Fibre type‐specific satellite cell content in two models of muscle disease

Abstract

The capacity to generate new SCs is increased even in severely impaired muscles and a fibre type-specific enhancement of SC occurs in type I muscle fibres in DMD.

Fiber type‐specific response of skeletal muscle satellite cells to high‐intensity resistance training in dialysis patients

“…44,45 There are also reports of lower type II fiber SC content when compared with type I fibers in the trapezius muscle of women with trapezius myalgia 43 and in the paravertebral muscle of Duchenne muscular dystrophy patients. 46 Investigations of SC content of type I and II fibers in young, healthy individuals appear to agree on an equal distribution, 47 with the divergence beginning at around age 50 years, and with type II fiber size also showing a decrease. 48 In our study, the statistical difference in SC content between type I and II fibers disappeared once the data for SC content were normalized for fiber area.…”

Satellite cells from dystrophic muscle retain regenerative capacity

“…This difference could be due to the genetic background and/or on the sex of the mice analyzed, as male and female adult mouse EDL myofibers bear different number of satellite cells ( Neal et al, 2012 ). However, satellite cell number is also elevated in DMD patients relative to controls ( Bankole et al, 2013; Kottlors and Kirschner, 2010 ). Unlike our findings in mdx , reduction of satellite cell number is a hallmark of some other mouse models of muscular dystrophies, e.g.…”

Satellite cells in human skeletal muscle plasticity

“…In animal studies, the Pax7 antigen is the most widely used marker to identify satellite cells (Olguin and Olwin, 2004 ; Kuang et al, 2006 ; Relaix et al, 2006 ; Zammit et al, 2006 ; Day et al, 2007 ; Ishido et al, 2009 ; Lepper et al, 2011 ; McCarthy et al, 2011 ; Murphy et al, 2011 ; Sambasivan et al, 2011 ; Jackson et al, 2012 ) and has been proposed to be the most reliable marker for satellite cells in mouse muscle (Kuang and Rudnicki, 2008 ; Zammit, 2008 ). In the past decade, an increasing number of human studies have utilized the Pax7 antibody for the identification of skeletal muscle satellite cells in vivo (McLoon and Wirtschafter, 2003 ; Reimann et al, 2004 ; Verdijk et al, 2007 ; McKay et al, 2008 ; Mackey et al, 2009 ; McKay et al, 2009 ; Kottlors and Kirschner, 2010 ; McKay et al, 2010 ; Mackey et al, 2011a ; Toth et al, 2011 ; McKay et al, 2012 ; Menon et al, 2012 ; Nielsen et al, 2012 ; Theriault et al, 2012 ; Walker et al, 2012 ; Bankole et al, 2013 ; Cermak et al, 2013 ; Delhaas et al, 2013 ; Joanisse et al, 2013 ; McKay et al, 2013 ; Suetta et al, 2013 ; Bellamy et al, 2014 ; Farup et al, 2014a , b ; Fry et al, 2014a ; Grubb et al, 2014 ; Kern et al, 2014 ; Mackey et al, 2014 ; Snijders et al, 2014b , c ; Theriault et al, 2014 ). Reimann et al ( 2004 ) compared M-Cad and Pax7 in biopsies taken from healthy and pathological human muscle.…”

“…Furthermore, recent studies have emphasized the need for muscle fiber type specific analyses of satellite cell number. Resting satellite cell content has been shown to vary between muscle fiber types in both healthy and diseased populations (Verdijk et al, 2007 , 2009 , 2012 ; Verney et al, 2008 ; Snijders et al, 2011 , 2014c ; McKay et al, 2012 ; Suetta et al, 2012 ; Bankole et al, 2013 ). Furthermore, satellite cells have been shown to respond differently during post-exercise recovery depending on the muscle fiber type in which they are located (McKay et al, 2012 ; Cermak et al, 2013 ; Joanisse et al, 2013 ; Fry et al, 2014a ; Snijders et al, 2014c ).…”