Field Evaluation of Transgenic Tobacco Containing Genes Encoding Bacillus thuringiensis ∂-Endotoxin or Cowpea Trypsin Inhibitor: Efficacy Against Helicoverpa zea (Lepidoptera: Noctuidae)

Hoffmann, Michael P.; Zalom, Frank G.; Wilson, L. T.; Smilanick, J. M.; Malyj, Lorraine D.; Kiser, John; Hilder, Vaughan A.; Barnes, Wayne M.

doi:10.1093/jee/85.6.2516

Cited by 91 publications

(25 citation statements)

References 11 publications

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“…Transgenic expressing Cowpea Trypsin Inhibitor gene have shown promising effect against tobacco budworm (Heliothis virescens) leads to increased mortality, reduced growth, and reduced plant damage (Hilder et al 1987). Similar results were obtained against corn earworm (Helicoverpa zea) in US (Hoffmann et al 1992). Transgenic study of soybean Kunitz trypsin inhibitors (TIs) in tobacco have show promising results against H. virescence (Lepidoptera) (Gatehouse et al 1993).…”

Section: Introductionmentioning

confidence: 81%

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Ghodke

Chavan

Sonawane

et al. 2013

Journal of Plant Interactions

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Section: Introductionmentioning

confidence: 81%

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Ghodke

Chavan

Sonawane

et al. 2013

Journal of Plant Interactions

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“…Bt-transgenic tobacco was compared to cowpea trypsin inhibitor-transgenic tobacco in field trials (Hoffmann et al, 1992). Each transgenic was protected from damage by the corn earworm, Helicoverpa zea.…”

Section: Transgenic Plants With Bt Toxin and Protease Inhibitor Genesmentioning

confidence: 99%

Protease interactions with Bacillus thuringiensis insecticidal toxins

Oppert

1999

Arch Insect Biochem Physiol

111

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“…The majority of plant transformations using Bt have been performed with native genes (Barton et al, 1987;Vaeck et al, 1987;Perlak et al, 1990;Mehra-Palta et al, 1991;Murray et al, 1991;Hoffmann et al, 1992). Since plants do not have many of the tRNAs required by the bacterial gene codons, transgenic plants often express these native genes at low levels and do not kill insects that feed on them (Adang et al, 1993).…”

mentioning

confidence: 99%

Genetic Transformation, Recovery, and Characterization of Fertile Soybean Transgenic for a Synthetic Bacillus thuringiensis cryIAc Gene

et al. 1996

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Somatic embryos of Jack, a Glycine max (1.) Merrill cultivar, were transformed using microprojectile bombardment with a synthetic Bacillus thuringiensis insecticidal crystal protein gene (Bt crylAc) driven by the 35s promoter and linked t o the HPH gene.Approximately 10 g of tissue was bombarded, and three transgenic lines were selected on hygromycin-containing media and converted into plants. The recovered lines contained the HPHgene, but the Bt gene was lost in one line. The plasmid was rearranged in the second line, and the third line had two copies, one of which was rearranged. The CrylAc protein accumulated up to 46 ng mg-' extractable protein. I n detached-leaf bioassays, plants with an intact copy of the Bt gene, and t o a lesser extent those with the rearranged copy, were protected from damage from corn earworm (Helicoverpa zea), soybean looper (Pseudoplusia includens), tobacco budworm (Heliothis virescens), and velvetbean caterpillar (Anticarsia gemmatalis). Corn earworm produced less than 3% defoliation on transgenic plants, compared with 20% on the lepidopteran-resistant breeding line CatlR81-296, and more than 40% on susceptible cultivars. Unlike previous reports of soybean transformation using this technique, all plants were fertile. To our knowledge, this is the first report of a soybean transgenic for a highly expressed insecticidal gene.

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Field Evaluation of Transgenic Tobacco Containing Genes Encoding Bacillus thuringiensis ∂-Endotoxin or Cowpea Trypsin Inhibitor: Efficacy Against Helicoverpa zea (Lepidoptera: Noctuidae)

Cited by 91 publications

References 11 publications

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Protease interactions with Bacillus thuringiensis insecticidal toxins

Genetic Transformation, Recovery, and Characterization of Fertile Soybean Transgenic for a Synthetic Bacillus thuringiensis cryIAc Gene

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