Fibroma of Tendon Sheath and Tendosynovial Giant Cell Tumors are Rich in Factor XIIIa+ Dendrophages

Silverman, Jeffrey S.; Knapik, Maria

doi:10.1179/014788896794730892

Cited by 7 publications

(34 citation statements)

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“…CD68 and anti-Ki-67 antigen (Dako) was applied for 10 min at room temperature. Staining was otherwise performed as previously reported 11 . Oestrogen and progesterone receptor antibodies (Biogenex) were applied for 2 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…CD34 is involved in modulation of both proand anti-adhesive cellular behaviour and in signal transduction and regulation of cellular differentiation [6][7][8] . Multipotent CD34 dendritic cells may be histogenetically related to myofibroblasts [9][10][11] and are seen in a variety of mesenchymal tumours [9][10][11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

“…Dendritic stromal cells which express coagulation factor XIIIa are histiocytic stem cells that arise from primitive mesenchyme early in embryogenesis 11,16,17 , and persist in adult collagenous connective tissue 18 including mammary stroma. Coagulation factor XIIIa is a pro-enzyme that in vitro regulates fibroblast growth and protein synthesis 19 and that also catalyses polymerization of monomeric fibrin, fibronectin and collagen 20 .…”

Section: Introductionmentioning

confidence: 99%

“…Coagulation factor XIIIa is a pro-enzyme that in vitro regulates fibroblast growth and protein synthesis 19 and that also catalyses polymerization of monomeric fibrin, fibronectin and collagen 20 . The so-called factor XIIIa+ collagen associated dendrophages proliferate locally in wound repair and inflammatory stromal reactions 11,18,21 . Some mesenchymal tumours are rich in factor XIIIa cells 11,[22][23][24][25][26][27][28] .…”

Section: Introductionmentioning

confidence: 99%

“…The so-called factor XIIIa+ collagen associated dendrophages proliferate locally in wound repair and inflammatory stromal reactions 11,18,21 . Some mesenchymal tumours are rich in factor XIIIa cells 11,[22][23][24][25][26][27][28] . Because of their ubiquity and lack of cellular anaplasia in tumours, the neoplastic nature of factor XIIIa dendrophages in tumours in which they often abound remains controversial 11,22,26,[29][30][31] , but the deployment during embryogenesis of factor XIIIa dendritic cells bespeaks a critical role for these pleotropic stem cells in the morphogenesis of fibrovascular connective tissue and possibly in tumours derived therefrom 11 .…”

Section: Introductionmentioning

confidence: 99%

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Mammary fibroadenoma and some phyllodes tumour stroma are composed of CD34+ fibroblasts and factor XIIIa+ dendrophages

Silverman

Tamsen

1996

Histopathology

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Fibroadenomas and mammary phyllodes tumour arise by proliferation of mammary stroma and epithelial elements. However, it is the stromal element that determines the biology of these biphasic tumours. Normal mammary stroma, like most collagenous connective tissue, contains resident populations of CD34+ dendritic interstitial cells and scattered factor XIIIa+ collagen-associated dendrophages. Actin+myofibroblasts are usually absent from mammary stroma in non-disease states. To determine whether CD34+ and factor XIIIa+ cells proliferate in fibroadenomas and phyllodes tumours, and to study myofibroblastic differentiation in these lesions, we examined 19 fibroadenomas in 14 patients along with five low grade and two high grade phyllodes tumours. We employed antibodies against the human progenitor cell antigen CD34, coagulation factor XIIIa and HHF-35 actin. In three fibroadenomas and two phyllodes tumours, we used Ki-67 antigen to study cell proliferation and oestrogen and progesterone receptors to study possible hormonal influence on stromal cells. In all fibroadenomas, CD34 strongly stained interlobular, pericanalicular and intracanalicular fibroblasts with collagenous and/or myxoid features. Four low grade phyllodes tumours also had CD34+ fibroblasts as did one high grade tumour. Actin reactivity varied and was most pronounced in six fibroadenomas resembling the so-called cellular variant, while seven regular fibroadenomas had no actin+stromal cells and six had only focal and weak actin+stromal cells. Factor XIIIa+ cells were prominently admixed in the stroma of all tumours studied comprising from 5% to 20% in fibroadenomas and, focally, up to 50% in phyllodes tumours. Oestrogen and progesterone receptors were expressed only in glandular elements. Ki-67 index in stromal cells was 1% to 3% in fibroadenoma, 10% to 20% in low grade, and 20% to 40% in high grade phyllodes tumour. We conclude that fibroadenomas and some phyllodes tumours are composed of CD34+ fibroblasts that show varying myxoid, collagenous or myofibroblastic differentiation. The fibroblasts are accompanied by a subset of dendritic histiocytes that express factor XIIIa. Fibroadenoma variants show prominent collagenous actin+myofibroblastic differentiation of CD34+ stromal cells, sometimes with a gradient of CD34 down-regulation. Fine-needle or limited stereotactic core biopsy of these biphasic tumours, if they yield only stromal cells, must be distinguished from other CD34+ stromal tumours. Increased factor XIIIa+ dendrophage populations were seen in phyllodes tumours, especially in two high grade tumours that had malignant fibrous histiocytoma-like features, suggesting clonal evolution toward the fibrohistiocytic final pathway. Further study of CD34 and factor XIIIa+ mammary stromal cells in larger numbers of phyllodes tumours might ascertain whether increasing factor XIIIa reactivity correlates with differentiation and increased tumour aggressiveness.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mammary fibroadenoma and some phyllodes tumour stroma are composed of CD34+ fibroblasts and factor XIIIa+ dendrophages

Silverman

Tamsen

1996

Histopathology

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Immunohistological analysis of transglutaminase factor XIIIA expression in mouse embryonic growth plate

Nurminskaya

Linsenmayer

2002

Journal Orthopaedic Research

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Previously we demonstrated the expression of Factor XIIIA (FXIIIA), a coagulatioli traasglutaminase, in avian embryonic growth plate. To explore whether FXIIIA is also expressed by chondrocytes of the iiiamliialian cartilage anlagen of bones, we analyzed tlie iiiouse embryonic growth plate by iiiiinuiiostainilig using anti-FXIIIA antibodies developed against human zind chicken proteins. We revealed the expression of FXIIIA in the epipliyseal growth plate, where FXIIIA appears first intracellularly in the zone of proliferation/iiiaturatioii, and remains intra-and extracellularly throughout the hypertrophic zone. Externalizatioii of FXIIIA O C C L I~S before mineralization. Transglutaminase activity was assayed in organ cultures using rliodamine-labeled synthetic substrate Pro-Val-Lys-Gly. Enzyiiiatic activity shows a restricted distribution in cartilage and correlates with FXIIIA expression pattern, suggesting that cartilagenous transglutaminase activity is due, at least partially, to the FXIIIA isoform. We conclude, that coagulation factor FXIIIA is expressed by chondrocytes of embryonic mouse long bone cartilages in a strictly regulated pattern.which correlates with chondrocyte differentiation and matrix mineralization.

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Cardiac myxoma is rich in factor XIIIa positive dendrophages: immunohistochemical study of four cases

Berrutti

Silverman

1996

Histopathology

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Cardiac myxoma is an enigmatic tumour thought to arise from primitive cardiac mesenchymal cells. Factor XIIIa+ dendrophages are tissue histiocytes that are active in tissue repair and thrombosis. To explore whether factor XIIIa+ dendrophages play a role in cardiac myxoma morphogenesis, we stained four cases with an antiserum against coagulation factor XIIIa (FXIIIa). We also used antibodies recognizing CD34, CD31, and S-100 protein. Samples of valvular endocardium from 12 and 16 week fetuses and two adult autopsies were compared with the four myxomas. All cardiac myxomas had rounded and dendritic FXIIIa+ cells admixed with more numerous CD34+ spindle and stellate myxoma cells. The CD34+ cells formed multicellular syncytia and capillary sprouts. Many of these syncytial structures also expressed CD31 and, to a lesser extent, S-100 protein, strongly in two cases and more focally in two. Fetal subendocardium was composed of CD34+ stellate fibroblast-like cells invested with scattered FXIIIa+ histiocytes; no S-100+ cells were detected. Our findings confirm that cardiac myxomas are composed of CD34+ primitive subendocardial cells. These cells show a capacity for CD31+ endothelial differentiation. In cardiac myxoma, the CD34+ myxoma cells are accompanied by numerous FXIIIa+ dendrophages, the presence of which suggests abnormal organizing thrombus-like differentiation in cardiac myxoma morphogenesis.

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Fibroma of Tendon Sheath and Tendosynovial Giant Cell Tumors are Rich in Factor XIIIa+ Dendrophages

Cited by 7 publications

References 0 publications

Mammary fibroadenoma and some phyllodes tumour stroma are composed of CD34+ fibroblasts and factor XIIIa+ dendrophages

Mammary fibroadenoma and some phyllodes tumour stroma are composed of CD34+ fibroblasts and factor XIIIa+ dendrophages

Immunohistological analysis of transglutaminase factor XIIIA expression in mouse embryonic growth plate

Cardiac myxoma is rich in factor XIIIa positive dendrophages: immunohistochemical study of four cases

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