Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro

Dzobo, Kevin; Turnley, Taegyn; Wishart, Andrew; Rowe, Arielle; Kallmeyer, Karlien; Vollenstee, Fiona A. van; Thomford, Nicholas Ekow; Dandara, Collet; Chopera, Denis; Pepper, Michael Sean; Parker, M. Iqbal

doi:10.3390/ijms17081259

Cited by 55 publications

(91 citation statements)

References 60 publications

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“…The scaffolds showed an ability to provide a suitable environment for human adipose-derived stem cells' survival and chondrogenesis. Another finding also supported by the studies that showed cartilage tissue formation when the AdSCs mixed with fibrin glue [25], as well as seeded in PRP-derived scaffold [23] or fibroblastderived extracellular matrix [26]. This result confirms the scaffold resulted in this study has good biocompatibility to the AdSCs and were suitable to be used in cartilage tissue engineering.…”

Section: ) Contact Angle and Water Uptakessupporting

confidence: 83%

Development of Salt Leached Silk Fibroin Scaffold using Direct Dissolution Techniques for Cartilage Tissue Engineering

Wibowo¹,

Judawisastra²,

Barlian³

et al. 2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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Autologous transplantations, the gold standard, did not meet sufficient health tissue coverage area for cartilage damage treatments. The field of tissue engineering offers a promising alternative to fulfill this limitation by growing patient own cells on biomaterials through tissue culture, reconstructed into new cartilage tissue, and the implanted to the injury area. To support tissue regeneration, biocompatible, biodegradable, and high strength silk fibroin (SF) was proposed in this study as scaffold materials. In this research, direct dissolution in CaCl 2 /formic acid, a faster and simpler process than traditional dissolution techniques, combined with salt leaching technique. SF contents on the scaffold were varied from 2 w/v% to 12 w/v% and NaCl size as porogen was fixed in diameter of 250±58 µm. Evaluation of the SF scaffold's morphology, hydrophilicity, biodegradability, and biocompatibility were conducted. The results showed porous silk fibroin scaffold had been successfully developed. The SF scaffolds have pore size 261-293 µm with highly interconnected pores. FTIR and XRD analysis of the scaffolds showed the characteristics of silk fibroin, which reveals the α-helix amorphous and β-sheet crystalline structure and comparable to the silk fibers. The scaffold showed good hydrophilicity and high water uptakes, which essential properties for cell survival. The scaffold degraded under Protease XIV, indicate biodegradable properties. Observation of cell attachment confirms the scaffold has good biocompatibility to adipose-derived stem cells and are suitable to be used in cartilage tissue engineering.

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Section: ) Contact Angle and Water Uptakessupporting

confidence: 83%

Development of Salt Leached Silk Fibroin Scaffold using Direct Dissolution Techniques for Cartilage Tissue Engineering

Wibowo¹,

Judawisastra²,

Barlian³

et al. 2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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“…The human dECM bioink induced high cell viability over a period of time and also induced the expression of several adipogenic proteins with no supplementation of adipogenic factors [86]. Overall, human adipose tissue can be a source of dECM bioinks and also stem cells [1,86,90,91]. Rat and goat tissue and organs such as adipose tissue, heart and liver are additional sources of dECM bioinks [92][93][94].…”

Section: Decellularized Ecm As Bioinkmentioning

confidence: 99%

“…Tissue-and organ-derived dECMs also present problems when it comes to large scale in vitro analysis, something that is possible with cell-derived dECMs. Thus, in some instances, cell derived ECMs may be better than tissue or organ derived ECMs [1,91,123]. When cells are cultured, they synthesize, secrete and assemble ECM components around them.…”

Section: Cell-derived Ecms Versus Tissue/organ-derived Ecmsmentioning

confidence: 99%

“…Such mesenchymal stem cell-derived ECMs are able to maintain stem cells in their undifferentiated state in vitro [17]. Several studies have shown that fibroblasts can produce ECMs rich in collagens type I, II, III and fibronectin [91,116,122,124]. With so many advantages over tissue or organ derived ECMs, cell derived ECMs are an appealing source of dECM bioinks.…”

Section: Cell-derived Ecms Versus Tissue/organ-derived Ecmsmentioning

confidence: 99%

“…Both cell-derived and tissue/organ-derived ECMs have been used is several studies and have shown the capability to direct cellular behavior ( Table 2). Cartilage Extracellular matrix Chondrogenic differentiation [133,134] Bladder Extracellular matrix Promotion of cellular proliferation and stemness [135,136] A fibroblast-derived extracellular matrix (fd-ECM) was able to induce chondrogenic differentiation of adipose-derived mesenchymal stromal cells (ad-MSCs) in vitro [91]. Although the Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 21 August 2019 doi:10.20944/preprints201908.0222.v1…”

Section: Cell-derived Ecms Versus Tissue/organ-derived Ecmsmentioning

confidence: 99%

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Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

Dzobo¹,

Motaung²,

Adesida³

2019

Preprint

Self Cite

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The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, all damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix, cells and inductive biomolecules. Currently, regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. Tissues and organs have a specific ECM, with specific proteins and factors released by cells residing within the local microenvironment. The coupling of regenerative medicine and tissue engineering field with 3D printing is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

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Periosteal Tissue Engineering: Current Developments and Perspectives

Lou

Wang

et al. 2021

Adv Healthcare Materials

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Periosteum, a highly vascularized bilayer connective tissue membrane plays an indispensable role in the repair and regeneration of bone defects. It is involved in blood supply and delivery of progenitor cells and bioactive molecules in the defect area. However, sources of natural periosteum are limited, therefore, there is a need to develop tissue‐engineered periosteum (TEP) mimicking the composition, structure, and function of natural periosteum. This review explores TEP construction strategies from the following perspectives: i) different materials for constructing TEP scaffolds; ii) mechanical properties and surface topography in TEP; iii) cell‐based strategies for TEP construction; and iv) TEP combined with growth factors. In addition, current challenges and future perspectives for development of TEP are discussed.

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Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro

Cited by 55 publications

References 60 publications

Development of Salt Leached Silk Fibroin Scaffold using Direct Dissolution Techniques for Cartilage Tissue Engineering

Development of Salt Leached Silk Fibroin Scaffold using Direct Dissolution Techniques for Cartilage Tissue Engineering

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

Periosteal Tissue Engineering: Current Developments and Perspectives

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