Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats

Haslan, Muhammad Aliff; Samsulrizal, Nurdiana; Zin, Noor Syaffinaz Noor Mohamad; Shirazi, Farshad H.; Goh, Yong Meng

doi:10.1186/s12906-021-03452-6

Cited by 22 publications

(13 citation statements)

References 59 publications

(42 reference statements)

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“…Therefore, a new treatment strategy with less side effects, more affordable, and with more pleiotropic activity is required. Previous studies have documented the efficacy of several herbal medications such as resveratrol, 15 quercetin, 16 thymoquinone 17 and Ficus deltoidea 18 in restoring function of the ovaries, insulin sensitivity and hormonal profile in experimentally induced PCOS. These studies suggested that the herbal medicines with ovulatory and hypoglycemic effects might be considered as an alternative therapy for PCOS.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Marouf¹,

Ismaeel²,

Hassan³

et al. 2022

JIR

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Background Current therapies for polycystic ovary syndrome (PCOS) are accompanied by unwanted effects. Silibinin; a flavonolignan has pleiotropic activities and favorable safety profile. Purpose To investigate the efficacy of silibinin on estrous cyclicity, inflammation, oxidative stress and ovarian morphology in letrozole-induced PCOS in rats. Methods Forty-eight female Wistar albino rats were divided into 2 sets. Rats of the first set (n = 12), assigned as a negative control (NC) received only the vehicle, rats of the second set (n = 36), assigned as PCOS rats, were given letrozole 1mg/Kg orally for 21 days. On day 21, six rats from the first set and six rats from the second set were euthanized for confirmation of PCOS-induction. The remaining animals from the first set assigned as group 1, those in the second set (n = 30) were equally divided into 5 groups and treated daily for 19 days as follows: group 2 (positive control) received only the vehicle, group 3 treated with metformin 300mg/Kg orally, groups 4 and 5 treated respectively with 100 and 200 mg/Kg silibinin intraperitoneally (IP), and group 6 treated with a combination of metformin 300mg/Kg orally and silibinin 100mg/Kg IP. On day 40, blood samples were examined for luteinizing hormone (LH), testosterone (TS) and estradiol (EST) levels, the anti-inflammatory and antioxidant parameters, ovarian and uterine morphology. Results Silibinin alone or in combination with metformin was found to be effective in restoring the regularity of estrous cycle by ameliorating the abnormal alterations of LH, TS, EST, tumor necrosis factor (TNF)-α, and oxidative status and by resuming the appearance of corpora lutea and decreasing or even total absence of cystic follicles in the ovaries. Conclusion Silibinin was effective in restoring estrous regularities and alleviating hormonal and histomorphological abnormalities of the ovarian and uterine tissues, this could be due to its anti-androgenic, anti-inflammatory and antioxidant properties.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Marouf¹,

Ismaeel²,

Hassan³

et al. 2022

JIR

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“…Histomorphometric measurements confirmed that F. deltoidea increased the corpus luteum number and the endometrial thickness. These findings suggest the potential use of F. deltoidea as an adjuvant agent in the treatment program of PCOS [17].…”

Section: Resultsmentioning

confidence: 71%

“…Haslan M.A. and co-workers [17] investigated the biochemical, hormonal, and histomorphometric changes in letrozole (LTZ)-induced PCOS female rats following treatment with F. deltoidea. The results showed that treatment with F. deltoidea at the dose of 500 and 1000 mg/kg/day reduced insulin resistance, obesity indices, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL), malonic dialdehyde (MDA), testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) to nearnormal levels in PCOS rats.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Tkachenko¹,

Kurhaluk²,

Buyun³

et al. 2023

BHT

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Purpose: to investigate the antioxidant properties of the aqueous extracts derived from leaves of Ficus deltoidea using the model of human blood. For this purpose, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), content of aldehydic and ketonic derivatives of oxidatively modified proteins, total antioxidant capacity (TAC)] in the human erythrocytes after in vitro incubation with aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) were used. Resistance of human erythrocytes after in vitro treatment by aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) was evaluated by HCl-induced hemolysis using a percentage of hemolyzed erythrocytes per each 30 sec. and a total time of hemolysis. Methodology. The leaves of F. deltoidea were collected in M.M. Gryshko National Botanic Garden (Kyiv, Ukraine). Blood (10-20 mL) was obtained from normal volunteers via venipuncture (4 males and 5 females aged 28-53 years old). An erythrocyte suspension at 1% hematocrit was incubated with 4 mM phosphate buffer (pH 7.4) (control) and pre-incubated with the extract of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL, respectively) at 37 °C for 60 min. The level of lipid peroxidation was determined by quantifying the concentration of 2-thiobarbituric acid reacting substances (TBARS). The rate of protein oxidative destruction was estimated from the reaction of the resultant carbonyl derivatives of amino acid reaction with 2,4-dinitrophenylhydrazine (DNFH). The total antioxidant capacity (TAC) in the samples was estimated by measuring the TBARS level after Tween 80 oxidation. The HCl-induced resistance of erythrocytes was measured spectrophotometrically with 0.1M HCl. The significance of differences (significance level, p < 0.05) was examined using the Mann-Whitney U test. Scientific novelty. The treatment of human erythrocytes by extract derived from leaves of F. deltoidea at the final concentration of 5 mg/mL resulted in an increase of TBARS as biomarkers of lipid peroxidation and aldehydic derivatives of oxidatively modified proteins with a simultaneous decrease in the total antioxidant capacity compared to the untreated samples. On the other hand, in vitro treatment of human erythrocytes with an extract derived from leaves of F. deltoidea at the final concentration of 0.5 mg/mL resulted in the same values of TBARS level, aldehydic and ketonic derivatives of OMP, and total antioxidant capacity as the untreated samples. Extract of F. deltoidea at the final concentrations of 5 mg/mL and 0.5 mg/mL possess hemolytic properties to human erythrocyte suspension after 1-h incubation in vitro. Conclusions. The changes in the oxidative stress biomarkers using the in vitro model of human erythrocytes to evaluate the antioxidant activities of the aqueous extract derived from the leaves of F. deltoidea at the two final concentrations (5 mg/mL and 0.5 mg/mL) revealed that high concentration of extract (5 mg/mL) resulted in the increase of lipid peroxidation and protein oxidation with a simultaneous decline in the total antioxidant capacity. HCl-induced hemolysis was activated after the treatment by extract derived from the leaves of F. deltoidea at the two final concentrations. More studies are warranted in the future, to illustrate the potential and mechanisms of F. deltoidea in preventing oxidative stress using different cell models in vitro and different final concentrations of the extract. Also, further studies are warranted to identify the bioactive components that contribute to this protective effect.

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“…A total of 30 female mice (Mus musculus) at 3 months age, and weighing 30-40 grams (Lee's Index Value > 0.3) were divided randomly into three groups of 10, namely CTRL (n = 10, control without intervention), MISEM (n = 10, moderateintensity swimming exercise in the morning), and MISEN (n = 10, moderate-intensity swimming exercise in the night). Mice (Mus musculus) were placed in temperature-controlled steel cages (20-22 °C, 55-65% humidity) with a 12-h light/dark cycle (Haslan et al, 2021). This research has followed animal welfare principles published by the European Convention for the Protection of Vertebrate Animals.…”

Section: Experimental Designmentioning

confidence: 99%

Moderate-Intensity Swimming Exercises Decrease Body Weight and Lee’s Obesity Index in Female Mice (Mus musculus)

Antoni¹,

Rejeki²,

Sulistiawati³

et al. 2022

IJRP

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This study aims to analyze the effect of moderate-intensity swimming exercise which performed in the morning and at night on reducing body weight and Lee's obesity index in female mice (Mus musculus). This study used the true experiment method with the randomized control group post-test-only design. A total of 30 female mice (Mus musculus) at 3 months of age, and weighing 30 -40 grams (Lee's Obesity Index Value > 0.3) were divided randomly into three groups of 10, namely CTRL (n = 10, control without intervention), MISEM ( n = 10, moderate-intensity swimming exercise in the morning), and MISEN (n = 10, moderate-intensity swimming exercise at night). Body weight and Lee's obesity index was measured twice, namely preexercise and 4 weeks post-exercise. Then, data was analyzed using the Paired Samples T-Test with the Statistical Package for the Social Sciences SPSS version 21 software.The results show that the average body weight on CTRL between pre-exercise vs. post-exercise (33.60±2.17 vs. 39.20±3.82 gr (p-values=0.001)), )), and MISEN (33.40±3.47 vs. 26.00±2.26 gr (p-values=0.000)). Lee's average obesity index on CTRL between pre-exercise vs. post-exercise (0.39±0.02 vs. 0.44±0.02 gr/mm (p-values=0.000)), MISEM (0.40±0.02 vs. 0.42±0.01 gr/mm (p-values=0.000)), MISEN (0.41± 0.01 vs. 0.37±0.02 gr/mm (p-values=0.002)). Based on the results of the study, it was concluded that moderateintensity swimming exercise in the night was more effective in reducing body weight and Lee's obesity index than moderateintensity swimming exercise in the morning and control without intervention.

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Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats

Cited by 22 publications

References 59 publications

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Moderate-Intensity Swimming Exercises Decrease Body Weight and Lee’s Obesity Index in Female Mice (Mus musculus)

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