Fibrin Proliferation at Model Surfaces:  Influence of Surface Properties

Evans-Nguyen, Kenyon M.; Schoenfisch, Mark H.

doi:10.1021/la047303h

Cited by 52 publications

(60 citation statements)

References 29 publications

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“…Hence, φ should be considered as an important mechanical qualitative characteristic of normal fibrin polymer attached to the particular type of surface. One can expect that φ is sensitive to the to the type of coagulation test (KccT, PT, APTT) and to the choice of QCM coating material (see -Δf(t) & ΔГ(t) curves presented in some publications [3,24,34]). …”

Section: Resultsmentioning

confidence: 99%

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Modelling of blood component flexibility using quartz crystal microbalance

et al. 2014

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Quartz crystal microbalance (QCM) is widely used for the investigation of human blood mechanical properties, and shows high sensitivity for monitoring select dynamic processes, including red blood cell sedimentation and aggregation, coagulation, plasma protein absorption, as well as evaluating the bio-compatibility/affinity of different materials, etc. The present study provides a critical analysis of the challenges associated with data modelling and data interpretation, with respect to a QCM-based experimental setup. Here, the modelling approach selected for use is based on the analysis of the interactions that occur between an oscillating QCM surface and different blood components adhering to the surface. The resultant mathematical model overcomes these challenges by presenting simple, interpretable output parameters for monitoring experimental erythrocyte sedimentation in whole blood (WB), platelet aggregation in platelet rich plasma (PRP) and fibrin polymerisation in platelet poor plasma (PPP).

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Section: Resultsmentioning

confidence: 99%

“…It was demonstrated in [23,24,25] that plasma proteins (especially fibrinogen and albumin) are very absorbable to hydrophobic surfaces such as polystyrene, which is selected for use in the present study. Plasma proteins have small sizes (1-50 nm) relative to the shear wave penetration depth.…”

Section: Numerical Methods and Modellingmentioning

confidence: 99%

Modelling of blood component flexibility using quartz crystal microbalance

et al. 2014

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“…The deposition of SAMs has been studied extensively as a method to control interactions between solid substrates and biological systems, including the influence of surface chemistry on protein adsorption [105][106][107][108][109][110] and cell adhesion. [111][112][113][114][115] For example, SAMs of thiols bearing terminal carbohydrates ͓e.g., Fig.…”

Section: 104mentioning

confidence: 99%

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

et al. 2009

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This review focuses on the surface modification of substrates with self-assembled monolayers (SAMs) and polymer brushes to tailor interactions with biological systems and to thereby enhance their performance in bioapplications. Surface modification of biomedical implants promotes improved biocompatibility and enhanced implant integration with the host. While SAMs of alkanethiols on gold substrates successfully prevent nonspecific protein adsorption in vitro and can further be modified to tether ligands to control in vitro cell adhesion, extracellular matrix assembly, and cellular differentiation, this model system suffers from lack of stability in vivo. To overcome this limitation, highly tuned polymer brushes have been used as more robust coatings on a greater variety of biologically relevant substrates, including titanium, the current orthopedic clinical standard. In order to improve implant-bone integration, the authors modified titanium implants with a robust SAM on which surface-initiated atom transfer radical polymerization was performed, yielding oligo(ethylene glycol) methacrylate brushes. These brushes afforded the ability to tether bioactive ligands, which effectively promoted bone cell differentiation in vitro and supported significantly better in vivo functional implant integration.

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“…The IAsys setup enables continuous vibro-stirring in the cuvette [e.g., the stirrer vibrates vertically with a frequency of 126 Hz at amplitude selected by the user, maximum being 100 (equivalent to a displacement of 0.5 mm)] that ensures not only the rapid mixing thus minimizing mass-transport effect, but also retards the initiation of fibrin polymerization. According to the recent work of Evans-Nguyen and Schoenfisch, 40 in which the fibrin proliferation on adsorbed fibrinogen was monitored in real time using a quartz crystal microbalance (again with constant stirring), a steady level of surface-formed fibrin polymer was not observed during the first 10 min. Additionally, the accurate analysis (limited to the first 120 s of recorded data and to a final FM concentration < 500 nM) of association curves (see below, in IAsys Data Analysis section, for a detailed discussion) makes us belief that the association data, which were accumulated up to the first 120 s and for a FM concentration < 500 nM, correspond mostly to the binding of monomeric fibrin to immobilized fibrinogen.…”

Section: Measurement Of Desaabb-fmfibrinogen Interaction With Biosensorsmentioning

confidence: 99%

Kinetics of the interaction of desAABB–fibrin monomer with immobilized fibrinogen

et al. 2006

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The soluble and stable fibrin monomer-fibrinogen complex (SF) is well known to be present in the circulating blood of healthy individuals and of patients with thrombotic diseases. However, its physiological role is not yet fully understood. To deepen our knowledge about this complex, a method for the quantitative analysis of interaction between soluble fibrin monomers and surface-immobilized fibrinogen has been established by means of resonant mirror (IAsys) and surface plasmon resonance (BIAcore) biosensors. The protocols have been optimized and validated by choosing appropriate immobilization procedures with regeneration steps and suitable fibrin concentrations. The highly specific binding of fibrin monomers to immobilized fibrin(ogen), or vice versa, was characterized by an affinity constant of approximately 10(-8)M, which accords better with the direct dissociation of fibrin triads (KD approximately 10(-8) -10(-9) M) (J. R. Shainoff and B. N. Dardik, Annals of the New York Academy of Science, 1983, Vol. 27, pp. 254-268) than with earlier estimations of the KD for the fibrin-fibrinogen complex (KD approximately 10(-6) M) (J. L. Usero, C. Izquierdo, F. J. Burguillo, M. G. Roig, A. del Arco, and M. A. Herraez, International Journal of Biochemistry, 1981, Vol. 13, pp. 1191-1196).

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Fibrin Proliferation at Model Surfaces: Influence of Surface Properties

Cited by 52 publications

References 29 publications

Modelling of blood component flexibility using quartz crystal microbalance

Modelling of blood component flexibility using quartz crystal microbalance

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

Kinetics of the interaction of desAABB–fibrin monomer with immobilized fibrinogen

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