Fibers Obtaining and Characterization Using Poly (Lactic-co-Glycolic Acid) and Poly (Isoprene) Containing Hydroxyapatite and α TCP Calcium Phosphate by Electrospinning Method

Vechietti, Fernanda Albrecht; Marques, Douglas Ramos; Muniz, Nathália Oderich; Santos, Luís Alberto dos

doi:10.4028/www.scientific.net/kem.631.173

Cited by 7 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The choice of these levels was based on results of previous work. 5,18 In all experiments the needle diameter was 0.7 mm and as a collector, a thin aluminum sheet was attached to the fixed collector which was grounded properly.…”

Section: Preparation Of Plga/piepox Membranementioning

confidence: 99%

Electrospun natural rubber latex biocomposite for scaffolds in tissue engineering

Carazzai

Guerra

Henckes

et al. 2021

Journal of Bioactive and Compatible Polymers

View full text Add to dashboard Cite

Fibrous scaffold along with seed cells are essential players for engineered tissue regeneration. Recently, PLGA/epoxidized poly(isoprene) dense membranes have been evaluated for cell growth and have shown satisfactory results. However, porous and fibrous structures suitable for obtaining 3D supports have not yet been evaluated for the PLGA/epoxidized poly(isoprene). The present work aimed to establish the electrospinning conditions for obtaining electrospun membranes with a smaller diameter of fibers and adequate morphology, which were characterized in vitro by their physical, chemical and biological properties. The best electrospun fibers were obtained from the following conditions: an applied voltage of 15 kV, a needle-collector distance of 20 cm and, a flow rate of 5 mL/h. The functional groups of the polymers involved in the blend did not show any changes. The mechanical properties of the electrospun membranes are within the lower limits known to human skin and some soft tissues. The in vitro degradation test showed a loss of mass of approximately 20% in 28 days. Significant adhesion and proliferation of human adipose–derived mesenchymal stem cells were demonstrated, indicating that there was cellular penetration into the scaffold and proliferation. Therefore, the preliminary results suggest that the electrospun PLGA/epoxidized poly(isoprene) membranes have high potential for application as a 3D tissue engineering scaffold.

show abstract

Section: Preparation Of Plga/piepox Membranementioning

confidence: 99%

Electrospun natural rubber latex biocomposite for scaffolds in tissue engineering

Carazzai

Guerra

Henckes

et al. 2021

Journal of Bioactive and Compatible Polymers

View full text Add to dashboard Cite

show abstract

“…2− [32,33]. The peak at 1048 cm −1 can be assigned to the asymmetric stretching (υ 3 PO 4 3− ), while the peak at 1113 cm −1 to the PO 4 3− asymmetric stretching in stoichiometric apatite [34,35]. The band at 1192 cm −1 can be attributed to the asymmetric stretching vibration of HPO 4…”

Section: Ftir Atr Spectroscopymentioning

confidence: 97%

“…The bands at 982 and 2900 cm −1 can be, respectively, linked to the P-O symmetric stretching and (P-)O-H stretching of HPO4 2− [32,33]. The peak at 1048 cm −1 can be assigned to the asymmetric stretching (ʋ3 PO4 3− ), while the peak at 1113 cm −1 to the PO4 3− asymmetric stretching in stoichiometric apatite [34,35]. The band at 1192 cm −1 can be attributed to the asymmetric stretching vibration of HPO4 2− [36], while the one at 870 cm −1 is associated with the ʋ2 symmetric stretching vibrations of carbonate ions, which can be incorporated into the apatites from atmospheric CO2 [32].…”

Section: Ftir Atr Spectroscopymentioning

confidence: 97%

Preserving Intangible Heritage through Tangible Finds: The “Skull with Ears”—St. Luciella ai Librai’s Church (Naples, Italy)

et al. 2023

View full text Add to dashboard Cite

The present study reports the conservative first aid concerning the human cranium known as the “Skull with Ears”, which is conserved in the crypt of Santa Luciella ai Librai’s church in Naples, Italy. These remains have historically been worshipped by devotees within the cult of the “abandoned souls”. The skulls were “adopted” by the Neapolitan population and treated with particular care in exchange for divine favors. The critical preservation status of the “Skull with ears” required a multidisciplinary approach aimed at defining the taphonomy and anthropological features of the cranium, while determining the state of its conservation by using a multi-analytical approach. Multispectral imaging, 3D modeling, X-ray imaging, microscopical observations, and microbiological tests enabled the documentation of the cranium while assessing this state of conservation. Electron scanning microscopy (SEM), energy dispersive spectroscopy (EDS), Fourier-transform infrared spectroscopy (FTIR) in the attenuated total reflectance (ATR) mode, and radiocarbon dating allowed for essential data to be obtained on the cranium’s history and constituent components. The results that were obtained from both the analysis of the cranium and the environmental monitoring of the crypt showed the advanced degradation of the bones due to a significant bacterial attack, which was facilitated by the inadequate environmental conditions at the site of conservation. The acquired data enabled the definition of the most suitable conservation strategy and the securing of the cranium.

show abstract

“…The development of scaffolds using some inorganic particles has attracted a lot of attention, and several techniques have been used to obtain these composites depending on the applications [13,14]. One of these technologies is electrospinning that generates micro/nanofibers on a large scale and at a low cost starting from polymeric solutions [15][16][17][18][19][20]. Although this technique is highly versatile, it presents some limitations as the conductivity of the solution, a high-voltage electric field necessary, and a low production rate.…”

Section: Introductionmentioning

confidence: 99%

Bacteriostatic Behavior of PLA-BaTiO3 Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test

Boschetto

Doan

et al. 2021

Molecules

View full text Add to dashboard Cite

The present work investigated the effect of Polylactic acid (PLA) fibers produced by centrifugal spinning with incorporated BaTiO3 particles to improve their bacteriostatic behavior. The PLA matrix and three composites, presenting three different amounts of fillers, were subjected to UV/O3 treatment monitoring the possible modifications that occurred over time. The morphological and physical properties of the surfaces were characterized by different microscopic techniques, contact angle, and surface potential measurements. Subsequently, the samples were tested in vitro with human dermal fibroblasts (HDF) to verify the cytotoxicity of the substrates. No significant differences between the PLA matrix and composites emerged; the high hydrophobicity of the fibers, derived by the polymer structure, represented an obstacle limiting the fibroblast attachment. Samples underwent bacterial exposure (Staphylococcus epidermidis) for 12 and 24 h. Increasing the concentration of BT, the number of living bacteria and their distribution decreased in comparison with the PLA matrix suggesting an effect of the inorganic filler, which generates a neutralization effect leading to reactive oxygen species (ROS) generation and subsequently to bacterial damages. These results suggest that the barium titanate (BT) fillers clearly improve the antibacterial properties of PLA fibers after aging tests made before bacterial exposure, representing a potential candidate in the creation of composites for medical applications.

show abstract

Fibers Obtaining and Characterization Using Poly (Lactic-co-Glycolic Acid) and Poly (Isoprene) Containing Hydroxyapatite and α TCP Calcium Phosphate by Electrospinning Method

Cited by 7 publications

References 15 publications

Electrospun natural rubber latex biocomposite for scaffolds in tissue engineering

Electrospun natural rubber latex biocomposite for scaffolds in tissue engineering

Preserving Intangible Heritage through Tangible Finds: The “Skull with Ears”—St. Luciella ai Librai’s Church (Naples, Italy)

Bacteriostatic Behavior of PLA-BaTiO3 Composite Fibers Synthesized by Centrifugal Spinning and Subjected to Aging Test

Contact Info

Product

Resources

About