Fibrin‐chitosan composite substrate for <i>in vitro</i> culture of chondrocytes

Gamboa-MartÍnez, T. C.; Cruz, Dunia M. García; Cardá, Carmen; Ribelles, J.L. Gómez; Ferrer, Gloria Gallego

doi:10.1002/jbm.a.34330

Cited by 4 publications

(3 citation statements)

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“…Human chondrocytes cultured on the composite substrate were viable during the culture period (28 days): at the end the composite substrate showed 41% more collagen-II and 13% higher production of sulfated glycosaminoglycans with respect to the amounts found at 14 days. The de-differentiated chondrocytes cultured in monolayer on the composite could re-acquire characteristics of differentiated cells without using three-dimensional substrates or chondrogenic media [ 91 ].…”

Section: Genipin-crosslinked Collagen/gelatin For the Regenerationmentioning

confidence: 99%

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

et al. 2015

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The present review article intends to direct attention to the technological advances made since 2009 in the area of genipin-crosslinked chitosan (GEN-chitosan) hydrogels. After a concise introduction on the well recognized characteristics of medical grade chitosan and food grade genipin, the properties of GEN-chitosan obtained with a safe, spontaneous and irreversible chemical reaction, and the quality assessment of the gels are reviewed. The antibacterial activity of GEN-chitosan has been well assessed in the treatment of gastric infections supported by Helicobacter pylori. Therapies based on chitosan alginate crosslinked with genipin include stem cell transplantation, and development of contraction free biomaterials suitable for cartilage engineering. Collagen, gelatin and other proteins have been associated to said hydrogels in view of the regeneration of the cartilage. Viability and proliferation of fibroblasts were impressively enhanced upon addition of poly-l-lysine. The modulation of the osteocytes has been achieved in various ways by applying advanced technologies such as 3D-plotting and electrospinning of biomimetic scaffolds, with optional addition of nano hydroxyapatite to the formulations. A wealth of biotechnological advances and know-how has permitted reaching outstanding results in crucial areas such as cranio-facial surgery, orthopedics and dentistry. It is mandatory to use scaffolds fully characterized in terms of porosity, pore size, swelling, wettability, compressive strength, and degree of acetylation, if the osteogenic differentiation of human mesenchymal stem cells is sought: in fact, the novel characteristics imparted by GEN-chitosan must be simultaneously of physico-chemical and cytological nature. Owing to their high standard, the scientific publications dated 2010–2015 have met the expectations of an interdisciplinary audience.

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Section: Genipin-crosslinked Collagen/gelatin For the Regenerationmentioning

confidence: 99%

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

et al. 2015

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“…Chitosan was chosen as a biomaterial because of its biocompatibility, biodegradability, and nontoxicity [18,19]. It can be used as an individual compound in a carrier [13,14] or in combination with other polymers (fibrin, gelatin, collagen, and heparin) [20][21][22][23][24] and inorganic substances [15,16]. Ch is a biodegradable cationic polysaccharide composed of (1 → 4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit) which is known to accelerate wound healing and bone formation [15].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Microcrystalline Chitosan and Fibrin Membranes as Platelet-Derived Growth Factor-BB Carriers with Amoxicillin

Bodek

Nowak

Kozakiewicz

et al. 2015

International Journal of Polymer Science

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The aim of this study was to describe the mechanical and sorption features of homogeneous and composite membranes which consist of microcrystalline chitosan (MCCh) and fibrin (Fb) in various proportions as well as thein vitrokinetics of platelet-derived growth factor-BB (PDGF-BB) released from ten types of membranes in the presence or absence of amoxicillin (Am). The films were characterized by Fourier transform infrared (FTIR) spectroscopy, mechanical tests: breaking strength (Bs) and elongation at break (Eb), as well as SEM images, and swelling study. The influence of the form of samples (dry or wet) on Young’s modulus (E) was also examined. The homogeneous MCCh (M1) and composite M3 and M4 (MCCh : Fb = 2 : 1 and 1 : 1) membranes were characterized by good sorption properties and higher mechanical strength, when compared with Fb (M2) membrane. Connecting MCCh with Fb decreases release of PDGF-BB and increases release of Am. The most efficient release of PDGF-BB was observed in the case of M4 (the optimum MCCh : Fb ratio was 1 : 1) membrane. It was found that the degree of PDGF-BB release from the membrane is influenced by the physicochemical and mechanical characteristics of the films and by its affinity to growth factor PDGF-BB.

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“…Tri-copolymers of gelatin with CS and HA [75]- [78], with chitosan and HA [79], and with chitosan and agarose [80] have also been studied. [83].…”

Section: Protein-polysaccharide Hydrogelsmentioning

confidence: 99%

Protein-based injectable hydrogels towards the regeneration of articular cartilage

Poveda-Reyes¹

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A mis seres queridos, con ellos todo es posible "The future belongs to those who believe in the beauty of their dreams"Eleanor Roosevelt Acknowledgements 7 AcknowledgementsAfter reaching this point I would like to thank everybody that in one way or another has helped me through these four years of work.First of all, I gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the DPI2010-20399-C04-03 and MAT2013-46467-C4-1-R projects, my FPI fellowship BES-2011-046144 and the EEBB-I-13-06179 and EEBB-I-14-08725 research stay grants.I would especially like to thank my thesis supervisor, Dr. Gloria Gallego Ferrer, for helping me in everything I needed through these four years, for giving me this opportunity and for all her efforts in my assistance. Also thanks to José Luis Gómez Ribelles for his valuable help and brilliant ideas.Thanks to the staff of the Microscopy Service at the Universitat Politècnica de València, for their guidance and help during the SEM sessions.I would also like to express my gratitude to my supervisors during my stays at other Research Centers. Thanks to Prof. Ulrica Edlund, who during my stay at the Fiber and Polymer Technology Department (KTH) in Stockholm, helped me with everything I could need and to all the people in the center (Laleh, Anas, Soheil, Bekir, Parmida, Robertus, Arturo, etc.) that helped me in the lab and made my stay easier. Thanks to Prof. Manuel Salmerón Sánchez at the Microenvironments for Medicine group (MiMe) (University of Glasgow) for giving me the opportunity to learn the basics of cell culture in his lab, his guidance and valuable help. I would also like to thank Dr. Vladimira Moulisova for teaching me everything I know of cell biology work and being so patient and friendly. And to all the people of the group (Marco, Sara, Eleni, Mark, Alex, Jake, Elie, Frankie, etc.), as they say "People make Glasgow" and they made me feel at home. I cannot forget all the people at the Center for Biomaterials and Tissue Engineering: to Laura Teruel, for being so patient with everyone, so helpful and keeping the lab in a perfect state, and all the lecturers for their help and advice, especially to Ana Vallés and Manuel Monleón, who introduced me to the wonderful field of Tissue Engineering.I would like to especially thank Tatiana C. Gamboa, who guided me in my first years in the lab. To Suhail, Rocío, Marta, Félix, Petra, Leonardo, Rebeca and Esther, I helped them in their first steps in the lab but I learnt a lot from them and they helped me a lot, both professional and personally. Also thanks to Alex Rodrigo who helped me a lot in my first cell cultures even if he had a million things to do.Thanks to all the people who have been in the "Sala de Reuniones" office: Manu, María, Line, Amparo G, Roberto, Guillermo, Álvaro, Laia, Rubén, etc., thanks for the break times, your laughs, your help and being the way you are.Special thanks to my friends, they gave me support through all these years, with them life is better.Finally, I w...

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Fibrin‐chitosan composite substrate for in vitro culture of chondrocytes

Cited by 4 publications

References 39 publications

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

Evaluation of Microcrystalline Chitosan and Fibrin Membranes as Platelet-Derived Growth Factor-BB Carriers with Amoxicillin

Protein-based injectable hydrogels towards the regeneration of articular cartilage

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