Field Evaluation on Growth and Productivity of the Transgenic Sugarcane Lines Overexpressing Sucrose-Phosphate Synthase

Suherman,; Wijayanto, Satrio Ilham; Anur, Risky Mulana; Neliana, Intan Ria; Dewanti, Parawita; Sugiharto, Bambang

doi:10.1007/s12355-022-01121-7

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Protein and potassium content was higher in stems of transgenic lines. However, wild-type and transgenic sugarcane were found to be substantially equivalent in terms of nutritional and mineral compositions [126]. The development of new sucrose isomers, trehalulose and isomaltulose, is facilitated by the insertion of sucrose isomerase genes.…”

Section: Production Of New Compounds and Renewable Energy Sourcesmentioning

confidence: 99%

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Kumar,

Wang,

et al. 2024

Plants

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Sugarcane, a vital cash crop, contributes significantly to the world’s sugar supply and raw materials for biofuel production, playing a significant role in the global sugar industry. However, sustainable productivity is severely hampered by biotic and abiotic stressors. Genetic engineering has been used to transfer useful genes into sugarcane plants to improve desirable traits and has emerged as a basic and applied research method to maintain growth and productivity under different adverse environmental conditions. However, the use of transgenic approaches remains contentious and requires rigorous experimental methods to address biosafety challenges. Clustered regularly interspaced short palindromic repeat (CRISPR) mediated genome editing technology is growing rapidly and may revolutionize sugarcane production. This review aims to explore innovative genetic engineering techniques and their successful application in developing sugarcane cultivars with enhanced resistance to biotic and abiotic stresses to produce superior sugarcane cultivars.

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Section: Production Of New Compounds and Renewable Energy Sourcesmentioning

confidence: 99%

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Kumar,

Wang,

et al. 2024

Plants

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Enhancing sugarcane growth quality and productivity through a biotechnology approach

Sugiharto

2024

BIO Web Conf.

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Sugarcane is a major crop to produce sugar accounting for nearly 80% of sugar production worldwide. Sugarcane is well adapted to warm climates and accumulated high biomass quantities for bioelectricity and second-generation bioethanol. Although Indonesia is one among the cane sugar producers, the produced sugar has been unable to meet the national sugar demand. The study of physiology, molecular biology and genetic is providing a major impetus to develop biotechnological strategies for increasing growth and productivity in sugarcane. Genetic transformation method for sugarcane has been established, including Agrobacterium-mediated transformation method. The Agrobacterium-mediated transformation has been successfully employed to develop transgenic sugarcane. The overexpression of SoSPS gene encoding for sucrosephosphate synthase (SPS) showed the increases of activity and sucrose content in transgenic sugarcane. Furthermore, field evaluation on growth and productivity of the transgenic sugarcane displayed higher tiller number, plant high, cane yield, percentage of Brix and Pol compared to nontransgenic sugarcane. Furthermore, plants are subjected to a variety of abiotic and biotic stresses, which reduces and limits crop productivity. Plants adapt to water stress with various strategies include change in the gene expression and accumulation of organic compounds called compatible solutes. Genetic transformation of betA gene encoding for choline dehydrogenase in bacteria elevated glycine-betaine content as an osmoprotectant and resulted in water stress tolerant of transgenic sugarcane. The drought tolerant of sugarcane was already approved and released by Indonesian Government for commercialization. In addition, mosaic virus is one of the most severe diseases in sugarcane and lead to the constant losses in growth and yield of sugarcane. Pathogen-derived resistance (PDR) and RNA interference (RNAi) technologies have been applied to engineered sugarcane cultivar resistant to mosaic virus, and that the RNAi method produced more resistant against the mosaic virus in sugarcane. Finaly, biotechnology approach of genome editing technology should be exploited to ensure higher sugarcane productivity, and improve the livelihoods of smallholder farmers.

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Increasing the Activity of Sugarcane Sucrose Phosphate Synthase Enhanced Growth and Grain Yields in Transgenic Indica Rice

et al. 2022

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Sucrose is the primary form of mobile photoassimilates, and its level is regulated by sucrose-phosphate synthase (SPS) in plants. Increasing in the SPS activity was accompanied by an increase in sucrose accumulation. This study was designed to examine the effect of the overexpression sugarcane SoSPS1 gene on sucrose metabolizing enzymes, growth, and grain yield of indica rice. The SoSPS1 gene was constructed in a binary vector under the control of a rice ubiquitin promoter and transformed into indica rice using an Agrobacterium vector. Five lines of transgenic rice were selected to develop homozygous transgenic lines and used for analysis. The overexpression of the SoSPS1 gene significantly increased the transcript and protein levels, followed by increasing in SPS activity and sucrose content in the leaves of the transgenic rice lines. Moreover, the activity of soluble acid invertase (SAI) was elevated rather than sucrose synthase (SuSy) in the transgenic lines. The increase in the sucrose-degrading enzymes leads to an increase in plant growth and development. The plant height and number of tillers were significantly higher in the transgenic line compared to non-transgenic (NT) rice. In addition, the amylose content, the number of seeds per panicle, and the weight of 1000 grains of seed, including dry biomass weight, were increased in the transgenic lines. The results indicated that enhancement of SPS activity, as well as sucrose content, provides a higher carbon partitioning for higher growth and productivity of the transgenic rice lines.

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Field Evaluation on Growth and Productivity of the Transgenic Sugarcane Lines Overexpressing Sucrose-Phosphate Synthase

Cited by 3 publications

References 30 publications

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Enhancing sugarcane growth quality and productivity through a biotechnology approach

Increasing the Activity of Sugarcane Sucrose Phosphate Synthase Enhanced Growth and Grain Yields in Transgenic Indica Rice

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