Fibroblast culture on surface-modified poly (glycolide-co-ε-caprolactone) scaffold for soft tissue regeneration

Abstract

Novel porous matrices made of a copolymer of glycolide (G) and epsilon-caprolactone (CL) (51 : 49, Mw 103000) was prepared for tissue engineering using a solvent-casting particulate leaching method. Poly(glycolide-co-epsilon-caprolactone) (PGCL) copolymer showed a rubber-like elastic characteristic, in addition to an amorphous property and fast biodegradability. In order to investigate the effect on the fibroblast culture, PGCL scaffolds of varying porosity and pore size, in addition to surface-hydrolysis or collagen coating, were studied. The large pore-sized scaffold (pore size >150 microm) demonstrated a much greater cell adhesion and proliferation than the small pore-sized one. In addition, the higher porosity, the better the cell adhesion and proliferation. The surface-hydrolyzed PGCL scaffold showed enhanced cell adhesion and proliferation compared with the unmodified one. Type I collagen coating revealed a more pronounced contribution for increased cell interactions than the surface-hydrolyzed one. These results demonstrate that surface-modified PGCL scaffold can provide a suitable substrate for fibroblast culture, especially in the case of soft tissue regenerations.

Polymeric scaffolds in tissue engineering: a literature review

“…Kwon et al also studied fibroblast attachment and proliferation on PGCL scaffolds with different pore size range (22–300 μm). They demonstrated that scaffolds with pore size in the range of 150–300 μm supported more adhesion and proliferation of cells . Interestingly, Conde et al did not observed any difference in proliferation as well as odontogenic differentiation of DPSCs on PLLA scaffold with pore size range from 150 to 450 μm .…”

“…They showed improved hepatic cell adhesion and proliferation as well as albumin secretion . Four studies were also shown that coating of scaffold surface with various materials has impact on cell attachment and proliferation . In studies by Mashhadikhan et al, Koh et al, and Stendahl et al, surface of PLLA scaffolds were coated using gelatin, laminin, and cholesterol segments, respectively.…”

“…Pore architecture, including pore size, porosity, pore wall architecture, and pore interconnectivity, is the feature that is highly controllable in polymers. From reviewed papers, 10 articles evaluated following features on behavior of various cell types . Two Studies by Pamula group have shown high degree of osteoblast infiltration and proliferation in PLGA scaffolds with pore size of 400–600 μm and porosity of around 83% and 88% .…”

“…From reviewed papers, 23 articles have been evaluated the effect of chemical modifications on cell behaviors . It has been shown that surface topographic features of scaffolds can be modified with numerous approaches .…”

Functionalized Nanomaterials

“…The co-use of these adhesion proteins and biodegradable synthetic polymers enables the construction of cell-adhesive scaffolds for vitally functioning engineered tissues (Almany and Seliktar 2005;Chen et al 2000;Kwon et al 2001;Zhang et al 2005a). Co-electrospinning is a feasible approach to provide a compromise solution for overcoming the shortcomings of synthetic and natural polymers that is producing new porous nanofibrous biomaterials with good biocompatibility and improved mechanical, physical and chemical properties and biological performance.…”