Field performance of cell-suspension-derived Lolium perenne L. regenerants and their progenies

Stadelmann, F. J.; Boller, B.; Spangenberg, Germán; Kölliker, Roland; Wang, Z. Y.; Potrykus, Ingo; Nösberger, J.

doi:10.1007/s001220050782

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…Transgenic crops are now well established, occupying some 53 million ha outside Europe (James 2001). Methods of regenerating green plants from cell suspensions have been developed for perennial ryegrass, Italian ryegrass, tall fescue and cocksfoot (Dalton 1988;Horn et al 1988;Stadelmann et al 1998). Biolistics has been the most successful means of transforming grasses.…”

Section: Genetic Transformationmentioning

confidence: 99%

Progress in breeding perennial forage grasses for temperate agriculture

2003

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Wilkins, P. W., Humphreys, M. O. (2003). Progress in breeding perennial forage grasses for temperate agriculture. ? Journal of Agricultural Science, 140, (2), 129-150. Sponsorship: Germinal Holdings Ltd; Department for Agriculture, Food and Rural Affairs (DEFRA); Biotechnology and Biological Sciences Research Council (BBSRC)Breeding of temperate forage grasses is aimed at improving the economic and environmental sustainability of production from cattle and sheep. The primary objective is to ensure that forage can be the main source of feed for ruminants. This requires consistent production of herbage with a high feeding value, usually under nitrogen-limiting conditions. The most important traits affecting the feeding value of herbage are in vitro dry matter digestibility (DMD), the ratios of crude protein, water-soluble carbohydrate and fibre, and the concentration of alkaloid toxins. Improvements in these primary quality traits must be combined with good plant persistency, adequate tolerance to a range of environmental stresses, adequate resistance to a large number of different pathogens and invertebrate pests, and adequate seed yield. Forage grasses also have considerable potential to produce material for refining, to provide protein extracts for feeding to monogastric animals and carbohydrate for fermentation into fuel or into feedstocks for other industries.Peer reviewe

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Section: Genetic Transformationmentioning

confidence: 99%

Progress in breeding perennial forage grasses for temperate agriculture

2003

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“…However, to assess the suitability of biotechnological approaches in grass improvement programs, the agronomic performance of transgenic plants and their progenies must first be investigated. Moreover, such experiments must be carried out in the field to test the plants under conditions relevant to plant breeding (Stadelmann et al 1998b). To date, there are no reports on agronomic evaluations of transgenic forage grass plants under field conditions.…”

Section: Introductionmentioning

confidence: 99%

Field performance of transgenic tall fescue (Festuca arundinacea Schreb.) plants and their progenies

et al. 2003

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Tall fescue (Festuca arundinacea Schreb.) is a hexaploid, outcrossing grass species widely used for forage and turf purposes. Transgenic tall fescue plants were generated by biolistic transformation of embryogenic cell suspension cultures that were derived from single genotypes of widely used cultivar Kentucky-31. Primary transgenics from two genotypes, their corresponding regenerants from the same genotypes and control seed-derived plants were transferred to the field and evaluated for 2 years. Progenies of these three classes of plants were obtained and evaluated together with seed-derived plants in a second field experiment. The agronomic characteristics evaluated were: heading date, anthesis date, height, growth habit, number of reproductive tillers, seed yield and biomass. The agronomic performance of the primary transgenics and regenerants was generally inferior to that of the seed-derived plants, with primary transgenics having fewer tillers and a lower seed yield. However, no major differences between the progenies of transgenics and the progenies of seed-derived plants were found for the agronomic traits evaluated. Primary transgenics and regenerants from the same genotype were more uniform than plants from seeds. Progenies of transgenics performed similarly to progenies of the regenerants. The addition of a selectable marker gene in the plant genome seems to have had little effect on the agronomic performance of the regenerated plants. No indication of weediness of the transgenic tall fescue plants was observed. Our results indicate that outcrossing grass plants generated through transgenic approaches can be incorporated into forage breeding programs.

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“…Compared with non-transformed control plants transgenecontaining plants showed, on average, a delay in heading (37.3 days vs. 29.9 days after the first LD) (Table 1, Expt A). It is known that in Lolium and other monocot species, tissue culture and particle bombardment can cause late heading because of chromosomal changes (Stadelmann et al 1998, Choi et al 2000a. Indeed, late heading was also observed in some of the transformants lacking the transgene (Table 1, Expt A) or lacking transgene expression (data not shown).…”

Section: Resultsmentioning

confidence: 90%

Late heading of perennial ryegrass caused by introducing an Arabidopsis homeobox gene

et al. 2004

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Perennial ryegrass (Lolium perenne L.) is the most important temperate forage grass species. Unfortunately, the nutritional value of perennial ryegrass declines as maturity progresses, mainly because of a high concentration of poorly digestible compounds in inflorescences. Therefore, the development of forage-type ryegrass varieties with extended vegetative growth is of interest for agriculture. To delay floral transition in perennial ryegrass the Arabidopsis ATH1 gene driven by the maize ubiquitin promoter, the rice actin promoter or the rice OSH1 promoter, respectively was introduced. In ATH1-expressing plants heading was delayed, and in a number of cases the plants never flowered at all. Such non-or late-heading was accompanied by the outgrowth of normally quiescent lateral meristems into extra leaves, resulting in a leafy growth habit. When eventually heading, these plants generally produced a reduced number of inflorescences. These observations suggest that ATH1-mediated delay of heading may be useful to improve fodder quality of perennial ryegrass.

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Field performance of cell-suspension-derived Lolium perenne L. regenerants and their progenies

Cited by 8 publications

References 8 publications

Progress in breeding perennial forage grasses for temperate agriculture

Progress in breeding perennial forage grasses for temperate agriculture

Field performance of transgenic tall fescue (Festuca arundinacea Schreb.) plants and their progenies

Late heading of perennial ryegrass caused by introducing an Arabidopsis homeobox gene

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