Fibrinolytic activity of kaempferol isolated from the fruits ofLagenaria siceraria(Molina) Standley

Abstract

This study was undertaken to isolate a flavonol, kaempferol, from the fruits of Lagenaria siceraria (bottle gourd) as a sole compound and to explore the fibrinolytic potential of the methanolic extract of the fruits of L. siceraria and the isolated compound using their in vitro activity. The fibrinolytic activity in terms of percentage of plasma clot liquefaction was determined by plasma clot lysis at 37°C in 24 h. The fibrinolytic activity of both substances was compared to the well-known thrombolytic agent streptokinase (30,000 IU). The percentage of fibrinolytic activity of the extract and isolated compound were found to be 54.72 ± 0.7210 and 77.37 ± 1.3010, respectively. Streptokinase was considered as the standard fibrinolytic enzyme for comparative purposes and had 91.46 ± 0.7625% fibrinolytic activity. The conclusion drawn in our study after testing the hypothesis by experimental procedures is that in vitro fibrinolytic activity on plasma clots is an inherent property of kaempferol isolated from the fruits of L. siceraria, and its comparison with streptokinase is a new aspect for further study.

Phytochemical Studies

“…Apigenin also inhibits glucosyltransferases and phosphodiesterase activity (Rajnarayana et al 2001;De Sanchez et al 1996;Koo et al 2002). Kaempferol has shown in vitro fibrinolytic activity (Rajput et al 2011) and exerts a potent inhibitory effect on in vitro bone resorption (Lorget et al 2003) along with estrogenic activity (Zoechling et al 2009). It is a potent chemopreventive agent against skin cancer through its inhibitory interaction with Src (Lee et al 2010).…”

Role of Pakshaghata Chikitsasutra in the Management of Ischemic Heart Disease (IHD) w.s.r to Herbal Antithrombotic Drugs

“…International Journal of Advanced Ayurveda, Yoga, Unani, Siddha and Homeopathy 343 fibrinolytic activity comparable to streptokinase activity [47]. An ethanolic extract of L. siceraria fruit had a membrane-stabilising role in isoproterenol-induced myocardial infarction in rats [48].…”

Anticoagulant and Fibrinolytic Activities of Hwanggeumchal Sorghum In Vitro

“…In addition, a plant phenolic compound, kaempferol has been shown to possess the fibrinolytic activity [32]. Although characteristics of the anticoagulant ingredients of hwanggeumchal sorghum grains remain to be elucidated, it seems likely that the 80% ethanol extract of hwanggeumchal sorghum grains might have potential antithrombotic effects in that it could exert fibrinolytic as well as anticoagulant activities.…”