Fibronectin-plasma membrane interaction in the adhesion of hemopoietic cells.

Giancotti, Filippo G.; Comoglio, Paolo M.; Tarone, Guido

doi:10.1083/jcb.103.2.429

Cited by 108 publications

(31 citation statements)

References 43 publications

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“…In contrast, the functional requirements of the plasma membrane of the committed erythroid progenitor are vastly different. These progenitors engage in cell-cell and cell-extracellular matrix adhesion through expression of receptors for matrix components, such as the fibronectin receptors, VLA-4 and VLA-5, and a sialyated macrophage receptor (27)(28)(29)(30)(31)(32). In addition, the progenitor membrane facilitates endocytosis with, for example, transferrin receptor recycling (33-35), while the membrane of mature red cells does not undergo extensive receptor-mediated endocytosis.…”

Section: Discussionmentioning

confidence: 99%

Differentiation-associated switches in protein 4.1 expression. Synthesis of multiple structural isoforms during normal human erythropoiesis.

Chasis¹,

Coulombel²,

Conboy³

et al. 1993

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Differentiation-associated switches in protein 4.1 expression. Synthesis of multiple structural isoforms during normal human erythropoiesis.

Chasis¹,

Coulombel²,

Conboy³

et al. 1993

J. Clin. Invest.

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“…For example, adhesive properties have been used to distinguish hemopoietic cells with different spleen colony-forming and marrow-repopulating abilities (Bearpark and Gordon 1989). Immature myeloid and erythroid cells have been shown to bind to both fibronectin (Giancotti et al 1986) and collagen Type I (Tsai et al 1987;Koenigsmann et al 1992). Human CD34 ϩ progenitors bind to fibronectin in an activation-dependent manner (Kerst et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Immunofluorescence Characterization of Key Extracellular Matrix Proteins in Murine Bone Marrow In Situ

Nilsson

Debatis

Dooner

et al. 1998

J Histochem Cytochem.

156

134

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SUMMARYThe mechanism of hemopoietic stem cell homing to the bone marrow involves molecular interactions that mediate the recognition and interaction of these cells with the marrow microenvironment, including the extracellular matrix. On selective binding, this environment, in combination with soluble cytokines, regulates stem cell proliferation and differentiation. Using immunofluorescence labeling, we analyzed the location of the prominent extracellular matrix proteins fibronectin, collagen Types I, III, and IV, and laminin in sections of murine femoral bone marrow. Collagen Types I, IV, and fibronectin were localized to the endosteum, the region of the femoral microenvironment for which homing stem cells have a high affinity. The results further demonstrated a strong spatial association of collagen Type IV and laminin with the bone marrow vessels, including arterioles, veins, and sinuses. Fibronectin was distributed throughout the central marrow region, and all the proteins analyzed except collagen Type III were present in the bone, although at different levels. Fibronectin, collagen Types III and IV, and laminin were also present in the periosteum. The distinct locations of particular extracellular matrix proteins support the notion that they may play an important mechanistic role in the homing of engrafting cells. (J Histochem Cytochem 46:371-377, 1998)

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“…This was first demonstrated for erythroid cells (Patel & Lodish, 1984) and subsequently for myeloid cells (Giancotti et al 1986) and is mediated via a wellcharacterized 140K (K = 10 3 M r ) receptor on immature haematopoietic cells, which is likely to belong to the integrin family of cell adhesion molecules (Hynes, 1987). Interestingly, studies in the mouse and human have shown that adhesion of erythroleukaemia cells or erythroblasts to fibronectin is able to promote haemoglobinization, terminal differentiation and enucleation in the presence of chemical inducers or erythropoietin Tsai et al 1987).…”

Section: Importance Of Cell-cell Contact In Haematopoiesismentioning

confidence: 99%

Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells

Crocker

Morris

Gordon

1988

Journal of Cell Science

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SummaryImmunocytochemical staining of tissues with the mouse macrophage-specific monoclonal antibody, F4/80, has shown that large numbers of stromal macrophages are present in adult and foetal haematopoietic tissues. Macrophage plasma membrane processes are seen to establish extensive associations with myeloid and erythroid cells in adult bone marrow and with developing erythroblasts in foetal liver, suggestive of local trophic interactions. To explore the nature of these interactions, methods were developed for isolation of resident bone marrow macrophages (RBMM) and foetal liver macrophages (FLM). Following collagenase digestion of bone marrow or foetal liver, clusters were obtained which were composed of one or more central macrophages surrounded by proliferating haematopoietic cells. After attachment of clusters to glass coverslips, adherent macrophages could be stripped free of haematopoietic cells by pipetting in the absence of divalent cations. The purified RBMM, but not FLM, expressed a novel haemagglutinin, which mediated binding, without ingestion, of large numbers of unopsonized sheep erythrocytes by a divalent cation-independent mechanism. In view of the possibility that this sheep erythrocyte receptor (SER) could interact with a homologous ligand on mouse bone marrow cells, its properties were examined. SER was found to be a lectin-like protein which recognized protease-resistant sialylated glycoconjugates on sheep erythrocytes. The expression of SER was restricted to certain stromal tissue macrophages and was low or absent on monocytes and macrophages obtained from serous cavities. High levels of SER could be induced on elicited peritoneal macrophages by cultivation in mouse serum and the induced receptor was found to mediate low-avidity binding of murine bone marrow cells with characteristics indistinguishable from those seen for binding of sheep erythrocytes. However, maximal binding of bone marrow cells to RBMM depended on a distinct, divalent cation-dependent adhesion system. Using erythroblasts as a ligand, FLM were selected to explore the properties and expression of this adhesion receptor, the erythroblast receptor (EbR). Similar to SER, EbR did not mediate ingestion, and was restricted in its expression to foetal and adult stromal tissue macrophages. Unlike SER, EbR activity was not affected by neuraminidase treatment of the ligand and the receptor was not induced on peritoneal macrophages cultured in mouse serum. EbR appears to be a novel cell adhesion receptor because it was unaffected by inhibitors of several previously described cell adhesion molecules, including the fibronectin receptor. Future studies will attempt to explore the functional significance of these two receptors in macrophage-haematopoietic cell interactions.

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Fibronectin-plasma membrane interaction in the adhesion of hemopoietic cells.

Cited by 108 publications

References 43 publications

Differentiation-associated switches in protein 4.1 expression. Synthesis of multiple structural isoforms during normal human erythropoiesis.

Differentiation-associated switches in protein 4.1 expression. Synthesis of multiple structural isoforms during normal human erythropoiesis.

Immunofluorescence Characterization of Key Extracellular Matrix Proteins in Murine Bone Marrow In Situ

Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells

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