Fibrin glue does not assist migration and proliferation of chondrocytes in collagenic membranes: an in vitro study

Abstract

Background Some authors secured the membrane during matrix-induced autologous chondrocyte implantation (mACI) with fibrin glue or did not use a formal fixation. The real impact of fibrin glue addition on chondrocytes migration and proliferation has not yet been clarified. This study evaluated the impact of fibrin glue on a chondrocyte loaded collagenic membrane. Methods A resorbable collagen I/III porcine derived membrane commonly employed in AMIC was used for all experiments. Chondrocytes from three difference donors were used. At 1-, 2-, 3-, 4-, 6-, and at 8-week the membranes were embedded in Mounting Medium with Dapi (ABCAM, Cambridge, UK). The Dapi contained in the mounting medium ties the DNA of the cell nucleus and emits a blue fluorescence. In this way, the spreading of the cells in the membrane can be easily monitored. The outcomes of interest were to evaluate (1) cell migration and (2) cell proliferation within the porous membrane layer. DAPI/nuclei signals were analysed with fluorescence microscope under a magnification of 100-fold. Results The no-fibrin group demonstrated greater migration of the cells within the membrane. Although migration resulted higher in the no-fibrin group at every follow-up, this difference was significant only at week 1 (P < 0.001), 2 (P = 0.004), and 3 (P = 0.03). No difference was found at week 3, 6, and 8. The no-fibrin group demonstrated greater proliferation of the chondrocytes within the membrane. These differences were significant at week 4 (P < 0.0001), 6 (P < 0.0001), 8 (P < 0.0001). Conclusion The use of fibrin glue over a resorbable membrane leads to lower in vitro proliferation and migration of chondrocytes.

Modified Low-Flow Arthroscopic Sandwich Technique for Complex Osteochondral Lesions of the Talus

“…The putty-like consistency of the viable allograft naturally adheres to the donor site, eliminating the need for fibrin glue, which can lower in vitro proliferation and migration of chondrocytes. 16 Eliminating fibrin glue removes a cellular barrier, additional cost, and surgeon frustration given the complexity of applying a thin, even layer and its associated drying time.…”

Autologous chondrocyte implantation in the knee is effective in skeletally immature patients: a systematic review

“…Moreover, the membrane was sutured in the first two generations of ACI (pACI and cACI) [ 33 ]. Although suturing allows a more stable fixation of the membrane, it produces partial-thickness lesions of the articular cartilage which may not heal and may enlarge with time, leading to pain, and premature osteoarthritis [ 21 , 43 ]. Compared to the previous generations, mACI allows less invasive approaches (mini-arthrotomy or arthroscopy), avoids graft suture, and allows shorter surgical time [ 39 ].…”

Fibrin glue does not promote migration and proliferation of bone marrow derived mesenchymal stem cells in collagenic membranes: an in vitro study

“…Informed consent was obtained from all subjects and/or their legal guardian(s). The methods used in the present study were already published in a previous in vitro study which evaluated the impact of fibrin glue on a chondrocyte loaded collagenic membrane 23 . Briefly, a resorbable collagen I/III porcine derived membrane (Cartimaix-collagen-membrane; Matricel GmbH, Germany) commonly employed in AMIC was used for the experiments.…”