Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei

Liang, QingJian; Dong, Wenna; Wang, Feifei; Wang, Weina; Zhang, Jian; Liu, Xing

doi:10.1016/j.ecoenv.2021.112989

Cited by 7 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The suppression of WSSV infection may have been caused by physiological disorders (e.g., increase in oxidative stress and damage to tissue integrity) in shrimps at a relatively high concentration of ammonia in the culture water. To clarify whether ammonia stress damages the host physiology, we measured the total antioxidant capacity, catalase activity, superoxide dismutase activity, and glutathione peroxidase activity in the hepatopancreas, which are important indicators of host physiology ( 26 , 27 ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ammonia stress-induced heat shock factor 1 enhances white spot syndrome virus infection by targeting the interferon-like system in shrimp

Wang,

Zhang,

Gao

et al. 2024

mBio

View full text Add to dashboard Cite

Disease emergence is the consequence of host-pathogen-environment interactions. Ammonia is a key stress factor in aquatic environments that usually increases the risk of pathogenic diseases in aquatic animals. However, the molecular regulatory mechanisms underlying the enhancement of viral infection following ammonia stress remain largely unknown. Here, we found that ammonia stress enhances white spot syndrome virus infection in kuruma shrimp ( Marsupenaeus japonicus ) by targeting the antiviral interferon-like system through heat shock factor 1 (Hsf1). Hsf1 is an ammonia-induced transcription factor. It regulates the expression of Cactus and Socs2 , which encode negative regulators of NF-κB signaling and Jak/Stat signaling, respectively. By inhibiting these two pathways, ammonia-induced Hsf1 suppressed the production and function of MjVago-L, an arthropod interferon analog. Therefore, this study revealed that Hsf1 is a central regulator of suppressed antiviral immunity after ammonia stress and provides new insights into the molecular regulation of immunity in stressful environments. IMPORTANCE Ammonia is the end product of protein catabolism and is derived from feces and unconsumed foods. It threatens the health and growth of aquatic animals. In this study, we demonstrated that ammonia stress suppresses shrimp antiviral immunity by targeting the shrimp interferon-like system and that heat shock factor 1 (Hsf1) is a central regulator of this process. When shrimp are stressed by ammonia, they activate Hsf1 for stress relief and well-being. Hsf1 upregulates the expression of negative regulators that inhibit the production and function of interferon analogs in shrimp, thereby enhancing white spot syndrome viral infection. Therefore, this study, from a molecular perspective, explains the problem in the aquaculture industry that animals living in stressed environments are more susceptible to pathogens than those living in unstressed conditions. Moreover, this study provides new insights into the side effects of heat shock responses and highlights the complexity of achieving cellular homeostasis under stressful conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Ammonia stress-induced heat shock factor 1 enhances white spot syndrome virus infection by targeting the interferon-like system in shrimp

Wang,

Zhang,

Gao

et al. 2024

mBio

View full text Add to dashboard Cite

show abstract

“…5 Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangzhou 510515, China. 6 Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology, Guangzhou 510515, China.…”

Section: Availability Of Data and Materialsmentioning

confidence: 99%

“…In south China, especially for Guangdong, Guangxi and Hainan provinces, the dry root (Radix Fici Hirtae) of this species has been used as an ethnic herbal medicine with the effects of dispelling the dampness and invigorating the spleen, nourishing the lung for a long time in Yao and Zhuang National Regions [ 2 – 4 ]. It contains many active compounds such as coumarins, volatile oils, amino acids, saccharides, steroids, phenolics, flavonoids and phenylpropanoids along with multiple therapeutic effects such as antitumor, antifungal and hepatoprotective which were confirmed by modern pharmacological researches [ 5 , 6 ]. In addition to the medicinal values, it is also an edible healthy food with special aroma that has been widely used in food industry.…”

Section: Introductionmentioning

confidence: 99%

High genetic diversity and low population differentiation of a medical plant Ficus hirta Vahl., uncovered by microsatellite loci: implications for conservation and breeding

Lü

Chen

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Wuzhimaotao (Radix Fici Hirtae) originates from the dry root of Ficushirta (Moraceae), which is widely known as a medical and edible plant distributed in South China. As the increasing demand for Wuzhimaotao, the wild F.hirta has been extremely reduced during the past years. It is urgent to protect and rationally develop the wild resources of F.hirta for its sustainable utilization. However, a lack of genetic background of F.hirta makes it difficult to plan conservation and breeding strategies for this medical plant. In the present study, a total of 414 accessions of F.hirta from 7 provinces in southern China were evaluated for the population genetics using 9 polymorphic SSR markers. Results A mean of 17.1 alleles per locus was observed. The expected heterozygosity (He) varied from 0.142 to 0.861 (mean = 0.706) in nine SSR loci. High genetic diversity (He = 0.706, ranged from 0.613 to 0.755) and low genetic differentiation among populations (G’ST = 0.147) were revealed at population level. In addition, analysis of molecular variance (AMOVA) indicated that the principal molecular variance existed within populations (96.2%) was significantly higher than that among populations (3.8%). Meanwhile, the three kinds of clustering methods analysis (STRUCTURE, PCoA and UPGMA) suggested that the sampled populations were clustered into two main genetic groups (K = 2). Mantel test showed a significant correlation between geographic and genetic distance among populations (R2 = 0.281, P < 0.001). Pollen flow, seed flow and/or geographical barriers might be the main factors that formed the current genetic patterns of F.hirta populations. Conclusions This is a comprehensive study of genetic diversity and population structure of F.hirta in southern China. We revealed the high genetic diversity and low population differentiation in this medicinal plant and clarified the causes of its current genetic patterns. Our study will provide novel insights into the exploitation and conservation strategies for F.hirta.

show abstract

Physiology of medicinal and aromatic plants under drought stress

Emami Bistgani,

Barker,

Hashemi

2024

The Crop Journal

View full text Add to dashboard Cite

Ficus hirta Vahl. promotes antioxidant enzyme activity under ammonia stress by inhibiting miR-2765 expression in Penaeus vannamei

Cited by 7 publications

References 37 publications

Ammonia stress-induced heat shock factor 1 enhances white spot syndrome virus infection by targeting the interferon-like system in shrimp

Ammonia stress-induced heat shock factor 1 enhances white spot syndrome virus infection by targeting the interferon-like system in shrimp

High genetic diversity and low population differentiation of a medical plant Ficus hirta Vahl., uncovered by microsatellite loci: implications for conservation and breeding

Physiology of medicinal and aromatic plants under drought stress

Contact Info

Product

Resources

About