2023
DOI: 10.1016/j.jhazmat.2023.132077
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Field-deployable assay based on CRISPR-Cas13a coupled with RT-RPA in one tube for the detection of SARS-CoV-2 in wastewater

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Cited by 8 publications
(1 citation statement)
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“…However, in conventional detection, two separate steps are required, potentially resulting in the generation of enduring amplicon aerosol contaminants, which can lead to false-positive results. To address this issue, a solution is proposed in the form of a one-tube assay that employs CRISPR-Cas13a in conjunction with reverse transcription-recombinase polymerase amplification, effectively preventing the occurrence of aerosol pollution . The one-tube assay can be accomplished within a brief span of 40 min, yielding a sensitivity capable of detecting as few as 180 RNA copies per reaction.…”
Section: Detection Of Biomarkers In (Waste)water and (Bio)environmentmentioning
confidence: 99%
“…However, in conventional detection, two separate steps are required, potentially resulting in the generation of enduring amplicon aerosol contaminants, which can lead to false-positive results. To address this issue, a solution is proposed in the form of a one-tube assay that employs CRISPR-Cas13a in conjunction with reverse transcription-recombinase polymerase amplification, effectively preventing the occurrence of aerosol pollution . The one-tube assay can be accomplished within a brief span of 40 min, yielding a sensitivity capable of detecting as few as 180 RNA copies per reaction.…”
Section: Detection Of Biomarkers In (Waste)water and (Bio)environmentmentioning
confidence: 99%