Fibrinogen and magnesium combination biomaterials modulate macrophage phenotype, NF-kB signaling and crosstalk with mesenchymal stem/stromal cells

Bessa-Gonçalves, Mafalda; Silva, Alexandre Rodrigues; Brás, João; Helmholz, Heike; Luthringer-Feyerabend, Bérengère; Willumeit‐Römer, Regine; Barbosa, Mário A.; Santos, Susana G.

doi:10.1016/j.actbio.2020.07.028

Cited by 50 publications

(26 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the scaffold material was implanted into the tissue, the phagocytosis of macrophages plays an important role in controlling inflammatory response ( Bessa-Gonçalves et al, 2020 ; Mahon et al, 2020 ; Yang et al, 2020 ). Macrophages can phagocytize apoptotic cells and eliminate pathogenic microorganisms, which is more conducive to the implantation of scaffold materials.…”

Section: Effects Of Hydrogel Stiffness On Tissue Repairmentioning

confidence: 99%

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

Luo

Tan

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Tissue repair after trauma and infection has always been a difficult problem in regenerative medicine. Hydrogels have become one of the most important scaffolds for tissue engineering due to their biocompatibility, biodegradability and water solubility. Especially, the stiffness of hydrogels is a key factor, which influence the morphology of mesenchymal stem cells (MSCs) and their differentiation. The researches on this point are meaningful to the field of tissue engineering. Herein, this review focus on the design of hydrogels with different stiffness and their effects on the behavior of MSCs. In addition, the effect of hydrogel stiffness on the phenotype of macrophages is introduced, and then the relationship between the phenotype changes of macrophages on inflammatory response and tissue repair is discussed. Finally, the future application of hydrogels with a certain stiffness in regenerative medicine and tissue engineering has been prospected.

show abstract

Section: Effects Of Hydrogel Stiffness On Tissue Repairmentioning

confidence: 99%

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

Luo

Tan

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Reducing the host responses through modulating macrophage polarization has been the focus of many recent studies. 18 , 21 – 24 In addition, the monocyte-macrophage cell lineage is known as a key player in bone regeneration and acute inflammatory response. This is largely owing to their high plasticity in response to environmental signals and their multiple roles in bone homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Early osteoimmunomodulatory effects of magnesium–calcium–zinc alloys

et al. 2021

View full text Add to dashboard Cite

Today, substantial attention is given to biomaterial strategies for bone regeneration, and among them, there is a growing interest in using immunomodulatory biomaterials. The ability of a biomaterial to induce neo vascularization and macrophage polarization is a major factor in defining its success. Magnesium (Mg)-based degradable alloys have attracted significant attention for bone regeneration owing to their biodegradability and potential for avoiding secondary removal surgeries. However, there is insufficient evidence in the literature regarding the early inflammatory responses to these alloys in vivo. In this study, we investigated the early body responses to Mg-0.45wt%Zn-0.45wt%Ca pin-shaped alloy (known as ZX00 alloy) in rat femora 2, 5, and 10 days after implantation. We used 3D micro computed tomography (µCT), histological, immunohistochemical, histomorphometrical, and small angle X-ray scattering (SAXS) analyses to study new bone formation, early macrophage polarization, neo vascularization, and bone quality at the implant bone interface. The expression of macrophage type 2 biological markers increased significantly after 10 days of Mg alloy implantation, indicating its potential in stimulating macrophage polarization. Our biomineralization results using µCT as well as histological stained sections did not indicate any statistically significant differences between different time points for both groups. The activity of alkaline phosphatase (ALP) and Runt-related transcription factor 2 (Runx 2) biological markers decreased significantly for Mg group, indicating less osteoblast activity. Generally, our results supported the potential of ZX00 alloy to enhance the expression of macrophage polarization in vivo; however, we could not observe any statistically significant changes regarding biomineralization.

show abstract

“…Our results demonstrated that both uExo and dExo had ROS-scavenging activities and significantly reduced the oxidative stress in rSCs. Several studies have reported that MSC-derived exosomes combat oxidative stress-induced damage through adaptive regulation of the nuclear factor erythroid 2-related factor 2 (Nrf2) defense system [ 59 , 90 , 91 ]. Both dExo and uExo significantly improved the ROS defense capacity compared to control group.…”

Section: Discussionmentioning

confidence: 99%

“…Monocytes isolated from peripheral blood mononuclear cells from anonymous human blood samples and were provided by the Elutriation Core Facility at the University of Nebraska Medical Center. Differentiation of monocytes into Mɸ and M1 macrophage was performed following a protocol modified from previous studies [ 59 , 60 ]. Briefly, 5 × 10 5 monocytes were seeded on sterile glass coverslips in 24-well plates and then cultured for 6 days in RPMI 1640 medium (Gibco) containing 10% FBS, 1% P/S, and 50 ng/mL of recombinant granulocyte macrophage colony-stimulating factor (GM-CSF, PeproTech).…”

Section: Methodsmentioning

confidence: 99%

Exosomes derived from differentiated human ADMSC with the Schwann cell phenotype modulate peripheral nerve-related cellular functions

Liu

Kong

Shi

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

Fibrinogen and magnesium combination biomaterials modulate macrophage phenotype, NF-kB signaling and crosstalk with mesenchymal stem/stromal cells

Cited by 50 publications

References 66 publications

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

Early osteoimmunomodulatory effects of magnesium–calcium–zinc alloys

Exosomes derived from differentiated human ADMSC with the Schwann cell phenotype modulate peripheral nerve-related cellular functions

Contact Info

Product

Resources

About