Fibrin-Based Microsphere Reservoirs for Delivery of Neurotrophic Factors to the Brain

Samal, Juhi; Hoban, Deirdre B.; Naughton, Carol; Concannon, Ruth M.; Dowd, Eilís; Pandit, Abhay

doi:10.2217/nnm.14.221

Cited by 32 publications

(3 citation statements)

References 42 publications

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“…PDA-Fib microcapsules were obtained by etching the PS cores from the PS@PDA-Fib microparticles with THF. 26 After stirring at room temperature for 24 h, the solution was centrifuged at 3000 rpm and washed with water and ethanol at least three times to remove the residual solvent. Lastly, the microparticles were dried in a vacuum oven overnight at 30 °C.…”

Section: Methodsmentioning

confidence: 99%

Bio-inspired microcapsule for targeted antithrombotic drug delivery

Wang

et al. 2018

RSC Adv.

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Section: Methodsmentioning

confidence: 99%

Bio-inspired microcapsule for targeted antithrombotic drug delivery

Wang

et al. 2018

RSC Adv.

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“…[138] Choi et al have also tested the effect of TGF-β-1 bound via molecular affinity to chitosan scaffolds, showing that MSCs and chondrocytes increased their GAG deposition and formed neocartilage in vivo in the presence of this growth factor. [142] Neural growth factors (NGFs) have also been integrated with biomaterials, especially for nervous TE strategies, primarily included in microspheres, alone for controlled brain delivery [143] or within tissue engineering scaffolds containing the NGFreleasing hydrogel spheres such as reported for silk fibroin ones [144] applied to spinal cord regeneration. Even though these approaches focus on the release of soluble NGF, researchers have also devised strategies for directly functionalizing scaffolds with NGF, such as conduits envisaging peripheral nerve regeneration, [145] which may enhance biological function due to NGF immobilization and ECM-like presentation.…”

Section: Growth-factor-based Approachesmentioning

confidence: 99%

Pushing the Natural Frontier: Progress on the Integration of Biomaterial Cues toward Combinatorial Biofabrication and Tissue Engineering

2022

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and shown able to integrate the organism and maintain physiological function for many years after implantation. Even though these are examples of success, they represent mostly nonvital organs generally simpler in terms of morphology and cellular complexity. On the other hand, vital organs like the kidney, heart, liver, or lungs top the list of transplant requirements worldwide. [4] Yet, there are no TE equivalents of these organs so far, resulting from their complex cellular function and architecture, which are extremely hard to engineer, leading to a continuous effort to better organ recovery and transplantation. [5] Biomaterials have been faithful companions of cells in TE strategies, physically supporting them and allowing the maintenance of their phenotype in distinct environments. In fact, with the recent advances in the field of 3D printing, biomaterial-based 3D structures can now be manipulated to approach the architecture of complex tissues and organs, such as vascular beds and cardiac components. [6,7] However, shape and architecture alone cannot assure the ultimate TE challenge: recapitulation of physiological cellular and tissue function. As such, there is one additional burden for biomaterials to carry-the control of cellular behavior. These requirements force upon biomaterials the need to be intelligent and dynamic, supporting and efficiently directing the behavior of cells toward specific outcomes.Natural-origin materials have been continuously derived from different animal and plant components, gathering attention as biomaterials due to their original role in biological environments. [8] However, their bioactivity and biodegradability can be just as limited as that of synthetic components. Thus, this review explores some of the leading natural materials that have been used in TE approaches, looking at their typical applications, degradability properties, and ability to interact with and be modified by the action of cells-a biomimetic, extracellular matrix (ECM)-like behavior. Furthermore, we explore the progress on the use of adhesive sequences and growth factors and their combinatorial applications with emerging synergistic results and novel biological outcomes. Likewise, as force and shape establish themselves as fair opponents to classical biochemical cues, [9,10] we review the recent progress in manipulating stiffness and topography within 3D natural materials and how they can further boost cellular responses.The engineering of fully functional, biological-like tissues requires biomaterials to direct cellular events to a near-native, 3D niche extent. Natural biomaterials are generally seen as a safe option for cell support, but their biocompatibility and biodegradability can be just as limited as their bioactive/ biomimetic performance. Furthermore, integrating different biomaterial cues and their final impact on cellular behavior is a complex equation where the outcome might be very different from the sum of individual parts. This review critically analyses recent progress on biomaterial-indu...

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“…In this study, we used conducting AAM (CAAM) substrates as nerveinterfacing membranes. We also report the first attempt to dope nanoporous membranes with NGF, which has been shown to induce neural differentiation in pheochromocytoma-originated PC 12 cells [30,31], the model cell line used in this study. CAAMs with 100 and 250 nm pore diameters were fabricated by coating a thin layer of C on AAMs as described previously [32].…”

Section: Introductionmentioning

confidence: 99%

Protein-releasing conductive anodized alumina membranes for nerve-interface materials

Altuntaş

Büyükserin

Haider

et al. 2016

Materials Science and Engineering: C

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Nanoporous anodized alumina membranes (AAMs) have numerous biomedical applications spanning from biosensors to controlled drug delivery and implant coatings. Although the use of AAM as an alternative bone implant surface has been successful, its potential as a neural implant coating remains unclear. Here, we introduce conductive and nerve growth factor-releasing AAM substrates that not only provide the native nanoporous morphology for cell adhesion, but also induce neural differentiation. We recently reported the fabrication of such conductive membranes by coating AAMs with a thin C layer. In this study, we investigated the influence of electrical stimulus, surface topography, and chemistry on cell adhesion, neurite extension, and density by using PC 12 pheochromocytoma cells in a custom-made glass microwell setup. The conductive AAMs showed enhanced neurite extension and generation with the electrical stimulus, but cell adhesion on these substrates was poorer compared to the naked AAMs. The latter nanoporous material presents chemical and topographical features for superior neuronal cell adhesion, but, more importantly, when loaded with nerve growth factor, it can provide neurite extension similar to an electrically stimulated CAAM counterpart.

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Fibrin-Based Microsphere Reservoirs for Delivery of Neurotrophic Factors to the Brain

Abstract: Fibrin hollow microspheres act as a suitable delivery platform for neurotrophic factors with tunable loading efficiency and maintaining their bioactive form after release in vivo.

Cited by 32 publications

References 42 publications

Bio-inspired microcapsule for targeted antithrombotic drug delivery

Bio-inspired microcapsule for targeted antithrombotic drug delivery

Pushing the Natural Frontier: Progress on the Integration of Biomaterial Cues toward Combinatorial Biofabrication and Tissue Engineering

Protein-releasing conductive anodized alumina membranes for nerve-interface materials

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