Fibronectin Enhances In Vitro Lipopolysaccharide Priming of Polymorphonuclear Leukocytes

Bortolussi, Robert; Rajaraman, K.; Qing, Gefei; Rajaraman, R.

doi:10.1182/blood.v89.11.4182

Cited by 15 publications

(7 citation statements)

References 46 publications

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“…Fibronectin, in combination with other plasma factors, has been demonstrated to enhance the sensitivity of polymorphonuclear leukocytes to LPS (Bortolussi et al. 1997).…”

Section: Discussionmentioning

confidence: 99%

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Fibronectin is elevated in the cerebrospinal fluid of patients suffering from bacterial meningitis and enhances inflammation caused by bacterial products in primary mouse microglial cell cultures

Goos

Lange

Hanisch

et al. 2007

Journal of Neurochemistry

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Toll-like receptors (TLR) play a key role in the recognition of pathogenic organisms. Fibronectin, an extracellular matrix protein, is considered a potent stimulator of the innate immune system through TLR4. In bacterial meningitis, several extracellular matrix proteins and bacterial compounds are elevated in the CSF. For this reason, we hypothesized that these molecules may jointly stimulate the innate immune system and increase neuronal damage in bacterial meningitis. Concentrations of fibronectin were elevated in the CSF of patients suffering from bacterial meningitis, but not in patients with multiple sclerosis, when compared with control patients without CSF abnormalities. In primary cultures of mouse microglial cells, co-administration of fibronectin at concentrations occurring in the CSF in bacterial meningitis (10 microg/mL) with defined TLR agonists [lipopolysaccharide (TLR4), the synthetic lipopeptide tripalmytoyl-cysteinyl-seryl-(lysyl)3-lysine (TLR2) and single-stranded unmethylated cytosine-guanosine oligodesoxynucleotide (TLR9)] led to an additive release of nitric oxide and tumor necrosis factor-alpha when compared with the release elicited by either compound alone. In conclusion, the inflammatory reaction to bacterial compounds can be aggravated by endogenous fibronectin at elevated levels during bacterial CNS infections. This additive or synergistic effect may contribute to neuronal damage during bacterial meningitis.

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“…Fibronectin, in combination with other plasma factors, has been demonstrated to enhance the sensitivity of polymorphonuclear leukocytes to LPS (Bortolussi et al. 1997).…”

Section: Discussionmentioning

confidence: 99%

“…More specific approaches to antagonize the effect of fibronectin include the application of monoclonal antibodies against fibronectin or its receptors, the β1 integrins (Mandy et al. 1994; Bortolussi et al. 1997; Vaday et al.…”

Section: Discussionmentioning

confidence: 99%

Fibronectin is elevated in the cerebrospinal fluid of patients suffering from bacterial meningitis and enhances inflammation caused by bacterial products in primary mouse microglial cell cultures

Goos

Lange

Hanisch

et al. 2007

Journal of Neurochemistry

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“…Activated monocytes liberate early the cytokines TNF‐α and interleukin (IL)‐1 and, together with other factors (13), prime and sensitize neutrophils to the effects of other agonists. Preoperative immune activation may be due to congenital heart disease (14, 15) or to subclinical inflammation (9), or it may result from an atopic/allergic predisposition (16, 17).…”

Section: Discussionmentioning

confidence: 99%

“…It is desirable to predict postoperative complications, induced by an activated immune system (1, 9, 13), from a predisposition by the cardiac disease (14, 15) or from other causes (e.g., atopy/allergy; 16,17) already present preoperatively (7). Predisposed patients may respond more dramatically to CPB and surgical trauma.…”

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confidence: 99%

Preoperative prediction of postoperative edema and effusion in pediatric cardiac surgery by altered antigen expression patterns on granulocytes and monocytes

et al. 2001

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Postoperative edema and effusion (POEE) following cardiopulmonary bypass (CPB) surgery in children retards recovery and may aggravate postpericardiotomy (PPS), capillary leak syndrome (CLS), or multiorgan failure (MOF). Compared with complication-free children, POEE affected children have different preoperative serum levels of circulating cytokines and adhesion molecules. These levels may be used preoperatively to assess POEE, but their determination is time consuming, costly, and a substantial blood volume is required. Altered serum levels of cytokines and adhesion molecules also may be reflected in altered antigen expression on circulating blood leukocytes. The predictive potential of flow cytometric (FCM) leukocyte immunophenotyping was explored as a sensitive and fast method that required small blood samples. Blood samples taken 24 h preoperatively from 49 patients (3-18 years old) were stained with monoclonal antibodies for adhesion molecules (ICAM-1, LFA-1, Mac-1) or constitutive/activation markers (CD4, CD14, CD16, CD25, CD54, CD69, HLA-DR) and measured on a microbead calibrated FCM. Neutrophils, monocytes, and eosinophils from POEE patients express higher preoperative levels of LFA-1, monocytes, HLA-DR, and other activation markers (all P < 0.03). Over 89% of the patients were classified correctly by using two discriminant analysis methods (sensitivity, >76%; specificity, >86%; positive prediction, >80%; negative prediction, >83%). Granulocytes and monocytes of postoperative POEE patients exhibit significant preoperative immune activation, suggesting an increased risk for patients with atopic/allergic predisposition. Surgical trauma and CPB cause additional immune activation, leading to POEE by a summative response. Most patients at risk for POEE can be identified preoperatively by using data pattern analysis on FCM-derived parameters. Cytometry (Comm. Clin. Cytometry) 46:247-253, 2001.

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“…hyaluronan secreted more cytokine than cells treated with LPS alone [106]. Fibrinogen and fibronectin were shown to prime neutrophils toward greater responsiveness to LPS [108,104], and fibronectin was shown additionally to enhance microglial cell responses to lipopeptide and CpG DNA [105]. Mice genetically deficient in biglycan were shown to be considerably less responsive to LPS than wildtype animals [35] in a manner similar to that shown for the related LRR-containing ECM component lumican [97].…”

Section: Evidence That Damps May Serve As Psmsmentioning

confidence: 99%

Endogenous ligands of TLR2 and TLR4: agonists or assistants?

Erridge

2010

Journal of Leukocyte Biology

470

415

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The mammalian TLRs serve as key sensors of PAMPs, such as bacterial LPS, lipopeptides, and flagellins, which are present in microbial cells but not host cells. TLRs have therefore been considered to play a central role in the discrimination between "self" and "non-self". However, since the discovery of their microbial ligands, many studies have provided evidence that host-derived molecules may also stimulate TLR2- or TLR4-dependent signaling. To date, more than 20 of these endogenous TLR ligands have been proposed, which have tended to fall into the categories of released intracellular proteins, ECM components, oxidatively modified lipids, and other soluble mediators. This review aims to summarize the evidence supporting the intrinsic TLR-stimulating capacity of each of these proposed endogenous ligands with a particular emphasis on the measures taken to exclude contaminating LPS and lipopeptides from experimental systems. The emerging evidence that many of these molecules may be more accurately described as PAMP-binding molecules (PBMs) or PAMP-sensitizing molecules (PSMs), rather than genuine ligands of TLR2 or TLR4, is also summarized. The relevance of this possibility to the pathogenesis of chronic inflammatory diseases, tumor surveillance, and autoimmunity is discussed.

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Fibronectin Enhances In Vitro Lipopolysaccharide Priming of Polymorphonuclear Leukocytes

Cited by 15 publications

References 46 publications

Fibronectin is elevated in the cerebrospinal fluid of patients suffering from bacterial meningitis and enhances inflammation caused by bacterial products in primary mouse microglial cell cultures

Fibronectin is elevated in the cerebrospinal fluid of patients suffering from bacterial meningitis and enhances inflammation caused by bacterial products in primary mouse microglial cell cultures

Preoperative prediction of postoperative edema and effusion in pediatric cardiac surgery by altered antigen expression patterns on granulocytes and monocytes

Endogenous ligands of TLR2 and TLR4: agonists or assistants?

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