Field evaluation of hazardous waste site bioassessment protocols

Thomas, J.M.; Cline, J.F.; Cushing, Colbert E.; McShane, M.C.; Rogers, John E.; Rogers, L.E.; Simpson, Josh; Skalski, John R.

doi:10.2172/6193843

Cited by 4 publications

(4 citation statements)

References 2 publications

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“…Soil samples were collected across a waste ditch leading to the active holding basin using a standard shovel. Additional details and maps are described elsewhere [4].…”

Section: Study Sitementioning

confidence: 99%

Modification of the Neubauer Technique to Assess Toxicity of Hazardous Chemicals in Soils

Thomas¹,

Cline²

1985

Environ Toxicol Chem

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The Neubauer technique was modified to provide a sensitive and economical phytoassay for soils and surface waters obtained from a chemical waste site. Use of individual plastic enclosures allowed safe handling and disposal over the course of our experiments. Laboratory tests showed that water from a holding basin was toxic to wheat plants at dilutions of less than 1% and that our modified Neubauer technique produced results compatible with both pot culture and the standard Neubauer test. Further testing of several inorganic constituents of the basin water pointed to an organic toxicant, even though the original water contained high levels of sodium, copper and other elements.The results of testing 26 samples from an abandoned waste pond were negative insofar as toxicity to wheat and lettuce seeds, whereas samples from an abandoned ditch allowed us to determine areal toxicity as well as toxicity as a function of depth and suggested that more than one species should be tested.

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“…Soil samples were collected across a waste ditch leading to the active holding basin using a standard shovel. Additional details and maps are described elsewhere [4].…”

Section: Study Sitementioning

confidence: 99%

Modification of the Neubauer Technique to Assess Toxicity of Hazardous Chemicals in Soils

Thomas¹,

Cline²

1985

Environ Toxicol Chem

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“…During the week of 1 July 1983 the colonies \vere taken to Denver, Colorado. Treatment sites were established on the RMA with eight colonies each near F-Basin and Derby Lakes (see Thomas et al 1983 for complete site description). A control site with eight colonies was located approximately 35 miles northwest of RMA near Lyons, Colorado.…”

Section: T•1ethodsmentioning

confidence: 99%

“…During the preliminary study on the Rocky Mountain Arsenal (R~lA) in 1982, several invertebrate groups were evaluated for potential use as indicators of the presence of toxic materials in the environment. The most premising was the use of honeybees and the determination of their brood survival (Thomas et al 1983).…”

Section: Introductionmentioning

confidence: 99%

Field evaluation of hazardous waste site bioassessment protocols. Volume 2

Thomas¹,

Cline²,

Gano³

et al. 1984

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“…Terrestrial biaassays that ~ave been used include crickets (V.la:ton 1980; Burkhardt ar.d Fa~rchi1d :967), ear:. :hworrns, so~1 respiration, ar.d sclerotia formation by a species of the fungus .A.soeroillus (Thomas et al 1983). In this study, harvester ar~ts were investigated as an additional terrestrial organism for bioassay.…”

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confidence: 99%

Harvester ant bioassay for assessing hazardous chemical waste sites. [Pogonomyrmex owhyeei]

Gano¹,

Carlile²,

Rogers³

1985

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A technique was developed for using harvester ants, Pogonomyrmex owhyeei, in terrestrial bioassays. Procedures \'/ere developed for maintaining stock populations, handling ants, and exposing ants to toxic materials. Relative toxicities were determined by exposing ants to 10 different materials. These materials included three insecticides, Endrin, Aldrin, and Dieldrin; one herbicide, 2,4-D; three complex industrial waste residuals, wood preservative sludge, drilling fluid, and slop oil; and three heavy metals, copper zinc, and cadium. Ants were exposed in petri dishes containing soil amended with a particular toxicant. Under these test conditions, ants showed no sensitivity to the metals or 2,4-D. Ants were sensitive to the insecticides and oils in repeated tests, and relative toxicity remained consistent throughout. Aldrin was the most toxic material followed by Dieldrin, Endrin, wood preservative sludge, drilling fluid, and slop oil .

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Field evaluation of hazardous waste site bioassessment protocols

Cited by 4 publications

References 2 publications

Modification of the Neubauer Technique to Assess Toxicity of Hazardous Chemicals in Soils

Modification of the Neubauer Technique to Assess Toxicity of Hazardous Chemicals in Soils

Field evaluation of hazardous waste site bioassessment protocols. Volume 2

Harvester ant bioassay for assessing hazardous chemical waste sites. [Pogonomyrmex owhyeei]

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