Fibrin-Based Tissue Engineering: Comparison of Different Methods of Autologous Fibrinogen Isolation

Dietrich, Maren; Heselhaus, Johanna; Wozniak, Justyna; Weinandy, Stefan; Mela, Petra; Tschoeke, Beate; Schmitz‐Rode, Thomas; Jockenhoevel, Stefan

doi:10.1089/ten.tec.2011.0473

Cited by 60 publications

(42 citation statements)

References 28 publications

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“…Isolation techniques (eg, cryoprecipitation and ethanol precipitation), batch to batch variability, and composition (eg, concentration of thrombin, fibrinogen, factor XIII, and antifibrolytics) lead to different mechanical and biological properties of the generated clots. 16,17 For example, Dickneite et al showed clear differences in hemostatic and clot strength properties of 12 commercially available fibrin sealants. 17 Furthermore, differences in component origin may change the biochemistry of the clot, like interspecies or pooled and single donor.…”

Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

“…These include cryoprecipitation or chemical precipitation with ethanol, ammonium sulfate, or poly(ethylene glycol). 16,90 The principal advantage of cryoprecipitation over other procedures is that no potentially cytotoxic chemicals are required for this process; however, it is time-consuming and the fibrinogen yield is extremely low. Cryoprecipitation is time-consuming but chemical precipitation is rapid.…”

Section: Fibrin Preparationmentioning

confidence: 99%

“…However, purity of the final product is a concern. 16,90 There are also methods and devices to generate thrombin from a single donation of plasma. 87,91 Several considerations must be taken in account when selecting the appropriate fibrin glue for scaffold fabrication (Table 3).…”

Section: Fibrin Preparationmentioning

confidence: 99%

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A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

Section: Fibrin Preparationmentioning

confidence: 99%

See 1 more Smart Citation

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

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show abstract

“…The test was performed according to a standard protocol described elsewhere. 18 Briefly, HFFs or HUVECs were cultured in a 96-well plate at a density of 1 · 10 4 cells/well in standard medium (DMEM and 10% FBS for HFFs; EGM-2 for HUVECs). After 48 h of preculturing, medium was replaced with test medium (appropriate medium without FBS, preincubated with nonfunctionalized and biofunctionalized PLLA for 72 h at 37°C).…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Biofunctionalized Microfiber-Assisted Formation of Intrinsic Three-Dimensional Capillary-Like Structures

Weinandy

Laffar

Unger

et al. 2014

Tissue Engineering Part A

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“…Fibrin is an attractive scaffold material because of its autologous origin, rapid polymerization, the tunable degradation using protease inhibitors, 20 the autologous release of growth factors, 21 and the manufacturability into complex three-dimensional (3D) geometries with homogeneous cell seeding. 22 However, being a hydrogel, fibrin is mechanically weak and TEHVs based on fibrin could only be implanted in the low-pressure pulmonary circulation of animal models.…”

Section: Introductionmentioning

confidence: 99%