Fibre Number and Sizes of Mouse Soleus Muscle in Early Postnatal Protein Malnutrition

Ihemelandu, E.C.

doi:10.1159/000145949

Cited by 9 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4 These findings emphasize the importance of nutritional recovery at early phases of life, in order to achieve normal growth and development. [13][14][15] Research demonstrates that maternal protein deficiency at the start or from the second half of gestation affects the body weight and number of muscle fibers in the offspring, observed in studies undertaken with low birth weight rats, dogs and monkeys.…”

Section: Histochemical Parameters: Morphology Differentiation and Mementioning

confidence: 89%

See 1 more Smart Citation

Efeito da desnutrição protéica pré e pós-natal sobre a morfologia, a diferenciação e o metabolismo do tecido muscular estriado esquelético em ratos

Alves¹,

Dâmaso²,

Pai³

2008

J. Pediatr. (Rio J.)

View full text Add to dashboard Cite

Objective: To study the contractile properties, metabolism and morphological characteristics of muscles submitted to prenatal and postnatal protein malnutrition.Methods: Animals were distributed into two groups: Control, normoprotein diet (CG; n = 15; 5/5/5), and Malnourished, hypoprotein diet (MG; n = 15; 5/5/5), and examined on the 7th, 14th, and 28th days of the experiment.Total body mass, weight, and the contractile properties and morphology of the anterior tibial muscle were assessed.Several 8 µm-thick tissue samples were taken from 7, 14, and 28 day old rats and stained with HE or subjected to NADH-TR or m-ATPase (pH = 4.4) techniques.Results: Body and muscle weight were lower in the malnourished group. On the 7th day of malnutrition, muscle samples exhibited fibers with smaller diameter, higher polymorphism and higher endomysial conjunctive tissue content.Histochemical methods were unable to precisely determine the types of fiber present. On the 14th day, there were smaller muscle fibers, more polymorphism, many of them with central nuclei and moderate endomysial conjunctive tissue content. With reference to contractile properties, the m-ATPase reaction identified both slow and fast fibers. The NADH-TR reaction revealed the following types of fiber: slow oxidative (SO), fast oxidative glycolytic (FOG) and fast glycolytic (FG). On the 28th day smaller, bunched muscle fibers varying shapes. All three types of fiber exhibited unclear recognition limits with respect to contraction and metabolism. Conclusion:Our experimental results suggest that, in addition to the reduction in numbers of fibers, malnutrition retards the differentiation of the morphological, metabolic, and contractile characteristics of skeletal muscle fibers in growing rats.J Pediatr (Rio J). 2008;84(3):264-271: Malnutrition, skeletal muscle.

show abstract

Section: Histochemical Parameters: Morphology Differentiation and Mementioning

confidence: 89%

“…Similar results have been reported by several other authors in their studies of prenatal malnutrition. 4 It has also been observed that protein malnutrition statistically reduces the muscle tissue weight. This is probably due to lost tissue proteins.…”

Section: 516mentioning

confidence: 99%

Efeito da desnutrição protéica pré e pós-natal sobre a morfologia, a diferenciação e o metabolismo do tecido muscular estriado esquelético em ratos

Alves¹,

Dâmaso²,

Pai³

2008

J. Pediatr. (Rio J.)

View full text Add to dashboard Cite

show abstract

“…Feed restriction significantly affects the growth and differentiation of cells, and the earlier the malnutrition, the greater the impairment in tissues and organs (Ihemelandu, 1985). Studies in rats, dogs and monkeys showed that protein restriction during pregnancy affects body weight and the number of muscle fibers of offspring (Nascimento et al, 1990;Oliveira et al, 1999;Nunes et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The number of undernourished people in the world is extremely high and reached almost 1 billion in 2010 (FAO, 2010). Malnutrition in developing countries has mostly been attributed to a low protein diet (Ihemelandu, 1985;Ge and Chang, 2001) or a low quality of food consumed (Morgane et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of skeletal muscle regeneration in experimental model after malnutrition

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to analyze muscle regeneration after cryoinjury in the tibialis anterior muscle of young rats that were malnourished and then recovered. Forty Wistar rats were divided into a nourished group that received a normal protein diet (14% casein) for 90 days and a malnourished and recovered rats group (MR) that was submitted to 45 days of malnutrition with a hypoproteic diet (6% casein) followed by 45 days of a normal protein diet (14% casein). After the recovery period, all of the animals underwent cryoinjury in the right tibialis anterior muscle and euthanasia after 7, 14 and 21 days. The amount of connective tissue and the inflammation area was higher in the malnutrition recovered injury MR group (MRI) at 14 days post-injury (p < 0.05). Additionally, the cross-sectional area (CSA) of the regenerated fibers was decreased in the MRI (p < 0.05). The MyoD and myogenin protein levels were higher in the nourished injury group. Similar levels of TGF-β1 were found between groups. The proposed malnutrition protocol was effective in showing delayed changes in the regeneration process of the tibialis anterior muscle of young rats. Furthermore, we observed a delay in muscle repair even after nutritional recovery.Keywords: skeletal muscle, protein malnutrition, cryolesion, myogenic regulatory factors, muscle healing process. Avaliação da regeneração do músculo esquelético em modelo experimental após desnutriçãoResumo O objetivo do presente estudo foi analisar a regeneração muscular após criolesão no músculo tibial anterior de ratos jovens desnutridos e recuperados. Foram utilizados 40 ratos da linhagem Wistar, divididos em 2 grupos: ratos nutridos receberam dieta normoproteica (14% de caseína) por 90 dias; e ratos desnutridos e recuperado submetidos a duas fases nutricionais pós-desmame, correspondendo a 45 dias de desnutrição com dieta hipoproteica (6% caseína), seguida por 45 dias de dieta normoproteica (14% caseína). Ao completar a fase de recuperação, todos os animais foram submetidos à criolesão no músculo tibial anterior direito e a eutanasia ocorreu 7, 14 e 21 dias após a lesão. A quantidade de tecido conjuntivo e a área de inflamação 14 dias pós-lesão foi maior no grupo desnutrido, recuperado e lesado (MRI -malnourished, recovered and injured group) (p < 0,05). A área de secção transversa (AST) das fibras regeneradas do grupo MRI foi menor (p < 0,05). O conteúdo das proteínas MyoD e Miogenina foi maior no grupo nutridos e lesados. A citocina TGF-β1 não apresentou diferença entre os grupos. O protocolo proposto foi eficaz para demonstrar alterações no processo de regeneração do músculo tibial anterior de ratos jovens, atrasando o reparo muscular mesmo após a recuperação nutricional.Palavras-chave: músculo esquelético, desnutrição proteica, criolesão, fatores de regulação miogênica, processo de recuperação do músculo.

show abstract

Long-term effects of the administration of the convulsive substance DL-methionine-DL-sulfoximine to the rabbit

Apostolakis

Anogianakis

Kallaras

et al. 1989

Brain Research Bulletin

View full text Add to dashboard Cite

Fibre Number and Sizes of Mouse Soleus Muscle in Early Postnatal Protein Malnutrition

Cited by 9 publications

References 12 publications

Efeito da desnutrição protéica pré e pós-natal sobre a morfologia, a diferenciação e o metabolismo do tecido muscular estriado esquelético em ratos

Efeito da desnutrição protéica pré e pós-natal sobre a morfologia, a diferenciação e o metabolismo do tecido muscular estriado esquelético em ratos

Evaluation of skeletal muscle regeneration in experimental model after malnutrition

Long-term effects of the administration of the convulsive substance DL-methionine-DL-sulfoximine to the rabbit

Contact Info

Product

Resources

About