Fibrin Microbeads for Isolating and Growing Bone Marrow–Derived Progenitor Cells Capable of Forming Bone Tissue

Gurevich, O. A.; Vexler, Akiva; Marx, Gerard; Prigozhina, Tatyana B.; Levdansky, Lila; Slavin, Shimon; Shimeliovich, Irina; Gorodetsky, Raphael

doi:10.1089/107632702760240571

Cited by 75 publications

(56 citation statements)

References 19 publications

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“…97 In a typical procedure, FMBs are synthesized by oil-stirring of a fibrinogen and thrombin mixture at 65°C-75°C for 6-8 hours and stored as a dry powder. 98 At this high temperature, fibrinogen denatures while factor XIIIa can remain active and cross-link proteins. Due to the conformational transformations of fibrinogen at these high temperatures, FMBs are highly haptotactic to various cell types, including osteogenic bone marrow-derived progenitors.…”

Section: Different Forms Of Fibrin Applications In Bone Tissue Enginementioning

confidence: 99%

“…Due to the conformational transformations of fibrinogen at these high temperatures, FMBs are highly haptotactic to various cell types, including osteogenic bone marrow-derived progenitors. 99,100 For bone tissue engineering studies, the obtained FMBs were incubated for 2 days in a suspension containing whole 97,98,101 Next, authors cultured FMB-MSCs constructs in osteogenic medium and obtained a bone-like structure which was implanted into a mouse skull defect. After 2 months, results showed that defects filled with bone-like tissue had a Ca/P ratio similar to that of the native bone while no repair was seen in the control animals without implants or when the defect was filled with FMBs alone.…”

Section: Different Forms Of Fibrin Applications In Bone Tissue Enginementioning

confidence: 99%

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A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

379

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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Section: Different Forms Of Fibrin Applications In Bone Tissue Enginementioning

confidence: 99%

Section: Different Forms Of Fibrin Applications In Bone Tissue Enginementioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

379

256

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“…30,32 It has been relatively rarely used for surface modification of scaffolds, with the exception of collagen-chitosan porous membranes, 31 or on electrospun poly(lactide-glycolide-caprolactone) nanofibers. 33 To the best of our knowledge, a combination of fibrin and ascorbic acid has not previously been used for constructing potential skin replacements, although this combination has been applied in tissue engineering of tendon and ligament, 34 cartilage, 35 bone, 36 smooth muscle, 37 and heart valves. 38 In these applications, ascorbic acid promoted appropriate differentiation and phenotypic maturation of cells and increased the collagen production in these cells.…”

mentioning

confidence: 99%

The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Bačáková¹,

Musı́lková²,

Riedel³

et al. 2016

IJN

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Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly( l -lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta 1 -integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering.

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“…[21][22][23] It is a natural, biodegradable fibrous protein commonly used for clinical and experimental tissue engineering applications, 24 including surgical tissue adhesives, 25 delivery of growth factors, 26 genes, 27 and cells 28 as well as tissue engineering for cartilage, 29,30 skin, 31 and bone. [32][33][34] The deposition of fibrin at early time-points…”

Section: Introductionmentioning

confidence: 99%

Physical and Biological Characterization of Ferromagnetic Fiber Networks: Effect of Fibrin Deposition on Short-Term In Vitro Responses of Human Osteoblasts

Spear

Srigengan

Neelakantan

et al. 2015

Tissue Engineering Part A

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Ferromagnetic fiber networks have the potential to deform in vivo imparting therapeutic levels of strain on ingrowing periprosthetic bone tissue. 444 Ferritic stainless steel provides a suitable material for this application due to its ability to support cultures of human osteoblasts (HObs) without eliciting undue inflammatory responses from monocytes in vitro. In the present article, a 444 fiber network, containing 17 vol% fibers, has been investigated. The network architecture was obtained by applying a skeletonization algorithm to three-dimensional tomographic reconstructions of the fiber networks. Elastic properties were measured using low-frequency vibration testing, providing globally averaged properties as opposed to mechanical methods that yield only local properties. The optimal region for transduction of strain to cells lies between the ferromagnetic fibers. However, cell attachment, at early time points, occurs primarily on fiber surfaces. Deposition of fibrin, a fibrous protein involved in acute inflammatory responses, can facilitate cell attachment within this optimal region at early time points. The current work compared physiological (3 and 5 g$L -1 ) and supraphysiological fibrinogen concentrations (10 g$L -1 ), using static in vitro seeding of HObs, to determine the effect of fibrin deposition on cell responses during the first week of cell culture. Early cell attachment within the interfiber spaces was observed in all fibrin-containing samples, supported by fibrin nanofibers. Fibrin deposition influenced the seeding, metabolic activity, and early stage differentiation of HObs cultured in the fibrin-containing fiber networks in a concentration-dependant manner. While initial cell attachment for networks with fibrin deposited from low physiological concentrations was similar to control samples without fibrin deposition, significantly higher HObs attached onto high physiological and supraphysiological concentrations. Despite higher cell numbers with supraphysiological concentrations, cell metabolic activities were similar for all fibrinogen concentrations. Further, cells cultured on supraphysiological concentrations exhibited lower cell differentiation as measured by alkaline phosphatase activity at early time points. Overall, the current study suggests that physiological fibrinogen concentrations would be more suitable than supraphysiological concentrations for supporting early cell activity in porous implant coatings.

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Fibrin Microbeads for Isolating and Growing Bone Marrow–Derived Progenitor Cells Capable of Forming Bone Tissue

Cited by 75 publications

References 19 publications

A review of fibrin and fibrin composites for bone tissue engineering

A review of fibrin and fibrin composites for bone tissue engineering

The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Physical and Biological Characterization of Ferromagnetic Fiber Networks: Effect of Fibrin Deposition on Short-Term In Vitro Responses of Human Osteoblasts

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