Field evidence of reproduction impairment through sperm DNA damage in the fish nase (Chondrostoma nasus) in anthropized hydrosystems

Devaux, Alain; Bony, Sylvie; Plénet, Sandrine; Sagnes, Pierre; Segura, Samuel; Suaire, Rémi; Novak, Morgane; Gilles, André; Olivier, Jean‐Michel

doi:10.1016/j.aquatox.2015.10.013

Cited by 30 publications

(17 citation statements)

References 53 publications

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“…Such impairments appear to be mediated by genetic or epigenetic changes to DNA in the sperm nucleus . For example, DNA damage in sperm positively correlated with offspring mortality or abnormality rates in other fishes . In addition, environmental condition and larval nutrition may also impact phenotypic plasticity and ontogeny of the skeleton .…”

Section: Discussionmentioning

confidence: 99%

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Outcomes of in vitro fertilization with frozen‐thawed sperm: An analysis of post‐thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Zadmajid

Falahipour

Ghaderi

et al. 2019

Developmental Dynamics

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Background Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are sired with fresh and frozen‐thawed sperm. Results Cryopreserved sperm did not affect embryogenesis at two‐ and four‐cell stages, but impaired embryonic development at eight‐cell stage. Embryonic viability decreased at organogenesis, where only 34‐49% of embryos showed viability with frozen‐thawed sperm. Hatching success and percentage of normal hatched embryos declined when fertilized with frozen‐thawed sperm. Considering only frozen‐thawed cells the dimethyl sulfoxide (DMSO)‐5%, methanol (METH)‐5%, and METH‐10% treatments yielded highest hatch, while METH‐5% and propylene glycol‐5% yielded the most normal hatched embryos. Larval spinal torsion was higher for fresh than frozen‐thawed sperm, where larvae with spinal torsion showed vertebral fusion and shape alterations during exogenous feeding. Both fresh and cryopreserved treatments showed abnormalities in caudal skeleton, while rates of defective yolk‐sacs were higher for cryopreserved sperm, where larvae with defective yolks showed oversized yolk extension. Percentage of larvae with defective heads/eyes were also higher for cryopreserved sperm. Conclusions Results show how frozen‐thawed sperm impairs embryonic/larvae development and identifies frequency and position of abnormalities. Future studies should investigate how sperm DNA damage may have caused these alterations.

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Section: Discussionmentioning

confidence: 99%

“…70,71 For example, DNA damage in sperm positively correlated with offspring mortality or abnormality rates in other fishes. 72,73 In addition, environmental condition and larval nutrition may also impact phenotypic plasticity and ontogeny of the skeleton. 74 Notochord abnormalities are frequently observed in newly hatched larval.…”

Section: Discussionmentioning

confidence: 99%

Outcomes of in vitro fertilization with frozen‐thawed sperm: An analysis of post‐thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Zadmajid

Falahipour

Ghaderi

et al. 2019

Developmental Dynamics

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“…It was shown that genotoxicity might be correlated with other relevant ecotoxicological responses such as as impairment of reproduction and growth in fish. Those defects are relevant in ecotoxicology since they provide information to relate individual effects to possible changes in population size or structure[32]. Even if one should notice in this work that the genotoxicity potential of effluents was rather low, whatever the treatment, adding genotoxicity assessment within a FELS test seems undoubtedly an added value, without the need for extra experimental facilities[33].…”

mentioning

confidence: 89%

Assessment of Ozone or Not-Treated Wastewater Ecotoxicity Using Mechanism-Based and Zebrafish Embryo Bioassays

Wigh¹,

Aı̈t-Aı̈ssa²,

Creusot³

et al. 2018

JEP

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Toxicity of wastewater treatment plant (WWTP) effluents is usually assessed with standardized bioassays, as e.g. the Fish Embryo Toxicity test (FET), but assessment of sub-lethal toxic effects requires to develop more adapted tests. The present work aimed to add the recording of several sub-lethal endpoints in exposed zebrafish embryo-larval stages in order to increase the sensitivity of residual toxicity evaluation of WWTP effluents using a semi-quantitative method (iFET score). This approach was complemented with the genotoxicity assessment on the exposed embryos, a sub-acute hazard particularly relevant to evaluate a potential chronic toxicity risk of low and multi-contaminated environmental matrices. Additionally, endocrine activities of effluents were quantified using human reporter cell lines. This test battery was applied to the assessment of the residual toxicity of five biological treatment effluents, further treated or not using various ozonation treatments intended to improve pharmaceutical compounds removal. Acute toxicity towards zebrafish embryos was very low. However, iFET score approach proposed was able to reveal residual toxicity through the presence of developmental abnormalities in all samples tested. Additionally, a low residual genotoxicity was measured in embryos exposed to two of the WWTP effluents when all excepted one exhibited a residual

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“…Our investigation focuses on spermatozoa since they are in most cases considered to be sensitive to contamination due to their inability to prevent oxidative stress and to repair DNA damage (Aitken et al, 2004). In addition, assessment of genotoxicity on this cellular type is of obvious interest regarding its key role in reproduction success (Lacaze et al, 2011a;Lewis and Galloway, 2010;Devaux et al 2011Devaux et al , 2015Santos et al, 2013a,b). During previous studies, the methodological procedure of Comet assay was adapted and optimized for Palaemon sp spermatozoa and a reference data distribution was defined for the coastal species P. serratus (Erraud et al, 2018 a,b).…”

Section: Mmentioning

confidence: 99%

Assessment of sperm DNA integrity within the Palaemon longirostris (H. ) population of the Seine estuary

Erraud¹,

Bonnard²,

Geffard³

et al. 2019

Environmental Pollution

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The interpretation of biomarkers in natura should be based on a referential of expected values in uncontaminated conditions. Nevertheless, to build a reference data set of biomarker responses in estuarine areas, which receive chronic pollution loads due to their transition position between continent and sea, is impossible. In this context, the aim of the present work was to propose the use of laboratory recovery period to define a baseline for the measurement of sperm DNA damage by Comet assay in the estuarine prawn Palaemon longirostris. For that, sperm DNA integrity was observed after both a passive (i.e. 20 days in a clean environment) and an active (i.e. forced renewal of spermatophores) recovery of wild P. longirostris specimens from the Seine estuary, in laboratory conditions. Then, the levels of sperm DNA damage recorded within the P. longirostris population of the Seine estuary, during six campaigns of sampling from April 2015 to October 2017, have been interpreted according to the defined threshold values. The results showed a persistence in the level of DNA damage after 20-day in clean environment with the passive recovery. This strategy was inconclusive to reach a baseline level but it revealed the lack of DNA repair mechanisms. For the active recovery, a decrease of 54 % of the level of DNA damage has been observed after the first renewal of spermatophores and this level stabilized after the second renewal. On the basis of this second strategy, we defined a mean basal value of sperm DNA damage of 54.9 A.U. and a maximum threshold of 69.7 A.U. (i.e. 95 %-CI). The analysis of the results using the reference value highlighted significant abnormal sperm DNA damage within the native population of P. longirostris from the Seine estuary on all stations during the six-sampling campaigns.

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Field evidence of reproduction impairment through sperm DNA damage in the fish nase (Chondrostoma nasus) in anthropized hydrosystems

Cited by 30 publications

References 53 publications

Outcomes of in vitro fertilization with frozen‐thawed sperm: An analysis of post‐thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Outcomes of in vitro fertilization with frozen‐thawed sperm: An analysis of post‐thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Assessment of Ozone or Not-Treated Wastewater Ecotoxicity Using Mechanism-Based and Zebrafish Embryo Bioassays

Assessment of sperm DNA integrity within the Palaemon longirostris (H. ) population of the Seine estuary

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