Fibrin in Reproductive Tissue Engineering: A Review on Its Application as a Biomaterial for Fertility Preservation

Chiti, Maria Costanza; Dolmans, Marie-Madeleine; Donnez, Jacques; Amorim, Christiani Andrade

doi:10.1007/s10439-017-1817-5

Cited by 68 publications

(48 citation statements)

References 80 publications

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“…Stem cells can be derived from different sources; however, different types of stem cells are still hard to gain. Preclinical studies have reported that transplantation of stem cells into POF animal models restored ovarian function and generated immature oocytes (Chiti, Dolmans, Donnez, & Amorim, 2017).…”

Section: Tissue Engineering and Pofmentioning

confidence: 99%

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Premature ovarian failure and tissue engineering

Ghahremani-Nasab

Ghanbari

Jahanbani

et al. 2019

Journal Cellular Physiology

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Section: Tissue Engineering and Pofmentioning

confidence: 99%

“…Reproductive technology has been recently used in tissue engineering and regenerative medicine techniques to restore POF-related infertility by overcoming the donor supply limitations and immune system complications for tissue transplantation (Chiti et al, 2017;Volkova, Yukhta, & Goltsev, 2017).…”

Section: Tissue Engineering and Pofmentioning

confidence: 99%

Premature ovarian failure and tissue engineering

Ghahremani-Nasab

Ghanbari

Jahanbani

et al. 2019

Journal Cellular Physiology

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“…It releases steroid hormones after puberty and thereby fosters follicular development and periodic oocyte release (Y. J. Kim et al, ; Y. J. Kim, Ku, Rosenwaks et al, ; Y. J. Kim, K. E. Park et al, ). Ideally and anatomically, the artificial ovary needs to contain follicles isolated from cryopreserved ovarian tissue and possibly other ovarian cells to provide growth factors (Chiti et al, ). It also requires a proper delivery scaffold that is biocompatible, minimally inflammatory, suitable for neo‐angiogenesis, and degradable after engraftment for not to disturb follicular growth and migration (Amorim & Shikanov, ).…”

Section: Theoretical Overview Of the Artificial Ovarymentioning

confidence: 99%

“…Many researchers have described the essential components and characteristics of the artificial ovary. A group of investigators maintained that the artificial ovary refers to “a temporary surrogate of the natural ovary in which isolated follicles, ovarian stromal cells and a combination of growth factors can be encapsulated together inside a biomaterial‐based scaffold” (Chiti, Dolmans, Donnez, & Amorim, ). Another group suggested that the artificial ovary contain isolated follicles in a biomaterial and thus is able to preserve fertility and replace hormonal functions of the ovary with a significantly decreased risk of introducing malignant cells back into patients (Soares et al, ).…”

Section: Introductionmentioning

confidence: 99%

A new possibility in fertility preservation: The artificial ovary

Cho

Kim

Noh

et al. 2019

J Tissue Eng Regen Med

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Conventional fertility preservation methods such as oocyte or embryo cryopreservation are currently insufficient to treat including those patients with prepubertal cancer and premature ovarian failure. Ovarian tissue cryopreservation presents as an alternative but has limitations with a potential risk of reintroducing malignant cells in patients who recover from cancer, those of chemotherapy prior to tissue cryopreservation. The so called “artificial ovary” aims to resolve this issue by transplanting isolated follicles with or without a biological scaffold. The artificial ovary may also offer an effective alternative option for those who cannot benefit from traditional assisted reproductive techniques such as in vitro fertilisation. To date, in animal studies and human trial, the artificial ovary restored endocrine function, achieved in vivo follicular development, and resulted in successful pregnancies. However, development of a technique for higher follicular recovery rate and a more optimised design of delivery scaffold, better transplantation techniques to prevent postsurgical ischemia, and consideration for genetic safety are required for safer and consistent human clinical applications. Ideas from different transplantation surgeries (e.g., entire ovary, ovarian cortex, and transplantation with tissue‐engineered products) can be applied to enhance the efficacy of artificial ovarian transplantation. For the better application of artificial ovary, a deeper understanding of mechanical and biochemical properties of the ovary and folliculogenesis after cryopreservation, transplantation with or without scaffold, and development of sophisticated in vivo imaging techniques of transplanted artificial ovary need to precede its efficient clinical application.

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“…In order to develop a suitable supporting matrix for human isolated follicles, different polymers have been used in our in vivo studies (Dolmans et al, 2007;Paulini et al, 2016;Chiti et al, 2018). Among all these studies, fibrin formulations with high concentrations of fibrinogen and thrombin were found to be the best choice for supporting follicle survival after xenotransplantation (Paulini et al, 2016;Chiti et al, 2017aChiti et al, , 2018. This is probably because their ultrastructure and rigidity resemble the human ovarian cortex (Paulini et al, 2016;Chiti et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of the human ovarian cell population for transplantation into an artificial ovary

Shahri¹,

Chiti²,

Amorim³

2019

Anim Reprod

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To support survival and growth of follicles, the transplantable artificial ovary should mimic the original organ, offering a physical (3D matrix) and biological support (cells). In order to replicate the ovarian cell populations, the aim of this study is to assess the proportions of stromal and endothelial cells in the ovarian cortex. To this end, ovarian biopsies were obtained from six women (mean age: 49 years). The epithelial layer and medulla were carefully removed. The cortex was finely minced and enzymatically digested and the isolated cells were fixed. For cell characterization, immunostaining for CD31 (for endothelial cells) and inhibin-α (for granulosa cells) was performed. Positive cells in each staining were counted and the proportion of the different cell populations was estimated from the total number of isolated cells. Since there is no specific marker for ovarian stromal cells, we estimated the proportion of these cells by performing a vimentin immunostaining and subtracting the proportions of CD31-and inhibin-α-positive cells. Immunostaining showed that 84% of isolated cells were vimentin-positive. From this pool, 3% were endothelial cells and 1% granulosa cells. Consequently, the population of ovarian stromal cells was 80%. In conclusion, our findings show that stromal cells represent the larger population of cells in the human ovarian cortex. While this ensures follicle survival and development in a normal ovary, we believe that the low proportion of endothelial cells could have a negative impact on the angiogenesis in the artificial ovary after the first days of transplantation.

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Fibrin in Reproductive Tissue Engineering: A Review on Its Application as a Biomaterial for Fertility Preservation

Cited by 68 publications

References 80 publications

Premature ovarian failure and tissue engineering

Premature ovarian failure and tissue engineering

A new possibility in fertility preservation: The artificial ovary

Isolation and characterization of the human ovarian cell population for transplantation into an artificial ovary

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