Field evaluation of sex pheromones and binding specificity of pheromone binding protein 4 in Tryporyza intacta (Lepidoptera: Crambidae)

Abstract

The recognition of chemical signal including volatile odorants and pheromones is very important in the olfactory physiological behaviors of insects, such as avoiding predators, seeking food and mating partners. The sugarcane borer, Tryporyza intacta is the most harmful insect in sugarcane region in Southeast Asia and Southern China, however, the study of their molecular biology and physiology was limited. Here we demonstrated that the sex pheromone (E11-16:Ald: Z11-16:Ald = 7:3) were most effective to T. intacta. In addition, compared the traditional rubber lure, a new microsphere formulation lure can optimize the trapping effect and might be widely used in the sugarcane growing area. To obtain a better understanding of the olfactory molecular mechanism of pheromone-based mate recognition system, we have cloned the full-length gene of the TintPBP4 and expressed in Escherichia coli. Our phylogenetic analysis highlighted that the TintPBP4 was highly conserved among diverse species of Lepidoptera. Furthermore, the results of QRT-PCR demonstrated that TintPBP4 transcripts were abundantly expressed in the antennae of T. intacta, especially in the male adults. The fluorescence binding experiments showed the TintPBP4 exhibited strong binding capacities to the sex pheromone components. These results will not only provide more understanding for the functional analysis of olfactory proteins from T. intacta, but also assist in the exploitation and development of sex pheromones in the integrated biological control of this pest.

Molecular Characterization and Expression Patterns of Two Pheromone-Binding Proteins from the Diurnal Moth Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae)

“…Only two PflaPBPs were involved in this study, while 3~4 PBPs have been identified in some lepidopteran species [74,75]. These two PflaPBPs were originally identified from an antenna Illumina high-throughput transcriptome (unpublished data) in P. flammans, and their binding affinity for Z-9-hexadecenal and (Z, Z, Z)-9, 12, 15-octadecatrienal was also confirmed.…”

Metabolite profiling and in-silico studies show multiple effects of insecticidal actinobacterium on Spodoptera littoralis

“…The crude constituents of ES2 combine a variety of biological activities, including biocontrol, protein inhibitor, neurotoxin, antioxidant, immunostimulant, and anticancer activities. The compound 1-naphthylamine has antitumor 41 , antimicrobial 42 , and biocontrol activities against a lepidopteran pest, Tryporyza intacta 43 . S-adenosylmethioninaminium has anticancer activity 44 , whereas Brevianamid F and Palitantin have antimicrobial activity 45 , 46 .…”

Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata (Lepidoptera: Crambidae)

“…These results indicated that hydrogen bonds and hydrophobic cavity may play important roles in the olfactory recognition process of OBP3 protein to host-plant volatiles. A great number of studies reported that different subfamilies of insect OBPs were involved in recognition and transport of semiochemical components and olfactory signals through the hemolymph in chemosensory organs [9][10][11][12][13][14] . For instance, Li et al found that Eastern honey bee, Apis cerana OBP2 had two key amino acids, Ser123 and Lys51 by docking analysis and site-directedmutagenesis, exhibiting important olfactory physiological function in visiting ower and pollination of Eastern honey bee 12 .…”

“…Generally, OBPs can carry lipophilic volatiles of host and prey to the olfactory receptor (Ors) cells through hemolymph circulation of antennae and function in the rst step of insect odor reception 9 . PBPs are mainly involved in the chemical communication of sex pheromones between male and female pests and bene cial insects, which are produced in the pheromone glands (PG) located on the abdomen terminals 10 .…”