Fibroblastic response to treatment with different preparations rich in growth factors

Anitua, Eduardo; Sánchez, Mikel; Zalduendo, Mar; Fuente, María de la; Prado, Roberto; Orive, Gorka; Andı́a, Isabel

doi:10.1111/j.1365-2184.2009.00583.x

Cited by 221 publications

(175 citation statements)

References 40 publications

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“…The positive effect of PRP on fibroblast proliferation has already been described by Anitua et al, 54 Creeper and Ivanovski, 55 and Passaretti et al 56 Unfortunately these teams did not use a polymeric scaffold, so it was not possible to observe the benefits from the multiplication of the positive effect on the fibroblasts from both the polymer scaffold and the PRP. Platelets were shown to release numerous GFs with mitogenic and anabolic function (eg, platelet-derived growth factor, epidermal growth factor, and transforming growth factor-beta).…”

Section: Absorbance (Au)mentioning

confidence: 91%

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Plencner

Prosecká

Rampichová³

et al. 2015

IJN

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Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2–3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

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Section: Absorbance (Au)mentioning

confidence: 91%

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Plencner

Prosecká

Rampichová³

et al. 2015

IJN

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“…This was expected, as it had previously been demonstrated that PRGF, as well as growth factors secreted by macrophages, enhanced fibroblast, mesenchymal, and stromal stem cell proliferation. 2,[33][34][35][36][37] In general, ADSC proliferation in all preconditioned medium increased from day 1 to day 4; however, by day 7 it appeared that proliferation slowed, and in some cases cell number even decreased, potentially due to induced contact inhibition as the cells became confluent in the wells, or died off following exhaustion of medium nutrients. This may also have been because the conditioned medium used for the ADSCs was MSFM, which is unfavorable over the long-term for ADSC growth, or due to harmful factors expressed during macrophage apoptosis.…”

Section: Released Prgf Effect On Cell Proliferationmentioning

confidence: 99%

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

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Platelet-rich plasma (PRP) therapy has seen a recent spike in clinical interest due to the potential that the highly concentrated platelet solutions hold for stimulating tissue repair and regeneration. The aim of this study was to incorporate PRP into a number of electrospun materials to determine how growth factors are eluted from the structures, and what effect the presence of these factors has on enhancing electrospun scaffold bioactivity. PRP underwent a freeze-thaw-freeze process to lyse platelets, followed by lyophilization to create a powdered preparation rich in growth factors (PRGF), which was subsequently added to the electrospinning process. Release of protein from scaffolds over time was quantified, along with the quantification of human macrophage and adipose-derived stem cell (ADSC) chemotaxis and proliferation. Protein assays demonstrated a sustained release of protein from PRGF-containing scaffolds at up to 35 days in culture. Scaffold bioactivity was enhanced as ADSCs demonstrated increased proliferation in the presence of PRGF, whereas macrophages demonstrated increased chemotaxis to PRGF. In conclusion, the work performed in this study demonstrated that the incorporation of PRGF into electrospun structures has a significant positive influence on the bioactivity of the scaffolds, and may prove beneficial in a number of tissue engineering applications.

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“…The latter could explain why an additional supplement with FCS is needed. In our hands, the combination of plasma and platelet proteins represents an excellent culture media for a wide range of cells including tenocytes [17], keratocytes and conjunctival fibroblasts [18], osteoblasts, and even stem cells. Such an approach will definitely avoid the use of products from animal origin and possible immunological concerns.…”

Section: Letter To the Editormentioning

confidence: 99%

The P makes the difference in plasma rich in growth factors (PRGF) technology

Anitua¹,

Sánchez²,

Prado³

et al. 2011

Platelets

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Fibroblastic response to treatment with different preparations rich in growth factors

Abstract: Optimizing composition and use of platelet-rich products is crucial to enhancing the therapeutic potential of this technology. Our data show that the biological effects of PRGF may depend on concentration of platelets and on the anatomical source of the cells.

Cited by 221 publications

References 40 publications

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

The P makes the difference in plasma rich in growth factors (PRGF) technology

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Fibroblastic response to treatment with different preparations rich in growth factors

Abstract: Optimizing composition and use of platelet-rich products is crucial to enhancing the therapeutic potential of this technology. Our data show that the biological effects of PRGF may depend on concentration of platelets and on the anatomical source of the cells.

Cited by 221 publications

References 40 publications

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-&epsilon;-caprolactone nanofibers functionalized with thrombocyte-rich solution

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-&epsilon;-caprolactone nanofibers functionalized with thrombocyte-rich solution

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

The P makes the difference in plasma rich in growth factors (PRGF) technology

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Product

Resources

About

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution