studies on the use of rat fat tissue in an autologous animal model have been conducted; however, the different locations to obtain stromal vascular fraction of rat fat depots have not been fully characterized. The purpose of the current study was to identify optimal source of ASC from various locations of rat body. Animal experiments in vitro revealed that fat depots from cervical fat are an optimal ASC source. A high ASC yield facilitates subsequent studies on autologous transplantation in rats. The secondary objective was to compare the efficiency of osteoinductive media composition and evaluate of osteogenic potential of ASCs for seeding on scaffolds for bone repair. Scaffolds were assessed in vitro, using rat adipose stem cells and threedimensional (3D) scaffolds comprising polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL þ 5%TCP). Seeded ASCs adhere to the surface and migrate to the scaffolds. Upon staining and determining alkaline phosphatase levels, PCL þ 5%TCP scaffolds performed better than PCL scaffolds. Furthermore, growth factors such as BMP2 and FGF2 significantly increased ASC mineralization and induced osteogenesis (p < 0.05). Our results may help select and develop pre-clinical animal model for confirming the use of ASC, alone or in association with appropriate biomaterials for bone repair. ARTICLE HISTORY

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Section: Evaluation Of the Osteogenic Potential Of Cervical Fat From mentioning

confidence: 99%

Comparison of adipose stem cells sources from various locations of rat body for their application for seeding on polymer scaffolds

Kurzyk

Dębski

Święszkowski

et al. 2019

Journal of Biomaterials Science, Polymer Edition

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“…Tissue replacement is commonly used in orthopedics for fractures, including femur, humerus, and bone socket fractures [1][2][3]. These scaffolds have been produced using a combination of both synthetic and natural polymers, such as polylactic acid (PLA), polylactic coglycolide (PLGA), polyvinyl alcohol (PVA), and acrylonitrile-butadiene-styrene (ABS) [4][5][6]. The combination of synthetic and natural polymers has been proven to not be fully biodegradable or biocompatible implant material in repairing bone fractures [7].…”

Section: Introductionmentioning

confidence: 99%

The Role of Two-Step Blending in the Properties of Starch/Chitin/Polylactic Acid Biodegradable Composites for Biomedical Applications

et al. 2020

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The current research trend for excellent miscibility in polymer mixing is the use of plasticizers. The use of most plasticizers usually has some negative effects on the mechanical properties of the resulting composite and can sometimes make it toxic, which makes such polymers unsuitable for biomedical applications. This research focuses on the improvement of the miscibility of polymer composites using two-step mixing with a rheomixer and a mix extruder. Polylactic acid (PLA), chitin, and starch were produced after two-step mixing, using a compression molding method with decreasing composition variation (between 8% to 2%) of chitin and increasing starch content. A dynamic mechanical analysis (DMA) was used to study the mechanical behavior of the composite at various temperatures. The tensile strength, yield, elastic modulus, impact, morphology, and compatibility properties were also studied. The DMA results showed a glass transition temperature range of 50 °C to 100 °C for all samples, with a distinct peak value for the loss modulus and factor. The single distinct peak value meant the polymer blend was compatible. The storage and loss modulus increased with an increase in blending, while the loss factor decreased, indicating excellent compatibility and miscibility of the composite components. The mechanical properties of the samples improved compared to neat PLA. Small voids and immiscibility were noticed in the scanning electron microscopy images, and this was corroborated by X-ray diffraction graphs that showed an improvement in the crystalline nature of PLA with starch. Bioabsorption and toxicity tests showed compatibility with the rat system, which is similar to the human system.

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“…Concerning regeneration medicine, CFs are mainly utilized to fabricate scaffolds for tissue repair. In this respect, CFs are utilized to fabricate scaffolds to enhance tissue regeneration in both soft [370] and hard tissues [371]. Cartilage tissues are a complex environment because, differently from other tissues, they are not self-healing and are rather difficult to regenerate [372].…”

Section: Carbon Fibersmentioning

confidence: 99%

“…Cartilage tissues are a complex environment because, differently from other tissues, they are not self-healing and are rather difficult to regenerate [372]. Nowadays, the majority of the scaffolds produced for cartilage repair fail because of insufficient mechanical strength and structural resilience [370]. In [373], additive 3D printing is utilized to sinter polyamide/CF composite in a computer designed complex structures.…”

Section: Carbon Fibersmentioning

confidence: 99%

“…CFs have shown good biocompatibility by both in vivo and in vitro experiments [397]. CF/carbon composites, scaffolds made of CF find application in regenerative medicine, drug delivery, nerve stimulation ( [367,370,397,398]; see Figure 20). In all these cases, the surface termination, which drives the interaction with the composite material and the living matter, is important.…”

Section: Functionalization Of Carbon Fibersmentioning

confidence: 99%

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Functionalization of Carbon Nanomaterials for Biomedical Applications

Liu

Speranza

2019

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Over the past decade, carbon nanostructures (CNSs) have been widely used in a variety of biomedical applications. Examples are the use of CNSs for drug and protein delivery or in tools to locally dispense nucleic acids to fight tumor affections. CNSs were successfully utilized in diagnostics and in noninvasive and highly sensitive imaging devices thanks to their optical properties in the near infrared region. However, biomedical applications require a complete biocompatibility to avoid adverse reactions of the immune system and CNSs potentials for biodegradability. Water is one of the main constituents of the living matter. Unfortunately, one of the disadvantages of CNSs is their poor solubility. Surface functionalization of CNSs is commonly utilized as an efficient solution to both tune the surface wettability of CNSs and impart biocompatible properties. Grafting functional groups onto the CNSs surface consists in bonding the desired chemical species on the carbon nanoparticles via wet or dry processes leading to the formation of a stable interaction. This latter may be of different nature as the van Der Waals, the electrostatic or the covalent, the π-π interaction, the hydrogen bond etc. depending on the process and on the functional molecule at play. Grafting is utilized for multiple purposes including bonding mimetic agents such as polyethylene glycol, drug/protein adsorption, attaching nanostructures to increase the CNSs opacity to selected wavelengths or provide magnetic properties. This makes the CNSs a very versatile tool for a broad selection of applications as medicinal biochips, new high-performance platforms for magnetic resonance (MR), photothermal therapy, molecular imaging, tissue engineering, and neuroscience. The scope of this work is to highlight up-to-date using of the functionalized carbon materials such as graphene, carbon fibers, carbon nanotubes, fullerene and nanodiamonds in biomedical applications.

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Fiber-reinforced scaffolds in soft tissue engineering

Cited by 89 publications

References 96 publications

Comparison of adipose stem cells sources from various locations of rat body for their application for seeding on polymer scaffolds

Comparison of adipose stem cells sources from various locations of rat body for their application for seeding on polymer scaffolds

The Role of Two-Step Blending in the Properties of Starch/Chitin/Polylactic Acid Biodegradable Composites for Biomedical Applications

Functionalization of Carbon Nanomaterials for Biomedical Applications

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