Fibroin membrane preparation and stabilization by polyethylene glycol diglycidyl ether

Abstract

Membranes prepared by drying aqueous Bombyx mori silk fibroin (SF) solution and modified silk fibroin (MSF) solutions, prepared by adding the low molecular weight crosslinking agent, polyethylene glycol diglycidyl ether (PEGDE) MW 526, 0-10% w/w, were investigated by Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, and UV-vis spectroscopy. Weight gain in aqueous solutions and their mechanical properties (tensile strength, elongation, and Young's modulus) were then characterized. SEM measurements revealed greater porosity in MSF membranes. IR spectra showed transformation from the largely a-helical/ random coil structures in SF membranes to predominantly b-sheet in MSF membranes. Results from UV-vis spectroscopy showed that the MSF membranes were largely insoluble within the pH range of 4-10. Water absorbability of the MSF membranes improved with increasing the amounts of cross-linker, up to 4%. The MSF membranes showed greater pliability and tenacity, but lower tensile strength, with increasing PEGDE concentrations. In the wet condition, PEGDE levels up to 4% can improve both tensile strength and tenacity of the MSF membrane, but higher levels (up to 10%) did not significantly change these properties.

Effects of sterilization methods on the physical, chemical, and biological properties of silk fibroin membranes

“…During freezing, the water presented in the membrane turns into ice crystals that sublimate during the lyophilization. During water sublimation, SF groups that were bonded to the water make new intra and intermolecular interactions, which helps in the membrane stability …”

“…During water sublimation, SF groups that were bonded to the water make new intra and intermolecular interactions, which helps in the membrane stability. 29 ORIGINAL RESEARCH REPORT JOURNAL OF BIOMEDICAL MATERIALS RESEARCH B: APPLIED BIOMATERIALS | MAY 2014 VOL 102B, ISSUE 4 XRD All SF dense membranes before and after sterilization showed similar XRD patterns (Figure 4-1) with halos in 20 , related to the b-sheet crystals of 4.55 Å. In addition, other less intense halos related to b-sheet were observed at 24.5 (3.8 Å), 16.5 (5.37 Å), and 8.7 (10.1 Å).…”

Role of silk fibroin and rice starch on some physical properties of hydroxyapatite‐based composites

“…SF was extracted from B. mori silk wastes (Nangnoi Srisaket 1), purchased from Queen Sirikit Sericulture Center, Chiang Mai province, Thailand. SF powder derived from silk waste was prepared as described by Moonsri et al . RS (Era‐Tab, 99%) was purchased from Erawan Pharmaceutical Research and Laboratory Co., Ltd, Thailand.…”

“…SF powder derived from silk waste was prepared as described by Moonsri et al . The HA‐based composites were prepared, as described by Sriudom et al ., in a binary medium of EtOH/H 2 O at 4 : 1 volume ratio.…”

Genipin-cross-linked silk fibroin microspheres prepared by the simple water-in-oil emulsion solvent diffusion method

“…5). The SF conformational transition from random coil to β-sheet form induced by cross-linking process has been reported [25]. The cross-linking reaction may enhance rearrangement of SF molecules.…”