Field fertility of liquid stored and cryopreserved flow cytometrically sex‐sorted stallion sperm

Abstract

While the conception rates achieved are among the highest yet reported for sex-sorted, cryopreserved stallion sperm, the high incidence of pregnancy loss suggests that the development of the resulting embryos was significantly impaired by the sperm processing treatments.

Impact of supplementation of semen extender with antioxidants on quality of cooled or cryopreserved Marwari stallion spermatozoa

“…Cryopreservation of semen has created a new dimension in equine breeding industry by the possible preservation of this biological material for long time. However, compared with other domestic species, the fertility of frozen-thawed stallion sperm remained low (Gibb et al 2017). Cryopreservation of semen imposes some deleterious effects on spermatozoa, either killing a certain proportion of cells or causing damages on sperm function in the remaining surviving population (Yogesh et al 2018).…”

Clinical insights: Assisted reproductive technologies

“…However, while stallion spermatozoa can be sex‐sorted by flow‐cytometric detection of absolute DNA content after staining with a DNA dye (Hoechst 33342), and the chance of producing a foal when sorted sperm are used for AI freshly or after short‐term chilled storage is acceptable, the combination of sorting and cryopreserving the spermatozoa required to be able to distribute sex‐sorted semen more widely is still a work in progress. Although the latest study shows that AI with cryopreserved sex‐sorted sperm offers an acceptable chance of pregnancy, the viability of resulting pregnancies is compromised as indicated by a very high incidence of embryonic loss . This means that further refinement is required before sex‐sorting can be offered on a wider scale.…”