Field performance evaluation and genetic integrity assessment in Argyranthemum ‘Yellow Empire’ plants recovered from cryopreserved shoot tips

Abstract

Field performance evaluation and genetic integrity assessment were conducted in Argyranthemum plants derived from cryopreserved shoot tips. Some variations in root formation and vegetative growth were found in the plants following cryopreservation, but morphologies of the leaves and flowers, and color, number and size of the flowers remained unchanged in the plants recovered from cryopreservation, compared with the control. Assessments of genetic integrity by the two molecular markers: inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) did not detect any polymorphic bands across the plants tested following cryopreservation. These data indicate that cryopreservation reduces, to a certain degree, root formation and vegetative growth, but it does not alter morphologies of leaves and flowers, may not cause any genetic alternations, and has no adverse effects on quantity and quality of the flowers. Therefore, the droplet vitrification cryopreservation can be considered promising for long-term preservation of Argyranthemum germplasm.

Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

“…However, there existed a few studies, in which field behavior of cryoderived plants differed from that of the control. Working on Argyranthemum, Zhang et al (2015) reported that root formation and plant growth were reduced in the cryo-derived plants grown in the greenhouse, produces more dependable results than those using only one marker (Wang et al 2014a(Wang et al , b, 2018. ISSR and AFLP did not detect any polymorphic bands in Solanum tuberosum plants cryopreserved by droplet-vitrification, encapsulation-vitrification and vitrification (Wang et al 2014b;Li et al 2017 (Ahuja et al 2002).…”

“…Final published version can be accessed at https://doi.org/10.1007/s11240-020-01820-7 Post-prints are subject to Springer Nature re-use terms ISSR was performed as described by Zhang et al (2015). Eight primers were selected from candidate primers, based on the number of amplified fragments in the range 500-2000 base pairs.…”

“…AFLP was conducted, according to Zhang et al (2015). Breifly, genomic DNA (300 ng) was double-digested with EcoRI and the MseI isoschizomer…”

“…Molecular markers like inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) have been widely used to assess genetic stability in cryo-derived regenerants of many plant species (Wang et al 2014a(Wang et al , b, 2017(Wang et al , 2018Li et al 2015;Zhang et al 2015;Bi et al 2016). However, such studies have been quite limited in cryo-derived regenerants of Allium spp.…”

Cryopreservation of Caninae rose shoot tips guarantees biochemical stability and pollination potential monitored in four-year-old regenerants

“…It is also important to evaluate field performance and plant functions, but up to now, studies on these topics are rather limited. There are works on papaya clones (Kaity et al 2009(Kaity et al , 2013 and Argyranthemum in the reproductive growth phase (Zhang et al 2015). Those observations did not prove any adverse effects on morphology and plant biochemical activity in regenerants.…”