In this study, we have studied the effects of temperature and X-ray energy variations on the light output signals from two different fiber-optic sensors, a fiber-optic dosimeter (FOD) based on a BCF-12 as a plastic scintillating fiber (PSF) and a fiber-optic thermometer (FOT) using a silver halide optical fiber as an infrared optical fiber (IR fiber). During X-ray beam irradiation, the scintillating light and IR signals were measured simultaneously using a dosimeter probe of the FOD and a thermometer probe of the FOT. The probes were placed in a beaker with water on the center of a hotplate, under variation of the tube potential of a digital radiography system or the temperature of the water in the beaker. From the experimental results, in the case of the PSF, the scintillator light output at the given tube potential decreased as the temperature increased in the temperature range from 25 to 60 °C. We demonstrated that commonly used BCF-12 has a significant temperature dependence of −0.263 ± 0.028%/°C in the clinical temperature range. Next, in the case of the IR fiber, the intensity of the IR signal was almost uniform at each temperature regardless of the tube potential range from 50 to 150 kVp. Therefore, we also demonstrated that the X-ray beam with an energy range used in diagnostic radiology does not affect the IR signals transmitted via a silver halide optical fiber.