Field evaluation of dual inoculation of alfalfa with <i>Sinorhizobium meliloti</i> and <i>Penicillium bilaii</i>

Rice, W. A.; Lupwayi, Newton Z.; Olsen, P. E.; Schlechte, D.; Gleddie, S. C.

doi:10.4141/p99-055

Cited by 12 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P. bilaiae does not produce any known toxic secondary metabolites (Savard et al 1994) and stimulates production of root hairs (Gulden and Vessey 2000) and an increase in overall root growth (Vessey and Heisinger 2001). Inoculation with P. bilaiae has improved growth of many crop species including wheat (Kucey 1987;Kucey 1988;Anstis 2004), canola and grain and pasture legumes (Beckie et al 1998;Rice et al 2000). A commercial product based on P. bilaiae has been used in North America for at least 15 years and was released in Australia in 2010.…”

Section: Microbial Inoculantsmentioning

confidence: 97%

See 1 more Smart Citation

Plant and microbial strategies to improve the phosphorus efficiency of agriculture

et al. 2011

View full text Add to dashboard Cite

Background Agricultural production is often limited by low phosphorus (P) availability. In developing countries, which have limited access to P fertiliser, there is a need to develop plants that are more efficient at low soil P. In fertilised and intensive systems, P-efficient plants are required to minimise inefficient use of P-inputs and to reduce potential for loss of P to the environment. Scope Three strategies by which plants and microorganisms may improve P-use efficiency are outlined: (i) Root-foraging strategies that improve P acquisition by lowering the critical P requirement of plant growth and allowing agriculture to operate at lower levels of soil P; (ii) P-mining strategies to enhance the desorption, solubilisation or mineralisation of P from sparingly-available sources in soil using root exudates (organic anions, phosphatases), and (iii) improving internal P-utilisation efficiency through the use of plants that yield more per unit of P uptake.Conclusions We critically review evidence that more P-efficient plants can be developed by modifying root growth and architecture, through manipulation of root exudates or by managing plant-microbial associations such as arbuscular mycorrhizal fungi and microbial inoculants. Opportunities to develop P-efficient plants through breeding or genetic modification are described and issues that may limit success including potential trade-offs and trait interactions are discussed. Whilst demonstrable progress has been made by selecting plants for root morphological traits, the potential for manipulating root physiological traits or selecting plants for low internal P concentration has yet to be realised.

show abstract

Section: Microbial Inoculantsmentioning

confidence: 97%

“…and AM fungi, and for legumes with Penicillium and Rhizobium spp. (Downey and van Kessel 1990;Rice et al 2000), with the later also being released as a commercial inoculant.…”

Section: Microbial Inoculantsmentioning

confidence: 99%

Plant and microbial strategies to improve the phosphorus efficiency of agriculture

et al. 2011

View full text Add to dashboard Cite

show abstract

“…P. bilaiae has also been reported to stimulate production of root hairs (Gulden and Vessey 2000) and increase overall root growth (Vessey and Heisinger 2001). Inoculation with P. bilaiae has improved growth of many cropping species including wheat (Kucey 1987(Kucey , 1988Anstis 2004), canola , and grain (Kucey 1987;Gleddie 1993) and pasture (Beckie et al 1998;Rice et al 2000) legumes. A commercial inoculant product based on P. bilaiae (JumpStartÔ), has been successfully used by Canadian wheat growers to improve crop P nutrition for at least 15 years.…”

Section: Penicillium-based Inoculants To Improve P Acquisition and Plmentioning

confidence: 99%

“…maintain rhizoshere potential via profuse sporulation). The most effective isolate was identified as a novel strain of P. bilaiae (Wakelin et al 2004b), the same species that is being used successfully in North America to promote higher P-fertiliser efficiency and increase plant production Beckie et al 1998;Rice et al 2000). The Australian strain of P. bilaiae significantly increased carbonate-extractable P in soil (pH 7.3) by 23% compared with the uninoculated control, improving P uptake in lentils in three neutral to alkaline cropping soils (Wakelin et al 2007b).…”

Section: Penicillium-based Inoculants To Improve P Acquisition and Plmentioning

confidence: 99%

Potential to improve root access to phosphorus: the role of non-symbiotic microbial inoculants in the rhizosphere

2009

View full text Add to dashboard Cite

Phosphate anions in soil solution are extremely reactive and may be rapidly immobilised in the soil through precipitation and adsorption reactions, resulting in sparingly soluble forms of phosphorus (P) that are essentially unavailable to plants. This low P-fertiliser efficiency is often offset through high application rates, which are economically and environmentally unsustainable and not an available option for organic producers. Microorganisms play a fundamental role in the biogeochemical cycling of inorganic and organic P in the rhizosphere and detritusphere. Free-living rhizosphere microbes can directly increase the availability of phosphate to plant roots via mechanisms associated with solubilisation and mineralisation of P from inorganic and organic forms of total soil P. These include releasing organic anions, H + ions, phosphatases, and cation chelating compounds into the rhizosphere. Many soil-borne microbes also increase P availability indirectly by producing phytohormones that increase root density and function. There is increasing interest worldwide in the use of rhizosphere microorganisms as inoculants to increase P availability for agricultural production. Recent research has focussed on developing actively sporulating Penicillium fungi known to express mechanisms to enhance P mobilisation and therefore, considered to be a key component of the mycoflora involved in P cycling in soils. Penicillium species do not exhibit specific plant or soil associations and have a broad agro-ecological range, indicating their potential to be developed as inoculants for a range of plant production systems. Successful adoption of microbial inoculants requires a thorough understanding of their rhizosphere ecology, genetic stability, and the mechanisms associated with enhancing P availability in soils and plant-growth promotion. This will provide a better understanding of which inoculants to use under particular agro-ecological conditions for increased efficacy and consistent performance.

show abstract

“…These experiments typically involved the use of 10 5 to 10 6 bacteria per seed or per g of soil to inoculate soils containing indigenous S. meliloti populations of up to 10 3 bacteria per g of soil (3,17,19,21,27,28). (ii) Persistence.…”

Section: Resultsmentioning

confidence: 99%

Construction and Environmental Release of a Sinorhizobium meliloti Strain Genetically Modified To Be More Competitive for Alfalfa Nodulation

Dillewijn

Soto

Villadas

et al. 2001

Appl Environ Microbiol

View full text Add to dashboard Cite

؊ mutant strain, that proline dehydrogenase activity is required for colonization and therefore for the nodulation efficiency and competitiveness of S. meliloti on alfalfa roots (J. I. Jiménez-Zurdo, P. van Dillewijn, M. J. Soto, M. R. de Felipe, J. Olivares, and N. Toro, Mol. Plant-Microbe Interact. 8:492-498, 1995). In this work, we investigated whether the putA gene could be used as a means of increasing the competitiveness of S. meliloti strains. We produced a construct in which a constitutive promoter was placed 190 nucleotides upstream from the start codon of the putA gene. This resulted in an increase in the basal expression of this gene, with this increase being even greater in the presence of the substrate proline. We found that the presence of multicopy plasmids containing this putA gene construct increased the competitiveness of S. meliloti in microcosm experiments in nonsterile soil planted with alfalfa plants subjected to drought stress only during the first month. We investigated whether this construct also increased the competitiveness of S. meliloti strains under agricultural conditions by using it as the inoculum in a contained field experiment at León, Spain. We found that the frequency of nodule occupancy was higher with inoculum containing the modified putA gene for samples that were analyzed after 34 days but not for samples that were analyzed later.

show abstract

Field evaluation of dual inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaii

Cited by 12 publications

References 10 publications

Plant and microbial strategies to improve the phosphorus efficiency of agriculture

Plant and microbial strategies to improve the phosphorus efficiency of agriculture

Potential to improve root access to phosphorus: the role of non-symbiotic microbial inoculants in the rhizosphere

Construction and Environmental Release of a Sinorhizobium meliloti Strain Genetically Modified To Be More Competitive for Alfalfa Nodulation

Contact Info

Product

Resources

About