Fibronectin‐derived Epiviosamines enhance PDGF‐BB‐stimulated human dermal fibroblast migration<i>in vitro</i>and granulation tissue formation in vivo

Prasad, Atulya; Lin, Fubao; Clark, Richard A.F.

doi:10.1111/wrr.12744

Cited by 7 publications

(5 citation statements)

References 67 publications

(260 reference statements)

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“…The proliferation stage mainly refers to the process of granulation tissue formation, angiogenesis, and re-epithelialization [28,29]. Under the action of growth factors produced by platelets and macrophages, fibroblasts proliferate and secrete collagen, and increase the amount of ECM components in the wound, thus promoting granulation tissue formation [30].…”

Section: Proliferation Stagementioning

confidence: 99%

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

et al. 2021

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Diabetic wounds are complications of diabetes which are caused by skin dystrophy because of local ischemia and hypoxia. Diabetes causes wounds in a pathological state of inflammation, resulting in delayed wound healing. The structure of electrospun nanofibers is similar to that of the extracellular matrix (ECM), which is conducive to the attachment, growth, and migration of fibroblasts, thus favoring the formation of new skin tissue at the wound. The composition and size of electrospun nanofiber membranes can be easily adjusted, and the controlled release of loaded drugs can be realized by regulating the fiber structure. The porous structure of the fiber membrane is beneficial to gas exchange and exudate absorption at the wound, and the fiber surface can be easily modified to give it function. Electrospun fibers can be used as wound dressing and have great application potential in the treatment of diabetic wounds. In this study, the applications of polymer electrospun fibers, nanoparticle-loaded electrospun fibers, drug-loaded electrospun fibers, and cell-loaded electrospun fibers, in the treatment of diabetic wounds were reviewed, and provide new ideas for the effective treatment of diabetic wounds.

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Section: Proliferation Stagementioning

confidence: 99%

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

et al. 2021

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“…96 Certain FN peptides incorporated in hyaluronan hydrogel stimulate the formation of granulation tissue in excisional wounds in the skin of pigs. 97,98…”

Section: Fibronectinmentioning

confidence: 99%

“…96 Certain FN peptides incorporated in hyaluronan hydrogel stimulate the formation of granulation tissue in excisional wounds in the skin of pigs. 97,98 FN is a glycoprotein that contains oligosaccharide chains on asparagin residues (N-glycosylation sites) and on threonine residues (O-glycosylation sites). Glycosylation modifies the function of FN domains and is different in pFN and cFN.…”

Section: Prospects For the Use Of Fibronectin In The Treatment Of Chr...mentioning

confidence: 99%

Role of fibronectin in chronic venous diseases: A review

Kanta

Zavaďáková

2020

Vasc Med

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Fibronectin (FN) circulating in the blood and produced by cells provides the basis of the extracellular matrix (ECM) formed in healing acute wounds. The time-dependent deposition of FN by macrophages, its synthesis by fibroblasts and myofibroblasts, and later degradation in the remodeled granulation tissue are a prerequisite for successful healing of wounds. However, the pattern of FN expression and deposition in skin lesions is disturbed. The degradation of the ECM components including FN in varicose veins prevails over ECM synthesis and deposition. FN is inconspicuous in the fibrotic lesions in lipodermatosclerosis, while tenascin-C containing FN-like peptide sequences are prominent. FN is produced in large amounts by fibroblasts at the edge of venous ulcers but FN deposition at the wound bed is impaired. Both the proteolytic environment in the wounds and the changed function of the ulcer fibroblasts may be responsible for the poor healing of venous ulcers. The aim of this review is to describe the current knowledge of FN pathophysiology in chronic venous diseases. In view of the fact that FN plays a crucial role in organizing the ECM, further research focused on FN metabolism in venous diseases may bring results applicable to the treatment of the diseases.

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“…Therefore, the dynamic balance of migration, proliferation, and apoptosis among immune cells, fibroblasts, and endothelial cells is of great significance for wound healing. Fibroblasts are the most important functional cells in the wound healing process and participate in the regulation of inflammation and secretion of the ECM to form granulation tissue 6 . At the early stage of wound healing, fibroblasts can directly interact with neutrophils, and fibroblasts release cytokines to recruit leukocytes and stimulate their activation, which in turn inhibits wound inflammation 7,8 .…”

Section: Introductionmentioning

confidence: 99%

“…Fibroblasts are the most important functional cells in the wound healing process and participate in the regulation of inflammation and secretion of the ECM to form granulation tissue. 6 At the early stage of wound healing, fibroblasts can directly interact with neutrophils, and fibroblasts release cytokines to recruit leukocytes and stimulate their activation, which in turn inhibits wound inflammation. 7,8 In the middle and late stages of wound healing, fibroblasts are the dominant cells interacting with keratinocytes, causing the microenvironment to gradually shift from the inflammatory to the granulation phase with the synthesis of new tissue.…”

mentioning

confidence: 99%

Role of haematopoietic cell‐specific protein 1‐associated protein X‐1 gene in lipopolysaccharide‐induced apoptosis of human dermal fibroblasts

Ning

Chen

et al. 2021

Wound Repair Regeneration

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Wound healing may be disrupted by lipopolysaccharide (LPS)‐induced mitochondrial dysfunction, inflammation, and excessive oxidative stress, which can lead to undesirable consequences. The haematopoietic cell‐specific protein 1‐associated protein X‐1 (HAX‐1) is a mitochondrial matrix protein that regulates mitochondrial function. This study aimed to comprehensively identify the role of HAX‐1 in the inhibition of LPS‐induced mitochondrial dysfunction and apoptosis in human dermal fibroblasts (HDFs). HAX‐1 expression was assessed in the HDF‐a cell line using real‐time polymerase chain reaction, western blotting, and immunohistochemical staining. The viability, migration, and apoptosis of HDF‐a cells were evaluated using the water‐soluble tetrazolium‐1 assay, transwell assay, and flow cytometry analysis, respectively. Mitochondrial function was evaluated based on reactive oxygen species (ROS) generation and mitochondrial membrane potential (ΔΨm). Our results demonstrated that LPS stimulation markedly repressed HAX‐1 expression in HDFs and silencing of HAX‐1 led to mitochondrial ROS accumulation, ΔΨm disruption, and abnormal mitochondrial morphology. Accordingly, overexpression of HAX‐1 or administration of metformin enhanced mitochondrial fusion and normalized mitochondrial dynamics, thereby reversing LPS‐induced mitochondrial dysfunction, fibroblast apoptosis, and viability and migration inhibition in HDF‐a cells. These data support a mechanism wherein HAX‐1 plays a crucial role in LPS‐induced fibroblast apoptosis in a mitochondria‐dependent manner.

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Fibronectin‐derived Epiviosamines enhance PDGF‐BB‐stimulated human dermal fibroblast migrationin vitroand granulation tissue formation in vivo

Cited by 7 publications

References 67 publications

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

Role of fibronectin in chronic venous diseases: A review

Role of haematopoietic cell‐specific protein 1‐associated protein X‐1 gene in lipopolysaccharide‐induced apoptosis of human dermal fibroblasts

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