Fibrotic lesions in the testis of bulls and relationship to semen quality

Barth, A.D.; Alisio, Leonel; Aviles, M.; Arteaga, A; Campbell, John R.; Hendrick, S.

doi:10.1016/j.anireprosci.2007.05.002

Cited by 33 publications

(49 citation statements)

References 17 publications

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“…In summary, each testicle had 30 areas of measurement (six in the vertical plane, four in the horizontal plane, repeated separately three times). A gross visual scoring of fibrotic lesions within the testicular parenchyma was carried out to give a gross testicular fibrosis score [18]. This used a six-point scale of fibrosis per image, with 0 indicating a normal homogenous echotexture throughout the testicular parenchyma and five indicating severe fibrosis throughout the testicle (Fig.…”

Section: Methodsmentioning

confidence: 99%

The value of trans-scrotal ultrasonography at bull breeding soundness evaluation (BBSE): The relationship between testicular parenchymal pixel intensity and semen quality

et al. 2017

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Bull breeding soundness evaluation (BBSE) is commonly undertaken to identify bulls that are potentially unfit for use as breeding sires. Various studies worldwide have found that approximately 20% of the bulls fail their routine prebreeding BBSE and are therefore considered subfertile. Multiple articles describe the use of testicular ultrasound as a noninvasive aid in the identification of specific testicular and epididymal lesions. Two previous studies have hypothesized a correlation between ultrasonographic testicular parenchymal pixel intensity (PI) and semen quality; however to date, no published studies have specifically examined this link. The aim of this study, therefore, was to assess the relationship between testicular parenchymal PI (measured using trans-scrotal ultrasonography) and semen quality (measured at BBSE), and the usefulness of testicular ultrasonography as an aid in predicting future fertility in bulls, in particular those that are deemed subfertile at the first examination. A total of 162 bulls from 35 farms in the South East of Scotland were submitted to routine BBSE and testicular ultrasonography between March and May 2014, and March and May 2015. Thirty-three animals failed their initial examination (BBSE1) due to poor semen quality, and were re-examined (BBSE2) 6 to 8 weeks later. Computer-aided image analysis and gross visual lesion scoring were performed on all ultrasonograms, and results were compared to semen quality at BBSE1 and BBSE2. The PI measurements were practical and repeatable in a field setting, and although the results of this study did not highlight any biological correlation between semen quality at BBSE1 or BBSE2 and testicular PI, it did identify that gross visual lesion scoring of testicular images is comparable to computer analysis of PI (P < 0.001) in identifying animals suffering from gross testicular fibrosis.

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Section: Methodsmentioning

confidence: 99%

The value of trans-scrotal ultrasonography at bull breeding soundness evaluation (BBSE): The relationship between testicular parenchymal pixel intensity and semen quality

et al. 2017

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“…Barth et al [16] observed no difference in the fibrosis score between the left and right testicles. These same authors, when evaluating 175 bulls (Bos taurus taurus), aged between 10 and 12 months old, observed that 70% presented some testicular fibrosis impairment; of these, 15.4% had moderate to severe fibrosis, with compromised the spermatic quality.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, Barth et al [16] noted that even bulls with very severe degrees of fibrosis produced semen with up to 94% morphologically normal spermatozoa. These results indicate that the presence of relatively large amounts of scar tissue within the testicular parenchyma does not necessarily prevent the production of normal spermatozoa by other areas of the testicular parenchyma.…”

Section: Discussionmentioning

confidence: 99%

Lack of Relationship between Testicular Echotexture and Seminal Characteristics in Adult Texel Rams

Urt¹,

Bakarji²,

Faria³

et al. 2018

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The objective of this study was to determine the minimum representative area to evaluate testicular echotexture parenchyma and to identify the correlations between the intensity of pixels of the testicular parenchyma and the fibrosis score with the physical and morphological characteristics of the ejaculate of Texel rams. The study used 88 Texel rams, aged between 10 and 12 months, and reared in a semi-extensive system. The animals underwent breeding soundness evaluation (BSE) and ultrasound of the testicles. The images were transferred to a computer where they were defined in areas of 400, 1600, 3600, and 6400 mm 2 , after which the average intensity of the pixels of each image of the testicular regions was evaluated. A testicular fibrosis score was assigned in order to quantify the frequency of fibrotic lesions. In relation to the intensity of pixels of the predetermined regions, only the area of 400 mm 2 presented a difference (P < 0.05), with the area of 1600 mm 2 being the smallest area that best represented the testicular parenchyma. There were no correlations between the intensity of pixels of the testicular parenchyma and the fibrosis score with the physical and morphological characteristics of the ejaculate of the rams.

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“…Previous studies have attempted to correlate specific ultrasound features with testicular pathologies (Eilts and Pechman 1988), testicular degeneration due to insulation (Brito et al 2003, Arteaga et al 2005, daily sperm production and ejaculate quality (Kastelic et al 2001), testicular development (Silva et al 1997), and fibrotic injuries in the testicular parenchyma (Barth et al 2008). However, ultrasonography still has limited utility as a diagnostic tool in andrological examinations, most likely because research has shown little correlation between clinical results and testicular parenchyma echogenicity Pechman 1988, Brito et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

Dias

Faria

Fernandes

et al. 2017

Austral j. vet. sci.

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ABSTRACT. The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm 2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.Key words: testicular parenchyma, morphometry, ultrasonography, bovine. RESUMEN.El objetivo de este estudio fue evaluar la ecotextura testicular de los toros Nelore y correlacionarlo con los parámetros de espermatogénesis. Para este propósito, 41 toros de edades de 3-5 años y con pesos corporales en el intervalo de 510,2 ± 21,61 kg fueron seleccionados para el estudio. Los toros se sometieron a ecografía, utilizando un dispositivo portátil junto con un transductor lineal a una frecuencia de 7,5 MHz. Inmediatamente después, los animales fueron castrados, y el tejido testicular se trató con Karnovsky fijador. Los fragmentos testiculares se incluyeron en metacrilato de glicol y se tiñeron con una solución de borato de sodio-azul de toluidina al 1%. La intensidad media de pixeles en el parénquima testicular se determinó para secciones de superficie de 1.600 mm 2 . Los El área entre los dos testículos derecho e izquierdo dieron lugar a la intensidad media de los pixeles de 105,22 ± 13,58, 107,95 ± 12,82 y 106,59 ± 11,43, respectivamente. El coeficiente de eficiencia de la mitosis espermatogonial fue 19,74; Índice meiótico fue 2,63, y el rendimiento global del espermatogénesis alcanzó el 52,0. No se observó correlación (P>0,05) entre ecotextura testicular y cualquiera de los parámetros morfométricos medidos. Estos resultados sugieren que ecotextura testicular no es un método viable para el análisis indirecto de la población celular en el epitelio seminífero, el rendimiento de la espermatogénesis intrínseco, o el índice de células de Sertoli en toros Nelore.

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Fibrotic lesions in the testis of bulls and relationship to semen quality

Cited by 33 publications

References 17 publications

The value of trans-scrotal ultrasonography at bull breeding soundness evaluation (BBSE): The relationship between testicular parenchymal pixel intensity and semen quality

The value of trans-scrotal ultrasonography at bull breeding soundness evaluation (BBSE): The relationship between testicular parenchymal pixel intensity and semen quality

Lack of Relationship between Testicular Echotexture and Seminal Characteristics in Adult Texel Rams

Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

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