Field-effect biosensor using virus particles as scaffolds for enzyme immobilization

Poghossian, Arshak; Jablonski, Melanie; Koch, Claudia; Bronder, Thomas; Rolka, David; Wege, Christina; Schöning, Michael J.

doi:10.1016/j.bios.2018.03.036

Cited by 54 publications

(63 citation statements)

References 56 publications

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“…More recently, a novel promising approach for the development of EnEIS biosensors was described in [ 103 , 114 ], where a highly sensitive penicillin biosensor with a superior lifetime was realized by means of modification of a Ta 2 O 5 -gate EIS structure with tobacco mosaic virus (TMV) particles as scaffolds for the dense immobilization of enzymes. The TMV has a nanotube-like structure with an average length of 300 nm, an outer diameter of 18 nm, and an internal channel of 4 nm in diameter [ 115 , 116 ].…”

Section: Chemical Sensors and Biosensors Based On Capacitive Eis Smentioning

confidence: 99%

“…Most likely, this novel approach may be adapted to other enzymes. The results obtained in [ 103 ] demonstrate a great potential for the integration of plant virus/receptor nanohybrids with electronic chips, thereby opening new opportunities in advanced biosensing technologies.…”

Section: Chemical Sensors and Biosensors Based On Capacitive Eis Smentioning

confidence: 99%

“…Although, during the last years, a number of technological solutions have been proposed and tested to solve these problems and to improve the working parameters of EnEIS biosensors, their transfer from research laboratories to real-life applications remains rather slow. Some examples for practical applications include EnEIS biosensors for the detection of glucose [ 81 , 82 , 87 ], creatinine [ 81 , 82 ], urea [ 82 ], and total triglyceride level [ 66 ] in human serum, glucose level in whole blood [ 88 ], acetoin in diluted white wine samples [ 80 ], and penicillin in bovine milk [ 103 ].…”

Section: Chemical Sensors and Biosensors Based On Capacitive Eis Smentioning

confidence: 99%

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Capacitive Field-Effect EIS Chemical Sensors and Biosensors: A Status Report

Poghossian¹,

Schöning

2020

Sensors

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Electrolyte-insulator-semiconductor (EIS) field-effect sensors belong to a new generation of electronic chips for biochemical sensing, enabling a direct electronic readout. The review gives an overview on recent advances and current trends in the research and development of chemical sensors and biosensors based on the capacitive field-effect EIS structure—the simplest field-effect device, which represents a biochemically sensitive capacitor. Fundamental concepts, physicochemical phenomena underlying the transduction mechanism and application of capacitive EIS sensors for the detection of pH, ion concentrations, and enzymatic reactions, as well as the label-free detection of charged molecules (nucleic acids, proteins, and polyelectrolytes) and nanoparticles, are presented and discussed.

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Section: Chemical Sensors and Biosensors Based On Capacitive Eis Smentioning

confidence: 99%

Section: Chemical Sensors and Biosensors Based On Capacitive Eis Smentioning

confidence: 99%

Section: Chemical Sensors and Biosensors Based On Capacitive Eis Smentioning

confidence: 99%

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Capacitive Field-Effect EIS Chemical Sensors and Biosensors: A Status Report

Poghossian¹,

Schöning

2020

Sensors

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“…These include an appropriate robustness despite biodegradability, and sustainable production routes in cell cultures and, to an increasing extent, plant‐based expression systems (Bally et al, ; Buyel, ; Gleba, Klimyuk, & Marillonnet, ; Lomonossoff & D'Aoust, ; Marillonnet, Thoeringer, Kandzia, Klimyuk, & Gleba, ; Marsian & Lomonossoff, ; Rybicki, ; Sack, Hofbauer, Fischer, & Stoger, ; J. Xu, Towler, & Weathers, ). Of primary importance are their repetitive interior and exterior protein surfaces with selectively addressable coupling sites shown to stabilize biomolecules in a striking manner (Poghossian et al, ). Most significant for this review, however, is an amenability of particular plant viral components to self‐assemble into a large variety of unnatural, but useful and technically not accessible nanoarchitectures.…”

Section: Conclusion Costs Perspectivesmentioning

confidence: 99%

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Wege

Koch

2019

WIREs Nanomed Nanobiotechnol

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The self‐assembly of viral building blocks bears exciting prospects for fabricating new types of bionanoparticles with multivalent protein shells. These enable a spatially controlled immobilization of functionalities at highest surface densities—an increasing demand worldwide for applications from vaccination to tissue engineering, biocatalysis, and sensing. Certain plant viruses hold particular promise because they are sustainably available, biodegradable, nonpathogenic for mammals, and amenable to in vitro self‐organization of virus‐like particles. This offers great opportunities for their redesign into novel “green” carrier systems by spatial and structural synthetic biology approaches, as worked out here for the robust nanotubular tobacco mosaic virus (TMV) as prime example. Natural TMV of 300 x 18 nm is built from more than 2,100 identical coat proteins (CPs) helically arranged around a 6,395 nucleotides ssRNA. In vitro, TMV‐like particles (TLPs) may self‐assemble also from modified CPs and RNAs if the latter contain an Origin of Assembly structure, which initiates a bidirectional encapsidation. By way of tailored RNA, the process can be reprogrammed to yield uncommon shapes such as branched nanoobjects. The nonsymmetric mechanism also proceeds on 3'‐terminally immobilized RNA and can integrate distinct CP types in blends or serially. Other emerging plant virus‐deduced systems include the usually isometric cowpea chlorotic mottle virus (CCMV) with further strikingly altered structures up to “cherrybombs” with protruding nucleic acids. Cartoon strips and pictorial descriptions of major RNA‐based strategies induct the reader into a rare field of nanoconstruction that can give rise to utile soft‐matter architectures for complex tasks. This article is categorized under: Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures

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“…2). The surface of TMV has a strong stabilizing effect on different enzymes, including glucose oxidase, horseradish peroxidase (Baecker et al, 2017;Koch et al, 2015), and penicillinase Poghossian et al, 2018). The use of TMV adapter rods on sensor surfaces (see below) also has enabled bioaffinity-based display of streptavidin conjugates of these enzymes at surface densities not achievable on TMV-free supports, with the additional advantages of increased reusability and enhanced target detection ranges of such devices.…”

Section: Display Of Active Enzymesmentioning

confidence: 99%

TMV Particles: The Journey From Fundamental Studies to Bionanotechnology Applications

Lomonossoff

Wege

2018

Advances in Virus Research

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Ever since its initial characterization in the 19th century, tobacco mosaic virus (TMV) has played a prominent role in the development of modern virology and molecular biology. In particular, research on the three-dimensional structure of the virus particles and the mechanism by which these assemble from their constituent protein and RNA components has made TMV a paradigm for our current view of the morphogenesis of self-assembling structures, including viral particles. More recently, this knowledge has been applied to the development of novel reagents and structures for applications in biomedicine and bionanotechnology. In this article, we review how fundamental science has led to TMV being at the vanguard of these new technologies.

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Field-effect biosensor using virus particles as scaffolds for enzyme immobilization

Cited by 54 publications

References 56 publications

Capacitive Field-Effect EIS Chemical Sensors and Biosensors: A Status Report

Capacitive Field-Effect EIS Chemical Sensors and Biosensors: A Status Report

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

TMV Particles: The Journey From Fundamental Studies to Bionanotechnology Applications

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