Fibroblast growth factor receptors and regeneration of the eye lens

McDevitt, David S.; Brahma, S. K.; Courtois, Yves; Jeanny, Jean-Claude

doi:10.1002/(sici)1097-0177(199702)208:2<220::aid-aja9>3.0.co;2-k

Cited by 56 publications

(7 citation statements)

References 57 publications

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“…FGFR-3 protein was not specific to dorsal iris, but it was found at higher levels in the dorsal iris. The authors of that paper, however, failed to provide an explanation for the opposite gradient (more in ventral, less in dorsal iris) that they observed at day 36 after lentectomy, a stage that is considered equivalent to normal intact eye (24). In our study, we did not observe this gradient for FGFR-3 transcripts.…”

Section: Resultscontrasting

confidence: 94%

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Conservation of fibroblast growth factor function in lens regeneration

Rio‐Tsonis

Jung

Chiu

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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In urodele amphibians, lens induction during development and regeneration occurs through different pathways. During development, the lens is induced from the mutual interaction of the ectoderm and the optic vesicle, whereas after lentectomy the lens is regenerated through the transdifferentiation of the iris-pigmented epithelial cells. Given the known role of fibroblast growth factors (FGFs) during lens development, we examined whether or not the expression and the effects of exogenous FGF during urodele lens regeneration were conserved. In this paper, we describe expression of FGF-1 and its receptors, FGFR-2 (KGFR and bek variants) and FGFR-3, in newts during lens regeneration. Expression of these genes was readily observed in the dedifferentiating pigmented epithelial cells, and the levels of expression were high in the lens epithelium and the differentiating fibers and lower in the retina. These patterns of expression implied involvement of FGFs in lens regeneration. To further elucidate this function, we examined the effects of exogenous FGF-1 and FGF-4 during lens regeneration. FGF-1 or FGF-4 treatment in lentectomized eyes resulted in the induction of abnormalities reminiscent to the ones induced during lens development in transgenic mice. Effects included transformation of epithelial cells to fiber cells, double lens regeneration, and lenses with abnormal polarity. These results establish that FGF molecules are key factors in fiber differentiation, polarity, and morphogenesis of the lens during regeneration even though the regenerating lens is induced by a different mechanism than in lens development. In this sense, FGF function in lens regeneration and development should be regarded as conserved. Such conservation should help elucidate the mechanisms of lens regeneration in urodeles and its absence in higher vertebrates.

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Section: Resultscontrasting

confidence: 94%

“…FGFR-3 protein has been found to be in higher amounts in the intact dorsal than the ventral iris and during the dedifferentiation process (24). FGFR-3 protein was not specific to dorsal iris, but it was found at higher levels in the dorsal iris.…”

Section: Resultsmentioning

confidence: 96%

Conservation of fibroblast growth factor function in lens regeneration

Rio‐Tsonis

Jung

Chiu

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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“…Fgf-2 (basic Fgf) has been shown to induce chick pigmented epithelial cells to transdifferentiate into lens fibers, suggesting a conserved mechanism (Hyuga et al, 1993). There are also studies demonstrating up-regulation of Fgf receptors in lens regeneration (Del Rio- Tsonis et al, 1997;McDevitt et al, 1997;Arresta et al, 2005). Fgf signaling is, therefore, a strong candidate for initiating transdifferentiation in competent tissues, but whether or not it is indispensible for the process has yet to be determined.…”

Section: Fgf Signalingmentioning

confidence: 99%

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Beck

Belmonte

Christen

2009

Developmental Dynamics

150

139

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While Xenopus is a well-known model system for early vertebrate development, in recent years, it has also emerged as a leading model for regeneration research. As an anuran amphibian, Xenopus laevis can regenerate the larval tail and limb by means of the formation of a proliferating blastema, the lens of the eye by transdifferentiation of nearby tissues, and also exhibits a partial regeneration of the postmetamorphic froglet forelimb. With the availability of inducible transgenic techniques for Xenopus, recent experiments are beginning to address the functional role of genes in the process of regeneration. The use of soluble inhibitors has also been very successful in this model. Using the more traditional advantages of Xenopus, others are providing important lineage data on the origin of the cells that make up the tissues of the regenerate. Finally, transcriptome analyses of regenerating tissues seek to identify the genes and cellular processes that enable successful regeneration.

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“…Several FGF receptors are expressed including FGFR1, FGFR2, and FGFR3 [16,23,24]. Detailed examination of the receptors, showed that FGFR-1 is confined to the dedifferentiating dorsal iris and plays a role in regulating lens regeneration.…”

Section: Fgf Signaling Pathwaymentioning

confidence: 99%

“…Detailed examination of the receptors, showed that FGFR-1 is confined to the dedifferentiating dorsal iris and plays a role in regulating lens regeneration. McDevitt and others showed that lens regeneration could be interrupted by injecting an FGF receptor-directed mitotoxin into regenerating newt eyes [24]. In another inhibition study, newts were maintained in a solution of SU5402, a chemical that prevents autophosphorylation of FGFR-1 [23].…”

Section: Fgf Signaling Pathwaymentioning

confidence: 99%

Signaling during lens regeneration

Grogg

Call

Tsonis

2006

Seminars in Cell & Developmental Biology

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The newt is one of the few organisms that is able to undergo lens regeneration as an adult. This review will examine the signaling pathways that are involved in this amazing phenomenon. In addition to outlining the current research involved in elucidating the key signaling molecules in lens regeneration, we will also highlight some of the similarities and differences between lens regeneration and development.

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Fibroblast growth factor receptors and regeneration of the eye lens

Cited by 56 publications

References 57 publications

Conservation of fibroblast growth factor function in lens regeneration

Conservation of fibroblast growth factor function in lens regeneration

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Signaling during lens regeneration

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