Fibrosis and Collagen-I Accumulation in Bali Cattle Liver Tissue Infected with Fasciola gigantica

Abstract

This study aims to determine the histopathological changes of fibrosis and accumulation of collagen I in liver tissue of Bali cattle infected with Fasciola gigantica in the Badung district, Bali Indonesia. On examination of 100 Bali cattle livers at the traditional abattoir of Badung, 37 of them were found to contain F. gigantica worms in both the bladder and bile ducts. The liver tissue was then fixed with 10% formalin for processing and stained with hematoxylin-eosin dyes. The liver sample preparation in the coating slide was also reacted with rabbit anti-collagen-I antibody. On histopathological examination, it was found that the distribution of fibrosis varied in the portal, interlobular, and bridging fibrosis areas. Immunohistochemical examination showed an immunoreactive reaction using rabbit anti-collagen I in the portal area with strong and weak intensity in the interlobular area and in bridging fibrosis. Fibrosis and accumulation of collagen I in Bali cattle infected with F. gigantica at the traditional abattoir of Badung district, Bali Indonesia was found in the portal, interlobular, and bridging fibrosis areas.