Abstract:During CRISPR adaptation, short sections of invader derived DNA of defined length are specifically integrated at the leader-repeat junction as spacers by Cas1-2 integrase complex. While several variants of CRISPR systems utilise Cas4 as an indispensible nuclease for processing the PAM containing prespacers to a defined length for integration-surprisingly-a few CRISPR systems such as type I-E are bereft of Cas4.Therefore, how the prespacers show impeccable conservation for length and PAM selection in type I-E r… Show more
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