Fiber scaffolds of polysialic acid via electrospinning for peripheral nerve regeneration

Assmann, Ulrike; Szentivanyi, Andreas; Stark, Yvonne; Scheper, Thomas; Berski, Silke; Dräger, Gerald; Schuster, R. H.

doi:10.1007/s10856-010-4072-y

Cited by 16 publications

(6 citation statements)

References 22 publications

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“…[1][2][3][4][5][6] In cases of severe injuries causing complete nerve transection, surgical treatment is required. End-to-end suturing usually can solve the problem with small gaps.…”

Section: Introductionmentioning

confidence: 99%

Poly(l-lysine) modified zein nanofibrous membranes as efficient scaffold for adhesion, proliferation, and differentiation of neural stem cells

et al. 2017

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Excellent biocompatibility and bioactivity are necessary requirements for a scaffold for nerve repair and regeneration. Natural plant protein zein was chosen as the primary material and poly(L-lysine), which is composed of common amino acids in the human body, was used to modify it. Poly(L-lysine) modified zein (ZPLL) with different PLL contents of 1.46%, 3.57%, and 6.18% was synthesized and nanofibrous membranes were prepared by electrospinning. The hydrophilicity of the membranes improved with an increase of PLL content. The biodegradability of the membranes was proved by in vitro experiments. Compared with pure zein membranes, ZPLL membranes can efficiently improve cell viability, adhesion, proliferation, and differentiation of neural stem cells (NSCs) and the effect of PLL content was further investigated. The results show that when the PLL content was 3.57%, cell adhesion and proliferation proved to be the best and most differentiated toward mature neurons with extensive neurite formation and astrocytes rather than oligodendrocytes. The ZPLL was made into nerve conduits for future study and they may be a promising biomaterial for nerve repair and regeneration.

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“…[1][2][3][4][5][6] In cases of severe injuries causing complete nerve transection, surgical treatment is required. End-to-end suturing usually can solve the problem with small gaps.…”

Section: Introductionmentioning

confidence: 99%

Poly(l-lysine) modified zein nanofibrous membranes as efficient scaffold for adhesion, proliferation, and differentiation of neural stem cells

et al. 2017

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“…For example, colominic acid – a carbohydrate derivative of PSA – has been used to functionalize electrospun scaffolds and silanized glass for studies of peripheral nerve regeneration, but the results have been mostly disappointing [9, 10]. Additionally, the heterogeneity of length, high metabolic clearance, and potential for immunogenicity of colominic acid remain to be resolved [11].…”

Section: Introductionmentioning

confidence: 99%

Neural cell type-specific responses to glycomimetic functionalized collagen

et al. 2012

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Despite their noted functional role, glycans have had limited therapeutic use due to difficulties in synthesis and quick degradation in vivo. The recent discovery of glycomimetics has provided new opportunities for their application. In this study, we have functionalized type I collagen with peptide mimics of two glycans: (1) polysialic acid (PSA) and (2) an epitope first discovered on human natural killer cells (HNK-1). These glycans and their glycomimetic counterparts have been shown to be important regulators of repair following injury through their unique and phenotypically specific effects on neural behavior. We show that these molecules retain their bioactivity following functionalization to the collagen backbone. Grafted HNK-1 encouraged motor neuron outgrowth, while grafted PSA encouraged sensory and motor neuron outgrowth and enhanced Schwann cell proliferation and process extension. These data support the potential of glycomimetic-functionalized collagen as a biomaterial strategy to increase the efficiency of synaptic reconnection following nervous system injury.

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“…First attempts to construct PSA containing biomaterials for regeneration after nervous system injury made use of PSA or PSA mimetic coupled collagen fibers electrospun in fiber scaffolds which promoted proliferation of immortalized Schwann cells (Assmann et al . ). Grafted PSA mimetic collagen encouraged sensory and motor neuron outgrowth, enhanced Schwann cell proliferation and process extension and stimulated peripheral nerve repair (Masand et al .…”

mentioning

confidence: 97%

“…Peptide mimics of PSA developed by phage display screening of large libraries with PSA monoclonal antibodies (Torregrossa et al 2004;Mehanna et al 2009) promoted functional recovery and plasticity after injury of the murine peripheral and central nervous systems (Marino et al 2009;Mehanna et al 2009Mehanna et al , 2010. First attempts to construct PSA containing biomaterials for regeneration after nervous system injury made use of PSA or PSA mimetic coupled collagen fibers electrospun in fiber scaffolds which promoted proliferation of immortalized Schwann cells (Assmann et al 2010). Grafted PSA mimetic collagen encouraged sensory and motor neuron outgrowth, enhanced Schwann cell proliferation and process extension and stimulated peripheral nerve repair (Masand et al 2012a, b), indicating the potential of mimetics to replace native PSA.…”

mentioning

confidence: 99%

Nonyloxytryptamine mimics polysialic acid and modulates neuronal and glial functions in cell culture

Loers

Saini

Mishra

et al. 2013

Journal of Neurochemistry

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Polysialic acid (PSA) is a major regulator of cell-cell interactions in the developing nervous system and in neural plasticity in the adult. As a polyanionic molecule with high water-binding capacity, PSA increases the intercellular space generating permissive conditions for cell motility. PSA enhances stem cell migration and axon path finding and promotes repair in the lesioned peripheral and central nervous systems, thus contributing to regeneration. As a next step in developing an improved PSA-based approach to treat nervous system injuries, we searched for small organic compounds that mimic PSA and identified as a PSA mimetic 5-nonyloxytryptamine oxalate, described as a selective 5-hydroxytryptamine receptor 1B (5-HT 1B ) agonist. Similar to PSA, 5-nonyloxytryptamine binds to the PSA-specific monoclonal antibody 735, enhances neurite outgrowth of cultured primary neurons and process formation of Schwann cells, protects neurons from oxidative stress, reduces migration of astrocytes and enhances myelination in vitro. Furthermore, nonyloxytryptamine treatment enhances expression of the neural cell adhesion molecule (NCAM) and its polysialylated form PSA-NCAM and reduces expression of the microtubuleassociated protein MAP2 in cultured neuroblastoma cells. These results demonstrate that 5-nonyloxytryptamine mimics PSA and triggers PSA-mediated functions, thus contributing to the repertoire of molecules with the potential to improve recovery in acute and chronic injuries of the mammalian peripheral and central nervous systems.

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Fiber scaffolds of polysialic acid via electrospinning for peripheral nerve regeneration

Cited by 16 publications

References 22 publications

Poly(l-lysine) modified zein nanofibrous membranes as efficient scaffold for adhesion, proliferation, and differentiation of neural stem cells

Poly(l-lysine) modified zein nanofibrous membranes as efficient scaffold for adhesion, proliferation, and differentiation of neural stem cells

Neural cell type-specific responses to glycomimetic functionalized collagen

Nonyloxytryptamine mimics polysialic acid and modulates neuronal and glial functions in cell culture

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