Field method to monitor the mycoparasitic fungus Coniothyrium minitans

Shukunami, Ryo; Iwamoto, Yutaka; Sugiura, Shinji; Ikeda, Kentaro; Nakayashiki, Hitohsi; Ikeda, Kenichi

doi:10.1007/s10327-015-0633-8

Cited by 2 publications

(2 citation statements)

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“…Sclerotinia sclerotiorum is a worldwide distributed destructive plant fungal pathogen, attacks more than 400 plant species (Boland and Hall, 1994) and causes huge economic losses (Schwartz and Singh, 2013). Coniothyrium minitans, a mycoparasitic fungus specific to some species in Sclerotinia (Campbell, 1947;Boland and Hall, 1994), parasitizes hypha and sclerotia of S. sclerotiorum efficiently and reduces the sclerotia inoculated in the soil (Campbell, 1947;Huang, 1977;Trutmann et al, 1980;Tu, 1984;Jiang et al, 1996;Li et al, 2006;Whipps et al, 2007;Shukunami et al, 2016). Therefore, C. minitans has aroused great interests for its biological control potential and has been developed as commercial biological control agents in many countries, including Germany, Russia, Spain, etc.…”

Section: Introductionmentioning

confidence: 99%

Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans

et al. 2020

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Mycoparasite Coniothyrium minitans parasitizes specifically the mycelia or sclerotia of Sclerotinia sclerotiorum, a worldwidely spread plant fungal pathogen causing serious diseases on crops. The interaction of C. minitans with S. sclerotiorum remains reciprocal and complex and little is known, especially on the side of the host (S. sclerotiorum). In this study, the early transcriptional response of S. sclerotiorum to the mycoparasitism by C. minitans was explored and the differentially expressed genes (DEGs) were analyzed. Based on GO ontology, KEGG pathway and fungal categories database, 887 upregulated DEGs were enriched in the growth related function (i.e., rRNA processing, ribosome biogenesis, binding and transport), while the 546 down-regulated DEGs were enriched in the stress-related functions (i.e., oxidoreductase, response to stress and heat and the chorismate biosynthetic process). The expression of shikimate pathway and the biosynthesis of phenylalanine involving genes was significantly suppressed. Furthermore, 581 unenriched DEGs were explored in the parasitizing process and were mapped on the Pfam domains of redox enzymes, Alpha/Beta hydrolase, haloacid dehalogenase, and other universal conserved domain containing proteins. Thirty-two DEGs encoding candidate effectors, with 16 up-regulated and 16 down-regulated, were observed with diverse function. SS1G_11912 (encoding SsNEP2) was significantly upregulated and may function in the parasitism. The involving of the shikimate pathway of phenylalanine biosynthesis and effector candidates were discussed. The results provide a basal understand on the interaction of S. sclerotiorum and C. minitans.

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Section: Introductionmentioning

confidence: 99%

Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans

et al. 2020

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“…In an alternative way, DNA sequence‐based methods can detect Armillaria directly from soil samples. PCR amplification of environmental DNA (eDNA) with taxa‐specific primers has been used to detect and monitor specific fungal species (Bridge & Spooner, ; Dauch, Watson, & Jabaji‐Hare, ; Shukunami et al., ; Zambounis, Paplomatas, & Tsaftaris, ). Because DNA sequence‐based methods can detect Armillaria and Desarmillaria in soils without extensive surveys, this approach provides more accurate information regarding the distribution and diversity of Armillaria and Desarmillaria species.…”

Section: Introductionmentioning

confidence: 99%

Re‐evaluation of Armillaria and Desarmillaria in South Korea based on ITS/tef1 sequences and morphological characteristics

Park

et al. 2018

Forest Pathology

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Fungal species in the genera Armillaria and Desarmillaria (Physalacriaceae, Agaricales) are well known for their symbiotic relationships with Gastrodia elata and Polyporus umbellatus, important components of traditional medicine in Asia. In addition, some species in these genera cause Armillaria root disease, which has had a negative economic impact by damaging and destroying urban, horticultural and forest trees. Five species within Armillaria and Desarmillaria have been previously reported in South Korea, based primarily on basidioma morphology: A. cepistipes, A. gallica, A. mellea, A. ostoyae and D. tabescens (reported as A. tabescens). This study re‐evaluated 60 specimens of Armillaria and Desarmillaria using morphological features and molecular phylogenetic analyses of the ITS and partial translation elongation factor‐1α (tef1) sequences. In addition, spatial distributions of each Armillaria and Desarmillaria species in South Korea were determined from combined data based on basidioma collections and environmental DNA (eDNA). Six species (A. cepestipes, A. gallica, A. mellea, A. nabsnona, A. ostoyae and D. tabescens) and three lineages of A. gallica (A. gallica clade 1–3) were identified in South Korea from both specimens and eDNA. Most of the basidioma specimens used in this study were previously identified as A. mellea; however, in this study, no basidioma specimens were identified as A. mellea, although this species was detected on Jeju Island from eDNA samples. The detection of A. nabsnona was a new report for South Korea. Consistent with previous phylogenetic studies, the tef1 analysis had better resolution than the ITS analysis. These distribution data of Armillaria and Desarmillaria species will contribute to better management of Armillaria root disease as well as cultivation of G. elata and P. umbellatus in South Korea.

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Field method to monitor the mycoparasitic fungus Coniothyrium minitans

Cited by 2 publications

References 18 publications

Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans

Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans

Re‐evaluation of Armillaria and Desarmillaria in South Korea based on ITS/tef1 sequences and morphological characteristics

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