Fibrinolytic protease from<i>Bacillus cereus</i>S46: Purification, characterization, and evaluation of its in vitro thrombolytic potential

D'Souza, Desrie H.; Bhattacharya, Sourav; Das, Arijit

doi:10.1002/jobm.202000148

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…The enzyme was purified 1714-fold relative to the original crude enzyme solution, and the specific activity of the final enzyme product was 120 U/mg protein. Ion-exchange chromatography for enzyme purification is also frequently reported [144][145][146].…”

Section: Purificationmentioning

confidence: 99%

Enzyme Recovery from Biological Wastewater Treatment

Liu¹,

Smith²

2020

Waste Biomass Valor

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Enzymes are high value industrial bio-catalysts with extensive applications in a wide range of manufacturing and processing sectors, including the agricultural, food and household care industries. The catalytic efficiency of enzymes can be several orders higher compared to inorganic chemical catalysts under mild conditions. However, the nutrient medium necessary for biomass culture represents a significant cost to industrial enzyme production. Activated sludge (AS) is a waste product of biological wastewater treatment and consists of microbial biomass that degrades organic matter by producing substantial quantities of hydrolytic enzymes. Therefore, enzyme recovery from AS offers an alternative, potentially viable approach to industrial enzyme production. Enzyme extraction from disrupted AS flocs is technically feasible and has been demonstrated at experimental-scale. A critical review of disruption techniques identified sonication as potentially the most effective and suitable method for enzyme extraction, which can be scaled up and is a familiar technology to the water industry. The yields of different enzymes are influenced by wastewater treatment conditions, and particularly the composition, and can also be controlled by feeding sludge with specific target substrates. Nevertheless, hydrolytic enzymes can be effectively extracted directly from waste AS without specific modifications to standard wastewater treatment processes. Purification, concentration and stabilisation/immobilisation techniques can also greatly expand the industrial application and increase the economic value and marketability of enzyme products extracted from AS. Concentrated and purified AS enzymes could readily substitute inorganic and/or commercial bioenzyme catalysts in many industrial applications including, for example, leather processing, and in detergent and animal feed formulation. Enzyme extraction from AS therefore offers significant economic benefits to the Water Industry by recovering valuable resources from wastewater. They can also optimise important waste treatment processes, such as the anaerobic digestion (AD) of sewage sludge, increasing biogas and renewable energy production. The enzyme-extracted sludge exhibits improved treatment properties, such as increased settleability, dewaterability, and anaerobic digestibility for biogas production, assisting sludge management by wastewater treatment plants (WWTPs) and enabling the further utilisation of the residual sludge. Graphic Abstract

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Section: Purificationmentioning

confidence: 99%

Enzyme Recovery from Biological Wastewater Treatment

Liu¹,

Smith²

2020

Waste Biomass Valor

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“…Over the years, many foods derived from fibrinolytic enzymes have been found in a variety of traditional Asian foods (Peng et al, 2003 ; Weng et al, 2017 ; Yang et al, 2020 ). During the fermentation process, microorganisms produce various enzymes that have been isolated, purified, and characterized by salting out of proteins using ammonium sulfate precipitation, ion‐exchange chromatography, gel‐filtration (GF) chromatography, and assay of fibrinolytic activity (D'Souza et al, 2020 ; Hu et al, 2019 ; Kim et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Research progress on the fibrinolytic enzymes produced from traditional fermented foods

Wang

Peng

Xie

et al. 2023

Food Science & Nutrition

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Cardiovascular diseases (CVDs) are a global health problem and leading cause of death worldwide. Thrombus formation, one of the CVDs, is essentially the formation of fibrin clots. The existing thrombolytic agents have the disadvantages of high price, short half‐life, and high bleeding risk; hence, there is an urgent need to find the alternative thrombolytic agents. In recent years, traditional fermented foods have been widely investigated for their outstanding effects in the prevention and treatment of thrombus formation. In this review, we have focused on fibrinolytic enzymes produced by microorganisms during the fermentation of traditional fermented foods and their potential use for treating CVDs. First, we discussed about the sources of fibrinolytic enzymes and microbial strains that produce those enzymes followed by the optimization of fermentation process, purification, and physicochemical properties of fibrinolytic enzymes. Finally, we have summarized the thrombolytic effects of fibrinolytic enzymes in humans and mice. Fibrinolytic enzymes produced by microorganisms during the fermentation of traditional fermented foods not only lyse thrombi but also acts as anti‐atherosclerotic, anti‐hyperlipidemia, and neuroprotection agents. Therefore, fibrinolytic enzymes from traditional fermented foods have great potential for the prevention and treatment of CVDs.

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