Fibroblast and Epidermal Growth Factors Modulate Proliferation and Neural Cell Adhesion Molecule Expression in Epithelial Cells Derived From the Adult Mouse Tongue

Ookura, Tetsuya; Kawamoto, Keiko; Tsuzaki, Hidetoshi; Mikami, Yuzuru; Ito, Yumiko; Oh, Seong-Hee; Hino, Akihiro

doi:10.1290/1071-2690(2002)038<0365:faegfm>2.0.co;2

Cited by 17 publications

(21 citation statements)

References 31 publications

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“…Cell culture KT-1 cells were cultured and passaged as previously described with some modifications (Ookura et al 2002). Chelated fetal bovine serum was replaced with a cell-culture-grade bovine serum albumin (Proliant Biologicals, Ankeny, IA).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we isolated and cultured integrinb 1 -positive cells from mouse-tongue epithelium, and established cell line KT-1 (Ookura et al 2002). We found that EGF and FGF-2 stimulated proliferation of KT-1 cells and that calcium ion (1 mM) promoted multilayered cell differentiation, suggesting that this cell line is useful for analyzing the growth and differentiation of tongue epithelial cells.…”

Section: Introductionmentioning

confidence: 94%

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Tongue epithelial KT-1 cell-cycle arrest by TGF-β associated with induction of p21Cip1 and p15Ink4b

2009

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Tongue epithelium continuously turns over in adults. Our previous study showed that epidermal growth factor and fibroblast growth factor-2 stimulated proliferation of KT-1 cells derived from tongue epithelium, suggesting that these signals serve as positive regulators for tongue epithelial proliferation. To investigate a negative regulation of tongue epithelial cell proliferation, we studied effects of transforming growth factor-beta (TGF-beta) on KT-1 cells. Proliferation assays showed that TGF-beta inhibited proliferation of KT-1 cells in a dose dependent manner. Cell-cycle analysis showed that TGF-beta induced G(0)/G(1) cell cycle arrest in KT-1 cells. We also examined expressions of Ink4 and Cip/Kip family mRNA by quantitative reverse transcription-polymerase chain reaction. We found that TGF-beta induced p15(Ink4b) and p21(Cip1) mRNA expressions. These results strongly suggest that G(0)/G(1) cell cycle arrest is associated with increased p15(Ink4b) and p21(Cip1) expressions. Moreover, p21(Cip1) mRNA was localized in suprabasal cells of tongue epithelium, suggesting that p21(Cip1) play a role in cell-cycle exit along with tongue epithelial differentiation. Taken together, our results suggest that TGF-beta signaling serves as negative regulator of tongue epithelial cell proliferation, and may control tongue epithelial cell differentiation through modulating expression of p21(Cip1).

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Tongue epithelial KT-1 cell-cycle arrest by TGF-β associated with induction of p21Cip1 and p15Ink4b

2009

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“…The present study firstly reported an establishment of clonal cell lines from mouse taste buds. A lingual epithelial cell line (KT-1) was established from an adult mouse tongue (Ookura et al 2002) and used for analysis of regulation in growth and differentiation (Nakamura et al 2009). The line expressed NCAM, suggesting possible linage-linkage to taste cells.…”

Section: Pkd1l3mentioning

confidence: 99%

“…Cell lines are used to investigate growth and differentiation of lingual gustatory epithelial cells and taste transduction systems. Correlation between NCAM expression and FGF-2 stimulation in tongue epithelial cells was investigated using KT-1 cells established from mouse gustatory papillae (Ookura et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Establishment of clonal cell lines of taste buds from a p53 −/− mouse tongue

Sako

Hori

Masuho

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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A taste bud is a sensory organ and consists of 50-100 spindle-shaped cells. The cells function as taste acceptors. They have characteristics of both epithelial and neuronal cells. A taste bud contains four types of cells, type I, type II, type III cells, and basal cells. Taste buds were isolated from a tongue of a p53-deficient mouse at day 12, and 11 clonal taste bud (TBD) cell lines were established. In immunochemical analysis, all cell lines expressed cytokeratin 18, gustducin, T1R3, and neural cellular adhesion molecule, but not GLAST. In RT-PCR analysis, shh was not expressed in any of the cell lines. Further analysis with RT-PCR was conducted on four cell lines. They expressed G protein-coupled taste receptors; T1R3, T2R8 for sweet, bitter, umami. And they also expressed α-ENaC for salty taste. While, a candidate for sour receptor HCN4 was expressed in TBD-a1 and TBD-a7 lines. And another candidate for sour receptor PKD1L3 was slightly expressed in TBD-a1 and TBD-c1.

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“…This is likely due to the small size of the oral cavity in mice and the limited amount of tissue available. However, one study has reported the development of a lingual epithelial cell line of mouse origin (KT-1 cells) (19). The KT-1 cells express markers consistent with a differentiation into gustatory epithelium rather than conventional oral epithelium.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin

Parikh

Nagarajan

Matsui

et al. 2008

Archives of Oral Biology

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Objective-To establish an oral epithelial cell line of mouse origin for molecular and biochemical assays.Design-Epithelial cells were isolated from the oral cavity of adult mice and established as a spontaneously immortalized cell line in culture, designated immortalized oral keratinocyte cells (IMOK cells). The cells were then characterized for growth characteristics, differentiation potential, karyotype, transfectability, susceptibility to viral infection and responses to siRNA.Results-The IMOK cells exhibit robust growth in both serum-containing and serum-free medium for at least 100 population doublings. IMOK cells have a near diploid karyotype, express keratinocyte marker proteins and can be induced to undergo differentiation by addition of high levels of calcium to the medium. The differentiation process is characterized by morphological changes and by the induction of oral epithelium specific differentiation marker sproteins such as K4 and K13. Transient transfection analyses reveal that IMOK cells are highly transfectable and that several promoters of epithelial cells are active in these cells. Moreover, upon differentiation with calcium, there is an upregulation of differentiation-specific K4 and Elf5 promoter activity. Finally, we show that the oral keratinocytes are also amenable to infection with retroviruses and to siRNA based knock-down of gene expression.Conclusions-Our study is the first to establish an immortalized oral keratinocyte cell line of murine origin that can recapitulate the oral epithelium differentiation program and thus could serve as a useful tool for toxicological and molecular analyses of the oral tissue.

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Fibroblast and Epidermal Growth Factors Modulate Proliferation and Neural Cell Adhesion Molecule Expression in Epithelial Cells Derived From the Adult Mouse Tongue

Cited by 17 publications

References 31 publications

Tongue epithelial KT-1 cell-cycle arrest by TGF-β associated with induction of p21Cip1 and p15Ink4b

Tongue epithelial KT-1 cell-cycle arrest by TGF-β associated with induction of p21Cip1 and p15Ink4b

Establishment of clonal cell lines of taste buds from a p53 −/− mouse tongue

Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin

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