“…However, in conventional detection, two separate steps are required, potentially resulting in the generation of enduring amplicon aerosol contaminants, which can lead to false-positive results. To address this issue, a solution is proposed in the form of a one-tube assay that employs CRISPR-Cas13a in conjunction with reverse transcription-recombinase polymerase amplification, effectively preventing the occurrence of aerosol pollution . The one-tube assay can be accomplished within a brief span of 40 min, yielding a sensitivity capable of detecting as few as 180 RNA copies per reaction.…”