Fibrin network pattern changes of platelet-rich fibrin in young versus old age group of individuals: A cell block cytology study

Yajamanya, Shravanthi Raghav; Chatterjee, Anirban; Babu, Chaitanya N.; Karunanithi, Deepika

doi:10.4103/0972-124x.176390

Cited by 49 publications

(38 citation statements)

References 17 publications

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“…The relatively small sample size and the short-term follow-up may be not long enough to draw definitive conclusion on tooth survival. The age range of the included samples was quite broad (25–60 years), and this could have affected the present results, since some studies reported better outcomes for the use of PRF in young patients compared to elder ones [ 52 ]. A histomorphometric analysis would have been helpful to confirm the newly formed tissues but was not included in the selected studies, and indeed it is seldom feasible.…”

Section: Discussionmentioning

confidence: 99%

Use of Platelet-Rich Fibrin in the Treatment of Grade 2 Furcation Defects: Systematic Review and Meta-Analysis

Tarallo

Mancini

Pitzurra

et al. 2020

JCM

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In periodontitis patients, furcation defects are crucial sites to regenerate due to their complex anatomy. Various modern surgical techniques and use of biomaterials have been suggested in the literature. Among all, platelet-rich fibrin (PRF) has potential in tissue regeneration thanks to its role in the release of growth factors. Therefore, the purpose of this study was to evaluate the beneficial effect of the addition of PRF to open flap debridement (OFD) or as an adjuvant to other biomaterials such as bone grafts in the treatment of grade 2 mandibular furcation defects. Systematic research was carried out on the databases Medline, Scopus, Embase, and Cochrane Library and registered on PROSPERO (CRD42020167662). According to the PICO guidelines by Cochrane, randomized trials and prospective non-randomized trials were evaluated, with a minimum follow-up period of 6 months. The inclusion criteria were the absence of systemic diseases, non-smoking patients, and a population aged from 18 to 65 years. Vertical pocket probing depth (PPD), vertical clinical attachment level (VCAL), and gingival recession (REC) were the primary outcomes. Vertical furcation depth (VFD), and the percentage of bone defect fill (%v-BDF) were considered as secondary outcomes. A meta-analysis of the primary and secondary outcomes was performed. Publication bias was assessed through a funnel plot. Eighty-four articles were initially extracted. Eight randomized clinical trials were analyzed according to the exclusion and inclusion criteria. The Quality assessment instrument (QAI) revealed four articles at low risk of bias, one at moderate, and three at high risk of bias. The metanalysis showed significant data regarding PPD, VCAL, VFD and %v-BDF in the comparison between PRF + OFD vs. OFD alone. The adjunct of PRF to a bone graft showed a significant difference for VCAL and a not statistically significant result for the other involved parameters. In conclusion, the adjunctive use of PRF to OFD seems to enhance the periodontal regeneration in the treatment of grade 2 furcation defects. The combination of PRF and bone graft did not show better clinical results, except for VCAL, although the amount of literature with low risk of bias is scarce. Further well-designed studies to evaluate the combination of these two materials are therefore needed.

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Section: Discussionmentioning

confidence: 99%

Use of Platelet-Rich Fibrin in the Treatment of Grade 2 Furcation Defects: Systematic Review and Meta-Analysis

Tarallo

Mancini

Pitzurra

et al. 2020

JCM

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“…Therefore, we found that cytokine levels differed with age and sex. Of course, there could be genetic, hormonal, or environmental reasons for sex- and age-related differences in cytokine levels 25 – 28 .…”

Section: Discussionmentioning

confidence: 99%

Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels

Bai

Chuang

Lin

et al. 2018

Sci Rep

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We aimed to determine relationships between age and sex with cytokine content and distribution in human platelet-rich fibrin (PRF) gel. Rabbit PRF was harvested from whole blood (n = 6). Human PRF was collected from 36 healthy volunteers (1:1 men:women) without systemic diseases and not current undergoing medical treatment. Histological analysis and optical microscopy were used to assess the three-dimensional structure of the PRF network. Enzyme-linked immunosorbent assays, quantification of adenosine triphosphate, and bioluminescence imaging of PRF sections were used to assess cytokine and entrapped platelet distribution. Three-dimensional structures of fibrin networks revealed concentration gradients of the platelet-derived growth factor beta beta homodimer and the transforming growth factor-beta 1. Histological analysis of PRF sections (from the red blood cell end to the plasma end of a clot) showed a gradual increase in average porosity, most prominently in PRF clots from young and middle-aged men and women, and a decrease in compactness along the longitudinal axis of the PRF gel. The end of the PRF gel closest to the red blood cell layer is the essence of the PRF clot, and the ability to generate platelets depends on sex and age in humans.

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“…First, the centrifugation process, with characteristics as speed and g‐force, directly impacts the architecture of L‐PRF 20 . Second, the age of the patient of whom blood is taken for L‐PRF influences the fibrin network of L‐PRF 21 . These two factors must be kept in mind for in vivo applications of L‐PRF, but could not have been influencing factors in this in vitro study as all L‐PRF membranes were generated according to the same centrifugation protocol and were taken of volunteers with more or less the same age.…”

Section: Discussionmentioning

confidence: 99%

Mechanical and structural properties of leukocyte‐ and platelet‐rich fibrin membranes: An in vitro study on the impact of anticoagulant therapy

Ockerman

Braem

EzEldeen

et al. 2020

J of Periodontal Research

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Objective and background Little is known about structural and mechanical properties of leukocyte‐ and platelet‐rich fibrin (L‐PRF) membranes and even less about the influence of antithrombotic drugs on L‐PRF. The aim of this in vitro study is therefore to investigate mechanical properties, fibrin structure and cell content of L‐PRF membranes and the impact of anticoagulant therapy on L‐PRF. Methods Blood samples were obtained from 12 volunteers and supplemented with either no, 1.25 IU, 2.5 IU, 5 IU, or 10 IU enoxaparin. L‐PRF membranes were characterized with tensile testing, scanning electron microscopy, and measurement of platelets and leukocytes. Control and enoxaparin‐supplemented L‐PRF membranes were compared. Results At 10 IU enoxaparin, no L‐PRF membranes could be generated, whereas the low doses of 1.25 and 2.5 IU had no influence on L‐PRF properties. The mechanical properties, fibrin networks, and number of platelets and leukocytes of 5 IU supplemented membranes were unlike the control membranes, but were not found to be significantly different because of limited sampling and inter‐ and intra‐variability. Conclusion Low doses of the anticoagulant enoxaparin do not affect mechanical properties, fibrin network, nor cellular content of L‐PRF, whereas high doses impair L‐PRF generation.

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Fibrin network pattern changes of platelet-rich fibrin in young versus old age group of individuals: A cell block cytology study

Cited by 49 publications

References 17 publications

Use of Platelet-Rich Fibrin in the Treatment of Grade 2 Furcation Defects: Systematic Review and Meta-Analysis

Use of Platelet-Rich Fibrin in the Treatment of Grade 2 Furcation Defects: Systematic Review and Meta-Analysis

Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels

Mechanical and structural properties of leukocyte‐ and platelet‐rich fibrin membranes: An in vitro study on the impact of anticoagulant therapy

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