Fibroblast Growth Factor-2 Is Expressed by the Bovine Uterus and Stimulates Interferon-τ Production in Bovine Trophectoderm

Michael, Donna D.; Alvarez, Idania M.; Ocón, Olga; Powell, Anne M.; Talbot, Neil C.; Johnson, Sally; Ealy, Alan D.

doi:10.1210/en.2006-0234

Cited by 100 publications

(97 citation statements)

References 55 publications

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“…This laboratory previously described a role for FGF2 in stimulating IFNT production in bovine conceptuses (Michael et al 2006, Rodina et al 2008. Initially, the uterine epithelium was proposed as the primary site of FGF2 production in cattle (Michael et al 2006), but work contained herein and other findings (Daniels et al 2000, Lazzari et al 2002, Ocon-Grove et al 2008 establish that bovine conceptuses also produce FGF2.…”

Section: Discussionmentioning

confidence: 94%

“…FGF7 was not amplified in day 17 conceptus and CT1 samples but was detected in control samples (endometrium and lung). Bovine endometrium was included as a positive control based on previous work describing endometrial FGF1, 2, 7, and 10 expression in cyclic and pregnant cows and ewes (Chen et al 2000, Pfarrer et al 2006, Michael et al 2006, Ocon-Grove et al 2008. DNA sequencing verified that each PCR product represented the FGFs of interest (data not shown).…”

Section: Fgfr Subtype Expression In Bovine Conceptusesmentioning

confidence: 93%

“…Each FGFR was also detected in CT1 cells (Fig. 1), a bovine trophectoderm cell line derived from a bovine blastocyst that produces IFNT (Talbot et al 2000, Michael et al 2006. Amplified products of the correct size were detected in all samples with the exception of one day 17 conceptus sample that consistently lacked an FGFR1 amplicon (Fig.…”

Section: Fgfr Subtype Expression In Bovine Conceptusesmentioning

confidence: 93%

“…One of these is fibroblast growth factor 2 (FGF2). FGF2 is expressed by the luminal and glandular epithelium and detected in the uterine lumen of cows and ewes throughout the estrous cycle and early pregnancy (Michael et al 2006, Ocon-Grove et al 2008. Studies using a bovine trophectoderm cell line (CT1) and in vitro-produced (IVP) bovine blastocysts reveal that supplementation with FGF2 increases IFNT mRNA and protein levels (Michael et al 2006, Rodina et al 2008.…”

Section: Introductionmentioning

confidence: 99%

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Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Cooke¹,

Pennington²,

Yang³

et al. 2009

Reproduction

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The trophectoderm-derived factor interferon tau (IFNT) maintains the uterus in a pregnancy-receptive state in cattle and sheep. Fibroblast growth factors (FGFs) are implicated in regulating IFNT expression and potentially other critical events associated with early conceptus development in cattle. The overall objectives of this work were to identify the various FGFs and FGF receptors (FGFRs) expressed in elongating pre-attachment bovine conceptuses and determine if these FGFs regulate conceptus development and/or mediate IFNT production. In vitro-derived bovine blastocysts and in vivo-derived elongated conceptuses collected at day 17 of pregnancy express at least four FGFR subtypes (R1c, R2b, R3c, R4). In addition, transcripts for FGF1, 2, and 10 but not FGF7 are present in elongated bovine conceptuses. The expression pattern of FGF10 most closely resembled that of IFNT, with both transcripts remaining low in day 8 and day 11 conceptuses and increasing substantially in day 14 and day 17 conceptuses. Supplementation with recombinant FGF1, 2 or 10 increased IFNT mRNA levels in bovine trophectoderm cells and bovine blastocysts and increased IFNT protein concentrations in trophectoderm-conditioned medium. Blastocyst development was not affected by any of the FGFs. In summary, at least four FGFRs reside in pre-and peri-attachment bovine conceptuses. Moreover, conceptuses express at least three candidate FGFs during elongation, the time of peak IFNT expression. These findings provide new insight for how conceptus-derived factors such as FGF1, 2, and 10 may control IFNT expression during early pregnancy in cattle.

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Section: Discussionmentioning

confidence: 94%

Section: Fgfr Subtype Expression In Bovine Conceptusesmentioning

confidence: 93%

Section: Fgfr Subtype Expression In Bovine Conceptusesmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Cooke¹,

Pennington²,

Yang³

et al. 2009

Reproduction

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show abstract

“…The quantity of biologically active IFNT in conditioned medium was determined using a cytopathic antiviral assay (Michael et al 2006;Roberts et al 1989). Samples were examined in duplicate by titrating in a 96-well plate (1 : 3 serial dilution).…”

Section: Analysis Of Ifntmentioning

confidence: 99%

Paradoxical effect of supplementary progesterone between Day 3 and Day 7 on corpus luteum function and conceptus development in cattle

O'Hara¹,

Forde²,

Carter³

et al. 2014

Reprod. Fertil. Dev.

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Abstract. The aim of the present study was to investigate the effect of short-term progesterone (P4) supplementation during the early metoestrous period on circulating P4 concentrations and conceptus development in cattle. The oestrous cycles of cross-bred beef heifers were synchronised using a 7-day P4-releasing intravaginal device (PRIDÒ Delta; 1.55 g P4) treatment with administration of a prostaglandin F 2a analogue (Enzaprost; CEVA Sante Animale) the day before PRIDÒ Delta removal. Only those heifers recorded in standing oestrus (Day 0) were used. In Experiment 1, heifers were randomly assigned to one of five groups: (1) control: no treatment; (2) placebo: insertion of a blank device (no P4) from Day 3 to Day 7; (3) insertion of a PRIDÒ Delta from Day 3 to Day 7; (4) insertion of a PRIDÒ Delta from Day 3 to Day 5; or (5) insertion of a PRIDÒ Delta from Day 5 to Day 7. In vitro-produced blastocysts were transferred to each heifer in Groups 2-5 on Day 7 (n ¼ 10 blastocysts per heifer) and conceptuses were recovered when heifers were killed on Day 14. Based on the outcome of Experiment 1, in Experiment 2 heifers were artificially inseminated at oestrus and randomly assigned to one of three treatment groups: (1) placebo; (2) PRID from Day 3 to Day 5; or (3) PRID from Day 3 to Day 7. All heifers were killed on Day 16 and recovered conceptuses were incubated in synthetic oviducal fluid medium for 24 h; spent media and uterine flushes were analysed for interferon-t (IFNT). In both experiments, daily blood samples were taken to determined serum P4 concentrations. Data were analysed using the PROC MIXED procedure of SAS (SAS Institute, Cary, NC, USA). Insertion of a PRID resulted in an increase (P , 0.05) in serum P4 that declined following removal. In Experiment 1, P4 supplementation from Day 3 to Day 5 (17.0 AE 1.4 mm) or Day 3 to Day 7 (11.3 AE 2.3 mm) increased conceptus length compared with placebo (2.1 AE 1.8 mm). Serum P4 was significantly lower from Day 9 to Day 14 (P , 0.05) and the weight of the Day 14 corpus luteum (CL) was lower in the PRID Day 3-7 group than the placebo or control groups. In Experiment 2, supplementation from Day 3 to Day 5 (94.0 AE 18.8 mm) or Day 3 to Day 7 (143.6 AE 20.6 mm) increased conceptus length on Day 16 compared with placebo (50.3 AE 17.4 mm). Serum P4 was significantly lower in the two supplemented groups following PRID removal compared with placebo (P , 0.05) and was associated with a lower CL weight in the Day 3-7 group. Conceptus length was strongly correlated with the IFNT concentration in the uterine flush (r ¼ 0.58; P ¼ 0.011) and spent culture medium (r ¼ 0.68; P , 0.002). The findings of the present study highlight the somewhat paradoxical effects of P4 supplementation when given in the early metoestrous period in terms of its positive effect on conceptus development and its potentially negative effects on CL lifespan.

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Region‐Specific Expression of Aquaporin Subtypes in Equine Testis, Epididymis, and Ductus Deferens

Klein

Troedsson²,

Rutllant

2013

The Anatomical Record

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The process of water movement in the excurrent duct system of the male reproductive tract is pivotal for establishment of male fertility. The objective was to elucidate expression of aquaporin (AQP) water channels in the stallion reproductive tract. Real-time RT-PCR detected expression of AQP0-5 and AQP7-11 in testis, epididymis, and ductus deferens of mature stallions. There were two main expression patterns: (1) higher expression in testis than in epididymis and ductus deferens (AQP0, 24, 25, 28, 210, and 211); and (2) lower expression in testis than in epididymis and ductus deferens (AQP1, 23, 27, and 29). Overall, we inferred that fluid transport in the stallion testicle involved a collaboration of AQP subtypes (primarily AQP2, 25, 27, and 28). Based on immunohistochemistry, expression of AQP subtypes analyzed (i.e., AQP0, 22, 25, and 29) was localized to Leydig cells and elongated and round spermatids. Functional significance of AQP expression by Leydig cells remained uncertain. In elongated and round spermatids, AQP s likely contributed to the volume reduction observed during spermatogenesis. Subtypes AQP2 and AQP9 were the predominant forms expressed in epididymal tissue. Regulation of AQP2 expression, especially in the epididymal head, seemed to occur at the post-transcriptional level, as protein expression upon immunohistochemistry was pronounced, despite low transcript abundance. In epididymal tissue, AQPs likely contributed to fluid resorbtion, given their localization on the apical membrane of principal cells. Anat Rec, 296:1115Rec, 296: -1126Rec, 296: , 2013. V C 2013 Wiley Periodicals, Inc.Key words: stallion; aquaporin water channels; mRNA; protein Following completion of spermatogenesis, sperms leave the testis through the efferent ducts and enter the epididymis. During transit through the epididymis, spermatozoa undergo post-testicular maturation, including acquisition of progressive motility and the ability to undergo capacitation (Jones, 1998). These changes are due, at least in part, to changes in the composition of the epididymal luminal fluid microenvironment.

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Fibroblast Growth Factor-2 Is Expressed by the Bovine Uterus and Stimulates Interferon-τ Production in Bovine Trophectoderm

Cited by 100 publications

References 55 publications

Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Paradoxical effect of supplementary progesterone between Day 3 and Day 7 on corpus luteum function and conceptus development in cattle

Region‐Specific Expression of Aquaporin Subtypes in Equine Testis, Epididymis, and Ductus Deferens

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