Fibrinogen determination according to Clauss: commutability assessment of International and commercial standards and quality control samples

Besselaar, A. M. H. P. Van Den; Rijn, Claudia J.J. van; Cobbaert, Christa; Reijnierse, G Louis A; Hollestelle, Martine J.; Niessen, R. W. L. M.; Hudig, Francisca

doi:10.1515/cclm-2016-1088

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…As elucidated in Table 1, different reagents and instruments produced by Stago, Siemens, and Instrumentation Laboratory are used to quantify fibrinogen concentration. It is known that there can be systematic differences between the fibrinogen concentrations obtained with various commercial kits [90]. The different detections used in the Clauss method and resuscitation fluids administered may affect the correlation [91, 92].…”

Section: Measurement Of Fibrinogen Levels and Functionsmentioning

confidence: 99%

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Peng

Nascimento

Beckett

2018

BioMed Research International

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Fibrinogen is crucial for the formation of blood clot and clinical outcomes in major bleeding. Both Thromboelastography (TEG) and Rotational Thromboelastometry (ROTEM) have been increasingly used to diagnose fibrinogen deficiency and guide fibrinogen transfusion in trauma and surgical bleeding patients. We conducted a comprehensive and comparative review on the technologies and clinical applications of two typical functional fibrinogen assays using TEG (FF TEG) and ROTEM (FIBTEM) for assessment of fibrinogen level and deficiency, and prediction of transfusion requirement. Clot strength and firmness of FF TEG and ROTEM FIBTEM were the most used parameters, and their associations with fibrinogen levels as measured by Clauss method ranged from 0 to 0.9 for FF TEG and 0.27 to 0.94 for FIBTEM. A comparison of the interchangeability and clinical performance of the functional fibrinogen assays using the two systems showed that the results were correlated, but are not interchangeable between the two systems. It appears that ROTEM FIBTEM showed better associations with the Clauss method and more clinical use for monitoring fibrinogen deficiency and predicting transfusion requirements including fibrinogen replacement than FF TEG. TEG and ROTEM functional fibrinogen tests play important roles in the diagnosis of fibrinogen-related coagulopathy and guidance of transfusion requirements. Despite the fact that high-quality evidence is still needed, the two systems are likely to remain popular for the hemostatic management of bleeding patients.

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Section: Measurement Of Fibrinogen Levels and Functionsmentioning

confidence: 99%

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Peng

Nascimento

Beckett

2018

BioMed Research International

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“…The pattern of results between different kits was similar for the two samples ( It is necessary to consider any matrix effect of such processing, and some authors have described a lack of commutability amongst different automated methods for some freeze-dried plasmas compared to fresh frozen samples. 9 There are further limitations and challenges to the provision of EQA material for interlaboratory comparability. Firstly, in some cases, very large numbers of laboratories carry out a particular investigation, and patient material is not available in sufficient volume to provide EQA.…”

Section: Direct Oral Anticoagulant (Doac) Assaysmentioning

confidence: 99%

The importance of commutability in material used for quality control purposes

Jennings¹,

Kitchen²,

Kitchen³

et al. 2018

Int J Lab Hematology

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External Quality Assessment (EQA) is an important part of laboratory quality assurance. Spiking of normal plasma is sometimes employed to mimic clinical samples. It is important that spiked material gives similar results to clinical samples (ie, is commutable) to ensure appropriate conclusions can be drawn from EQA exercises. We describe here data from UK National External Quality Assessment Scheme for Blood Coagulation (NEQAS BC) exercises where spiked samples were tested alongside samples from patients to explore commutability of artificial material. Normal plasma was spiked with unfractionated heparin (UFH), low molecular weight heparin (LMWH), Dabigatran or Rivaroxaban. Factor VIII (FVIII) deficient plasma was spiked with FVIII concentrate. Spiked samples and ex vivo patient material were sent to laboratories for testing. For LMWH, good agreement was seen between results for samples from patient plasma and plasma spiked with heparin. For UFH, APTT ratios differed between spiked and patient samples for the same drug concentration, with no correlation in ranking of reagents. Precision for patient and spiked material for Rivaroxaban and Dabigatran assays was comparable. However, the pattern of results for some Dabigatran assay kits differed between spiked and patient samples. For FVIII assays, results obtained with spiked and postinfusion samples gave comparable results. Spiked material is suitable for EQA if commutability is demonstrated. Our data show commutability for plasma spiked with Rivaroxaban, LWMH and some FVIII concentrates. For some tests, notably APTT for UFH, marked differences between patient and spiked samples indicate not all tests can be evaluated using artificial samples.

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“…For the preparation of the PA hydrogels, a solution (1 mL) of plateletpoor human plasma (with a fibrinogen concentration of 2.5 mg/mL assessed by the Clauss fibrinogen assay This assay shows and SD of 0.7 mg/mL as reported in the literature [20,21]), 140 μL 150 mM NaCl, 14 μL of tranexamic acid (5 mg/mL) (CAS Number 1197-18-8, Sigma), and 40 μL of a fibroblast suspension (2.5 × 10 [5] cells/mL) was mixed with 0 %, 0.5 %, 1 %, 1.5 % or 2 % (w/v) of agarose type VII (CAS 9012366, Sigma, USA). The final concentrations of agarose in the PA hydrogels were adjusted by conveniently varying the plasma volumes (67 %-27 %).…”

Section: Hydrogel Preparationmentioning

confidence: 90%

Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications

Vargas

Martínez-García

Offens

et al. 2022

Biomaterials Advances

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Fibrinogen determination according to Clauss: commutability assessment of International and commercial standards and quality control samples

Cited by 12 publications

References 15 publications

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

The importance of commutability in material used for quality control purposes

Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications

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