Fibrous architecture of cementodentinal junction in disease: A scanning electron microscopic study

Abstract

Background:The cementodentinal junction (CDJ) forms a biological and structural link between cementum and dentin. This biological link is regarded as a distinct tissue in its own right. Certain important proteins responsible for periodontal regeneration are said to be present in this tissue. Few studies have described the structure and composition of this layer by light and electron microscopy. Scanning electron microscopic studies pertaining to CDJ in health and disease are few and documentation of periodontal pathological changes of CDJ is unclear. In the first phase of our study, the collagenous architecture of CDJ of healthy teeth has been reported.Aim:The objective of this study is to observe and report periodontal pathological changes in the fibrous or collagenous architecture of CDJ of periodontitis-affected teeth and discuss the probable clinical implications of CDJ in disease.Materials and Methods:Twenty periodontitis-affected teeth were collected and processed for observing under a scanning electron microscope.Results:The results are as follows: Increased width of interface at CDJ in periodontitis samples (7.1 μ) compared to that of healthy samples; fewer areas of fiber intermingling at CDJ in periodontitis samples as compared to healthy samples; frequent detachment of cementum from dentin during sodium hydroxide maceration of samples.Conclusion:It may be inferred from results that there is a possibility of a definite weakening of CDJ in periodontally affected root surfaces and we believe that clinical procedures such as scaling and root planning may have a detrimental effect on the cementodentinal attachment of periodontally involved root surfaces.

The Structure of Cemento-dentinal Junction in Mandibular Deciduous Second Molars and Permanent First Molars in Egyptian Populations (Scanning Electron Microscope Study)

“…The fibril intermingling between the root dentin and cementum was described as point-like processes and occurred only in places at the cement-dentinal interface. No detachment of the cementodentinal junction was reported, unless the specimens were treated by NaOH-maceration method, and it is reported that NaOH-maceration method does not damage collagen fibril structure and architecture [9,13] .…”

“…The average width of the cemento-dentinal junction in the deciduous 2 nd molar may be relatively higher than that of permanent which may be related to the difference between the structure and chemical composition of dentin and cementum between the deciduous and permanent teeth. Also, Sudhakar and Pratebha, 2015 [13] stated that the cement-dentinal junction is rich in unmineralized collagen which gets easily denatured and degraded due to any physiological or pathological changes and any loss of these collagen fibers and other proteins, that may lead to increase in the width of the cement-dentinal junction and its subsequent weakening.…”

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

“…Cementum is composed of two layers of cellular and acellular cementum [ 32 ]. The interface between cementum and dentine is known as the cemento-dentinal junction (CDJ) [ 33 ]. This complex hierarchical structure of the tooth provides a rich source of insights and ideas for the development of new materials via biomimetic research [ 34 ].…”